LOCUS       sequence-314364-        8853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of Tn5-APEX2 fusion protein, no linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Lee JD, Paulo JA, Posey RR, Mugoni V, Kong NR, Cheloni G, Lee YR, 
            Slack FJ, Tenen DG, Clohessy JG, Gygi SP, Pandolfi PP
  TITLE     Dual DNA and protein tagging of open chromatin unveils dynamics of 
            epigenomic landscapes in leukemia.
  JOURNAL   Nat Methods. 2021 Mar;18(3):293-302. doi: 
            10.1038/s41592-021-01077-8. Epub 2021 Mar 1.
  PUBMED    33649590
REFERENCE   2  (bases 1 to 8853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-021-01077-8"; journalName: "Nat Methods"; date: 
            "2021-03"; volume: "18"; issue: "3"; pages: "293-302"
COMMENT     Sequence Label: pTXB1-TP1
FEATURES             Location/Qualifiers
     source          1..8853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(142..333)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(890..1972)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1934..1953
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1973..2050)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      2203..2289
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2386..2472
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2569..2655
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2752..2838
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2935..3021
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3156..3175
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3156..3174
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3175..3199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3243..4670
                     /codon_start=1
                     /product="transposase from the bacterial Tn5 transposon 
                     (Reznikoff, 1993)"
                     /label=Tn5 transposase
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     CDS             4671..5417
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             5418..5441
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5442..6035
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             6066..6221
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     primer_bind     complement(6286..6304)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6300..6347
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6407..6511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6512..6580,6581..7372)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6730..6749)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7414..7927)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7609..7630)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7821..7840
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      8038..8626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8527..8546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8780..8797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8819..8838)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
       61 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      121 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
      181 gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc
      241 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
      301 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
      361 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
      421 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
      481 gcgggaccag agaaaaatca ctcagggtca atgccagccg aacgccagca agacgtagcc
      541 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
      601 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
      661 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
      721 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
      781 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
      841 agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc
      901 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      961 aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca
     1021 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt
     1081 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
     1141 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg
     1201 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
     1261 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
     1321 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
     1381 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
     1441 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
     1501 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag
     1561 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
     1621 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
     1681 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
     1741 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
     1801 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
     1861 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
     1921 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca
     1981 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
     2041 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc
     2101 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg ccggcatgcc
     2161 gccctttcgt cttcaagaat taattcccaa ttccccaggc atcaaataaa acgaaaggct
     2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     2281 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     2341 gcaggacgcc cgccataaac tgccaggaat taattcccca ggcatcaaat aaaacgaaag
     2401 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     2461 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     2521 cgggcaggac gcccgccata aactgccagg aattaattcc ccaggcatca aataaaacga
     2581 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2641 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     2701 tggcgggcag gacgcccgcc ataaactgcc aggaattaat tccccaggca tcaaataaaa
     2761 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2821 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
     2881 gggtggcggg caggacgccc gccataaact gccaggaatt aattccccag gcatcaaata
     2941 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     3001 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     3061 ggagggtggc gggcaggacg cccgccataa actgccagga attggggatc ggaattaatt
     3121 cccggtttaa accggggatc tcgatcccgc gaaattaata cgactcacta taggggaatt
     3181 gtgagcggat aacaattccc ctctagaaat aattttgtta actttaagaa ggagatatac
     3241 atatgattac cagtgcactg catcgtgcgg cggattgggc gaaaagcgtg ttttctagtg
     3301 ctgcgctggg tgatccgcgt cgtaccgcgc gtctggtgaa tgttgcggcg caactggcca
     3361 aatatagcgg caaaagcatt accattagca gcgaaggcag caaagccatg caggaaggcg
     3421 cgtatcgttt tattcgtaat ccgaacgtga gcgcggaagc gattcgtaaa gcgggtgcca
     3481 tgcagaccgt gaaactggcc caggaatttc cggaactgct ggcaattgaa gataccacct
     3541 ctctgagcta tcgtcatcag gtggcggaag aactgggcaa actgggtagc attcaggata
     3601 aaagccgtgg ttggtgggtg catagcgtgc tgctgctgga agcgaccacc tttcgtaccg
     3661 tgggcctgct gcatcaagaa tggtggatgc gtccggatga tccggcggat gcggatgaaa
     3721 aagaaagcgg caaatggctg gccgctgctg caacttcgcg tctgagaatg ggcagcatga
     3781 tgagcaacgt gattgcggtg tgcgatcgtg aagcggatat tcatgcgtat ctgcaagata
     3841 aactggccca taacgaacgt tttgtggtgc gtagcaaaca tccgcgtaaa gatgtggaaa
     3901 gcggcctgta tctgtatgat cacctgaaaa accagccgga actgggcggc tatcagatta
     3961 gcattccgca gaaaggcgtg gtggataaac gtggcaaacg taaaaaccgt ccggcgcgta
     4021 aagcgagcct gagcctgcgt agcggccgta ttaccctgaa acagggcaac attaccctga
     4081 acgcggtgct ggccgaagaa attaatccgc cgaaaggcga aaccccgctg aaatggctgc
     4141 tgctgaccag cgagccggtg gaaagtctgg cccaagcgct gcgtgtgatt gatatttata
     4201 cccatcgttg gcgcattgaa gaatttcaca aagcgtggaa aacgggtgcg ggtgcggaac
     4261 gtcagcgtat ggaagaaccg gataacctgg aacgtatggt gagcattctg agctttgtgg
     4321 cggtgcgtct gctgcaactg cgtgaatctt ttactccgcc gcaagcactg cgtgcgcagg
     4381 gcctgctgaa agaagcggaa cacgttgaaa gccagagcgc ggaaaccgtg ctgaccccgg
     4441 atgaatgcca actgctgggc tatctggata aaggcaaacg caaacgcaaa gaaaaagcgg
     4501 gcagcctgca atgggcgtat atggcgattg cgcgtctggg cggctttatg gatagcaaac
     4561 gtaccggcat tgcgagctgg ggtgcgctgt gggaaggttg ggaagcgctg caaagcaaac
     4621 tggatggctt tctggccgcg aaagacctga tggcgcaggg cattaaaatc ggaaagtctt
     4681 acccaactgt gagtgctgat taccaggacg ccgttgagaa ggcgaagaag aagctcagag
     4741 gcttcatcgc tgagaagaga tgcgctcctc taatgctccg tttggcattc cactctgctg
     4801 gaacctttga caagggcacg aagaccggtg gacccttcgg aaccatcaag caccctgccg
     4861 aactggctca cagcgctaac aacggtcttg acatcgctgt taggcttttg gagccactca
     4921 aggcggagtt ccctattttg agctacgccg atttctacca gttggctggc gttgttgccg
     4981 ttgaggtcac gggtggacct aaggttccat tccaccctgg aagagaggac aagcctgagc
     5041 caccaccaga gggtcgcttg cccgatccca ctaagggttc tgaccatttg agagatgtgt
     5101 ttggcaaagc tatggggctt actgaccaag atatcgttgc tctatctggg ggtcacacta
     5161 ttggagctgc acacaaggag cgttctggat ttgagggtcc ctggacctct aatcctctta
     5221 ttttcgacaa ctcatacttc acggagttgt tgagtggtga gaaggaaggt ctccttcagc
     5281 taccttctga caaggctctt ttgtctgacc ctgtattccg ccctctcgtt gacaaatatg
     5341 cagcggacga agatgccttc tttgctgatt acgctgaggc tcaccaaaag ctttccgagc
     5401 ttgggtttgc tgatgccgat tacaaggatg acgacgataa gtgcatcacg ggagatgcac
     5461 tagttgccct acccgagggc gagtcggtac gcatcgccga catcgtgccg ggtgcgcggc
     5521 ccaacagtga caacgccatc gacctgaaag tccttgaccg gcatggcaat cccgtgctcg
     5581 ccgaccggct gttccactcc ggcgagcatc cggtgtacac ggtgcgtacg gtcgaaggtc
     5641 tgcgtgtgac gggcaccgcg aaccacccgt tgttgtgttt ggtcgacgtc gccggggtgc
     5701 cgaccctgct gtggaagctg atcgacgaaa tcaagccggg cgattacgcg gtgattcaac
     5761 gcagcgcatt cagcgtcgac tgtgcaggtt ttgcccgcgg gaaacccgaa tttgcgccca
     5821 caacctacac agtcggcgtc cctggactgg tgcgtttctt ggaagcacac caccgagacc
     5881 cggacgccca agctatcgcc gacgagctga ccgacgggcg gttctactac gcgaaagtcg
     5941 ccagtgtcac cgacgccggc gtgcagccgg tgtatagcct tcgtgtcgac acggcagacc
     6001 acgcgtttat cacgaacggg ttcgtcagcc acgctactgg cctcaccggt ctgaactcag
     6061 gcctcacgac aaatcctggt gtatccgctt ggcaggtcaa cacagcttat actgcgggac
     6121 aattggtcac atataacggc aagacgtata aatgtttgca gccccacacc tccttggcag
     6181 gatgggaacc atccaacgtt cctgccttgt ggcagcttca atgactgcag gaaggggatc
     6241 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
     6301 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
     6361 ctatatccgg ataactacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6421 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6481 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     6541 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     6601 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     6661 cagcggtaag atccttgaga gttttcgccc cgaagaacgt ttcccaatga tgagcacttt
     6721 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     6781 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     6841 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     6901 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6961 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7021 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     7081 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7141 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     7201 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     7261 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     7321 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     7381 ccaagtttac tcatatatac tttagattga tttaccccgg ttgataatca gaaaagcccc
     7441 aaaaacagga agattgtata agcaaatatt taaattgtaa acgttaatat tttgttaaaa
     7501 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     7561 atcccttata aatcaaaaga atagcccgag atagggttga gtgttgttcc agtttggaac
     7621 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     7681 ggcgatggcc cactacgtga accatcaccc aaatcaagtt ttttggggtc gaggtgccgt
     7741 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     7801 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     7861 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctatag
     7921 ggcgcgtaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7981 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8041 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8101 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8161 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     8221 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8281 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8341 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8401 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8461 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8521 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8581 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8641 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8701 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8761 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gctatggtgc actctcagta
     8821 caatctgctc tgatgccgca tagttaagcc agt
//
