LOCUS       sequence-314360-       10863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of myc-tagged Opa1 isoform 7 Delta S1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10863)
  AUTHORS   Mishra P, Carelli V, Manfredi G, Chan DC
  TITLE     Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane
            fusion and couples fusion to oxidative phosphorylation.
  JOURNAL   Cell Metab. 2014 Apr 1;19(4):630-41. doi: 
            10.1016/j.cmet.2014.03.011.
  PUBMED    24703695
REFERENCE   2  (bases 1 to 10863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2014.03"; journalName: "Cell Metab"; date: 
            "2014-04-1- 1"; volume: "19"; issue: "4"; pages: "630-41"
COMMENT     Sequence Label: pclbw-opa1(isoform 7DeltaS1)-myc
FEATURES             Location/Qualifiers
     source          1..10863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          110..1126
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1050..1072
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1134..1153
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1214..1238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    1394..1418
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4395..4424
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4431..4460
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4467..4496
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4515..4544
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4551..4580
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4587..4616
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4635..4664
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4671..4700
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4707..4736
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(4779..4803)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4848..5436
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4901..4921)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5319..5330)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5439..5455)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5440..5456)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5773..6242
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6372..6389)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6543..7131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6623..6642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7291..8082,8083..8151))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7914..7933
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        8303..8682
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8683..8886
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8837..8857
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    9125..9482
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             9547..9963
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     9893..9915
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     9931..9947
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    9973..10347
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     10285..10301
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     enhancer        10448..10751
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggggggk gcggcggcag ccarkcagrg cggcgcgctc cgaaagtttc cttttatggc
       61 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
      121 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      181 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
      241 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      301 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
      361 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
      421 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
      481 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
      541 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
      601 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
      661 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
      721 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
      781 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
      841 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
      901 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
      961 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     1021 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     1081 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     1141 gctggttatt gtgctgtctc atcattttgg caaagaattg atttgatacc gcgggcccgg
     1201 gatccctcga atcacaagtt tgtacaaaaa agcaggctac catgtggcga gcaggtcggg
     1261 cggccgtggc ctgtgaagtc tgccaatcct tagtgaaaca cagctctgga ayacmaasrw
     1321 rckkrttatt gtgctgtctc atcattttgg caaagaattg atttgatacc gcgggcccgg
     1381 gatccctcga atcacaagtt tgtacaaaaa agcaggctac catgtggcga gcaggtcggg
     1441 cggccgtggc ctgtgaagtc tgccaatcct tagtgaaaca cagctctgga atacaaagaa
     1501 acgtaccgct ccaaaaactc catctggttt cacgaagtat ttatcgttca catcatcctg
     1561 ccctcaagct tcaaagaccc caactaagga caccatttca gcagttctct tctctaactc
     1621 acctttcatt acataaattg aaactttctc caattaaata tggctaccag ccccgcagga
     1681 acttttggcc agcaaggtta gctgcaagac tcttaaaact tcgatatatc atactgggat
     1741 ctgctgttgg aggtggctat acagccaaaa aaaccttcga tgaatggaaa gatatgatac
     1801 cagaccttag tgactataag tggattgtgc ctgactttat atgggaaatt gatgagtata
     1861 ttgatttgga gaaaattaga aaagcccttc ccagctcaga agaccttgcc agtttagctc
     1921 ccgacttgga caagattact gagagcctca gcttgttgaa ggacttcttc actgcaggtt
     1981 cacctggaga aagtgacaag cattacagga agggtctgct tggtgagctc attctcttac
     2041 aacagcaaat tcaagagcac gaagaggaag cacgcagagc cgctggccag tatagcacga
     2101 gctatgccca acagaagcgc aaggtgtcag acaaagaaaa gattgaccaa cttcaagaag
     2161 aacttctgca tactcagtta aagtatcaga ggatcttgga gcgcctggaa aaggagaaca
     2221 aagagctgag gaagctggtg ctgcagaagg acgacaaagg catccaccac aggaagctca
     2281 agaaatcttt gattgatatg tattctgaag ttcttgatgt tctttctgat tatgatgcca
     2341 gttacaatac acaagatcac ctaccacggg ttgttgtggt tggagatcag agtgctggga
     2401 aaaccagtgt gctggaaatg attgctcagg cccggatctt cccgagaggg tccggcgaga
     2461 tgatgacacg ctctccagtg aaggtgactc tcagtgaagg ccctcaccat gtggccttgt
     2521 ttaaagatag ctctcgggaa tttgatctca ccaaggagga agatcttgca gcattaagac
     2581 atgaaatcga actccgaatg aggaaaaatg tgaaagaagg ttgtactgtt agccccgaga
     2641 ccatatctct aaatgtcaaa ggccctgggc tgcagaggat ggtgctcgtg gacttgcctg
     2701 gtgtcatcaa cactgtgaca tcaggcatgg ctcccgacac aaaggaaact attttcagta
     2761 tcagcaaagc ttacatgcag aatcctaacg ccatcatcct gtgcatccaa gacggatccg
     2821 tagatgctga gcgcagtatt gttacagact tggtcagtca aatggatcct catggaagaa
     2881 gaaccatatt tgttttgacc aaagtagacc tggcagaaaa aaatgtagcc agtccaagca
     2941 ggatacaaca gataattgaa ggcaagctct tcccaatgaa agctctgggt tattttgctg
     3001 tcgtaacagg aaaaggaaac agctctgaaa gtattgaagc tataagagag tatgaagagg
     3061 aattttttca gaattcaaaa ctgctaaaga caagcatgct aaaggcacac caggtcacca
     3121 cgagaaatct cagccttgct gtgtcagact gcttttggaa aatggttcga gagtcagttg
     3181 aacaacaggc tgatagtttt aaagccacgc gctttaacct agagacggaa tggaagaata
     3241 actacccgcg cctgcgagag ctcgacagga atgaactctt tgaaaaagct aaaaatgaga
     3301 tcctcgatga ggtcatcagt ctgagccagg tcacgccaaa gcactgggag gaaatcctgc
     3361 agcaatccct gtgggaacga gtgtcaacac atgtgattga gaacatctac cttccagctg
     3421 cccagaccat gaattcggga acatttaaca ccacagtaga catcaagctt aaacagtgga
     3481 ctgacaagca gcttcctaat aaagcagtcg aggttgcctg ggagactcta caagaggaat
     3541 tttcccgctt catgacagaa cccaaaggaa aggaacacga cgacatattt gacaaactta
     3601 aggaggctgt gaaggaggag agtatcaagc ggcacaagtg gaacgacttt gccgaggata
     3661 gcttgagggt tattcagcac aatgctttgg aagaccggtc catatcagat aagcaacagt
     3721 gggatgcagc catttacttc atggaagagg cgcttcaagg tcgtctcaag gatactgaaa
     3781 atgctattga aaacatgatt gggccagact ggaaaaagag gtggatgtac tggaagaatc
     3841 ggacccaaga gcagtgtgtt cacaacgaaa ccaagaacga gttggagaag atgctgaagg
     3901 ttaatgatga gcacccagct tacctggcaa gtgatgagat taccacagtc cggaagaacc
     3961 tggagtctcg aggagtggaa gtcgatccaa gcttgattaa ggatacttgg catcaagttt
     4021 atagaagaca tttcttaaaa acagctctaa atcattgtaa cctttgtcgc agaggttttt
     4081 attactacca gaggcatttt atagattctg agctggaatg caatgacgtg gtcctgtttt
     4141 ggcgaataca gcgcatgctc gctatcactg ccaatacatt aaggcagcag cttacaaaca
     4201 ctgaagttag gcgactagag aaaaacgtta aagaggtatt agaagatttt gcagaagacg
     4261 gtgagaagaa ggttaaattg ctcactggca aacgagttca gctggcagaa gatctcaaga
     4321 aagttagaga aattcaagaa aagcttgatg ctttcattga agctcttcac caggagaagg
     4381 gaggaacgag tggtgaacaa aagttgattt ctgaagaaga tttgaacggt gaacaaaagc
     4441 taatctccga ggaagacttg aacggtgaac aaaaattaat ctcagaagaa gacttgaacg
     4501 gatcctctag aggtgaacaa aagttgattt ctgaagaaga tttgaacggt gaacaaaagc
     4561 taatctccga ggaagacttg aacggtgaac aaaaattaat ctcagaagaa gacttgaacg
     4621 gatcctctag aggtgaacaa aagttgattt ctgaagaaga tttgaacggt gaacaaaagc
     4681 taatctccga ggaagacttg aacggtgaac aaaaattaat ctcagaagaa gacttgaacg
     4741 gatccactag tgggggcgaa aacttgtatt ttcaataaac ccagctttct tgtacaaagt
     4801 ggtgattcga ggccgcgact ctagaattcg atatcaagct tatcgataat caacctctgg
     4861 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4921 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4981 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5041 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5101 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5161 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5221 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5281 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5341 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5401 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgatcga
     5461 ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata
     5521 ataaccgggc aggccatgtc tgcccgtatt tcgcgtaagg aaatccatta tgtactattt
     5581 tcgagcggcc gccagcacag tggtcgacga taaaataaaa gattttattt agtctccaga
     5641 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     5701 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     5761 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     5821 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     5881 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
     5941 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     6001 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     6061 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     6121 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     6181 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     6241 cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg
     6301 ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6361 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6421 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6481 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6541 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6601 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6661 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6721 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6781 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6841 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6901 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6961 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7021 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7081 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7141 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7201 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccgca aatcaatcta
     7261 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7321 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7381 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7441 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7501 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7561 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7621 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7681 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7741 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7801 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7861 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7921 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7981 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8041 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8101 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8161 ttttcaatga tccggccatt agccatatta ttcattggtt atatagcata aatcaatatt
     8221 ggctattggc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
     8281 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
     8341 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     8401 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     8461 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     8521 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     8581 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     8641 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     8701 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     8761 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     8821 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     8881 agagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga
     8941 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     9001 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     9061 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
     9121 aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt
     9181 ttatgcgcct gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg
     9241 gaactgacga gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg
     9301 gccgtttttg tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca
     9361 ccccccttag aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg
     9421 cctccgtctg aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc
     9481 tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg
     9541 ggcccgggcc agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc
     9601 gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc
     9661 tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac
     9721 ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag
     9781 gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc
     9841 tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa
     9901 cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc
     9961 gcccccatat ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct
    10021 gaccctgaca tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc
    10081 tacttagtcc agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac
    10141 cgaccggtgg tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag
    10201 actaagaacc tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc
    10261 gccctcaaag tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac
    10321 cccgggggtg gaccatcctc tagactgccg gatcccagtg tggtggtacg ggaattctgc
    10381 aggaatctag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    10441 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
    10501 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
    10561 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
    10621 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
    10681 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
    10741 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
    10801 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
    10861 ggg
//
