LOCUS       sequence-314370-       10085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of Ost4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Jiang Z, Jin X, Li Y, Liu S, Liu XM, Wang YY, Zhao P, Cai X, Liu Y, 
            Tang Y, Sun X, Liu Y, Hu Y, Li M, Cai G, Qi X, Chen S, Du LL, He W
  TITLE     Genetically encoded tags for direct synthesis of EM-visible gold 
            nanoparticles in cells.
  JOURNAL   Nat Methods. 2020 Sep;17(9):937-946. doi: 10.1038/s41592-020-0911-z.
            Epub 2020 Aug 10.
  PUBMED    32778831
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0911-z"; journalName: "Nat Methods"; date: 
            "2020-09"; volume: "17"; issue: "9"; pages: "937-946"
COMMENT     Sequence Label: pDUAL-Pnmt1-Ost4-GFP
FEATURES             Location/Qualifiers
     source          1..10085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1733..2446
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2635..2822
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2690..2714)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4486..5280)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      5889..6672
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(7395..7412)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7395..7411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7404..7426)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7624..8079
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7711..7730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7921..7942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8096..8115)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8215..8237
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8275..8293)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8361..8465
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8466..8534,8535..9326)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8684..8703)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9497..10085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9986..10005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
      421 gcatgcctgc aggtcgatcg actctagagg atcagaaaat tatcgccata aaagacagaa
      481 taagtcatca gcggttgttt catttcctat attttttttt tattttttta ttttttaata
      541 agggaaaatt taacgtctaa ggatacagaa gattgttagc acattaaagt aataaaggct
      601 taagtagtaa gtgccttagc atgttattgt atttcaaagg acataatcta aaataataac
      661 aatatcattt ctcacaagtt attcaatttt cttttttttt tctaataata tcaagaatgt
      721 attatttgtt tgacataagt caactaattt atttaatatg ctggattaat cttgcagaca
      781 tgtaaattaa caagttttag tcaaataacg ttgaagtttc aatgaactca aataatttct
      841 cttttttttt atataaccat aatctgattt atattttccg cagggatcaa ctgaagttat
      901 gacatttgga ttggatcact tataaccttg gtcgccaaat aatacaaaaa tcagcgttat
      961 aaaacaaaga aggtttttgt taagaaatta atcctctttc ttgataagaa agttgaaccg
     1021 aaattgcaga tactgatata tgaaaataat acccacaatt ttgggaatag cgcaagcctc
     1081 aatttaaaca ataggtgagg acacatgata atgacctcaa tgattgttag aagaaaagag
     1141 cctcattaca aaatcgaaaa atgaatggtt gggtacaagt ttccaaaaca tggtaaagtg
     1201 gactttgcgt atgagacgta aatagaaaaa aacacttgtt atatgttttc tagaattatt
     1261 gttgtctctt tatggttgga tgatgcaaaa tagtaatttc ggttagttgc tgtaaaacac
     1321 cacgagacaa atagatatgg atatttatta aatcaggaaa aacgtaactc tcggctactg
     1381 gatggttcag tcacccaacg attactgggg agagaaaaca gggcaaaagc aaagcttaaa
     1441 ggaatccgat tgtcattcgg caatgtgcag cgaaactaaa aaccggataa tggacctgtt
     1501 aatcgaaaca ttgaagatat ataaaggaag aggaatcctg gcatatcatc aattgaataa
     1561 gttgaattaa ttatttcaat ctcattctca ctttctgact tatagtcgct ttgttaaagc
     1621 tagcggatcc atgacagacg ttcaacttca aaatatcgtt accacctttg gtatctccat
     1681 gatgctctta atcatccttt accattacct ttcacgtcct caagcaggat ccatgtctaa
     1741 aggtgaagaa ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa
     1801 tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac
     1861 cttaaaattt atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac
     1921 tttcggttat ggtgttcaat gttttgcgag atacccagat catatgaaac aacatgactt
     1981 tttcaagtct gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga
     2041 cggtaactac aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat
     2101 cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata
     2161 caactataac tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagt
     2221 taacttcaaa attagacaca acattgaaga tggttctgtt caattagctg accattatca
     2281 acaaaatact ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccac
     2341 tcaatctgcc ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt
     2401 tgttactgct gctggtatta cccatggtat ggatgaattg tacaaaagat ctatgagtcc
     2461 tccgaagccg acctgcgcct gcggtatctc cggctctagc ccaaccctca ccacctgccg
     2521 gaactccgcc tgtccttgct acaagagtta caacagctgt gccgggtgtc attgcgtggg
     2581 ctgcaccaat ccgcacaagg agacctaaag atctggcgcg ccacttctaa ataagcgaat
     2641 ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa
     2701 ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg
     2761 taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc
     2821 ggcagatccg ctagggataa cagggtaata tactagtaag atgattcttt tatcgtcgat
     2881 aacgatgagg atgtagacga ttatgatgag agtgattagc ttctaaaatt tggtgttttc
     2941 aaacgctatt tacagtacaa tctgaaaaaa ttggatgaac attgtaaatg gtaggatacg
     3001 agttccaatt aatgtatttt ttaagtatta cacttagctt attaatttta tatatacatt
     3061 ctacctctgt gggtttaaag acaactcgta cacatactca aattatttat gaacattata
     3121 atgcatattc gtactgtcta ttaaacattt ttttgacatt aaaaatttcg tttactaacg
     3181 tagaaagcgc tgataattga ggctactatt ttaatatcta gaaacaaaga ttcattaatt
     3241 aattaagtaa gctccaggat acttgtatat ttcgttaaaa aagcgcaatt tcaacaattc
     3301 ctatgaacat ccatgatttc atcacaaaat caaacatatc aggatttaac gtgaattatt
     3361 tgctttcttg tgattttcat ctgatttctg gtcatttacg ttactgtaaa caagcttttt
     3421 aaaaaaagga ttttttacgc aatattatcg atatcccaat ctgtagtata aaaagtgcaa
     3481 cagtgttgag tttcccgaaa ccaggcattt ttgcggtagg gtctgaaact ttcacgaaac
     3541 catttcacaa caaaacttct tcaacaacat gtgtgcaaag aaaatcgtcg tcttaccagg
     3601 agaccatatt ggccctgaaa ttgttgcttc tgccttggag gttttgaaag tcgttgagaa
     3661 gaagcgacct gagttaaaac tcgagtttga agaacacaag attggaggtg cctctattga
     3721 tgcctatgga acccctttga ctgacgagac tgtgaaggct tgtttggaag ctgacggtgt
     3781 tcttttgggt gccgttggtg gtcctgaatg gaccaacccc aattgtcgtc ctgagcaagg
     3841 tttattgaag cttcgtaaga gtatgggtgt ttgggccaac cttcgacctt gcaactttgc
     3901 cagcaagtct ttagtcaagt acagcccttt gaagcctgaa atcgttgaag gtgtcgattt
     3961 ttgtgttgta cgagaactta ctggaggttg ttactttggt gagcgcactg aggacaacgg
     4021 atcgggttat gctatggaca cttggcctta cagtttggaa gaagtttctc gtattgctcg
     4081 tttggcggcc gcgggcccaa caccaatgtt tataaccaag ttttatcttg tttgtctaca
     4141 tggtatttta cattcatcta catacatctt tcattggctt tgtacatagt tatcattaca
     4201 agtctaaaaa aattcactct tttcttattc aatgtcaatc caagagaaaa gattgtggta
     4261 atgttgtagg agcatgttta ataaattact atagcaaatt actttttatt cccaaggtgt
     4321 ttatctataa tagttaatat tttagtcgct acataaaatt ttaccaaaga gtacttgtat
     4381 actaattcta aatgccttct gacataaaac gcctaggaaa acaaacgcaa acaaggcatc
     4441 gactttttca ataaccaacc aaaaaaattt tacattagtc tttttttaat gctgagaaag
     4501 tctttgctga tatgccttcc aaccagcttc tctatatctc ttggcttcga caacaggatt
     4561 acgaccagct ccatagactc cacgaccaac aatgatgatg tcgctaccgc agtttacaat
     4621 cacttcttca ggagtacgat attgctgtcc cagcccgtct cctttaacat ccaagccgat
     4681 accaggggac atagttatgt agtcgctttg aaggttagga aatcgacgac cagctataaa
     4741 gccaaagcaa aaatcggtat gcttctcaaa ccattctaag gttttctctg tgtaggaacc
     4801 agtagccaaa gagcctttgg aagacatttc agccaaaagc aagagaccac gtcccaaagg
     4861 taaaccaact tctttgaggc cttgtataat accctcgcct ggcactgtat ggcaatttgt
     4921 gatatgagcc caagaagcaa ttttgtacac accagatgca tattgtagct tgacggtatt
     4981 tccaatgtct gcgaatttgc gatcctcaaa gataagaaaa cgatgctttt tacctaaggc
     5041 caccagtttt tctaccatat cctggtcgaa atcctcgaca acgtcaatat gtgtcttgat
     5101 aacacagaca tagggtccaa ttttatctac caattctaag atttcggatt tcttcgtcaa
     5161 atcgaccgcg actgacaagt tgctttgctt ttcttccatc aaagccaaca attccttggc
     5221 aatgggattt ttcatcccct cagctctagc tgaatagctt tgaaatactc tagcatccat
     5281 aactttgctt ttaaaccttt aatttcgatc caagcaaaaa agaggttctt ggtaggacaa
     5341 tacggtaaga aaacacgaca tgtgcagaga tgccgacgaa gcatagttaa actgggatgg
     5401 taaaatcaat taagaattta taaagacaaa attgtataag tctctaaaac atcttaatta
     5461 tacctcacag aactatctaa aatatattca caaagtgcaa acattatcat gaaaaagaac
     5521 cattttaatt taaagcaagg gcattaaggc ttatttacag aatttcttac ttttgtaaag
     5581 attataaggc tgattatctt tttcaccatg ccaaaaatta cacaagatag aatggatgtt
     5641 tgaaattaaa cgtgagtata caaacaaata cactaggtaa atcgaaacat ttttttctcc
     5701 attaagtaac aaattcctat ttagagaaag aatgctgagt agattaaata atctatacaa
     5761 acttttttaa cacaaatgca tacatatagc cagtgggatt tgtagctaag cttgcgaatt
     5821 cgagtctaac tccttaacca ctcggacata gtgacttatc tgacactcat tgtaaattaa
     5881 tatatatata ggcattttgt ttagttaaag gtacttaagt aattagtata aacgaaccaa
     5941 ttttataatc aggaagttaa gtgaatggta gcacatgtcg taaaaattgt gaatttttat
     6001 tgaataatat tttaaataca agcctttcta gactaggtat actcataaac atatatgagc
     6061 aaaaggatag aggagattac attgcatctt ctacaaattt atttattgcc ctttactgaa
     6121 aaattaaata atgagtacta aatgataaaa agcgctcagt acaagaaaga ttgcaaaaat
     6181 attgcattct tcatgaatta aagttgcata taaggcatat tgaaagtaat agtactaaaa
     6241 cagcagttag cgaaaattaa tagaattata ttcgcaagac aattgtgaca ttaaattaaa
     6301 aaattgtaga atttttacta tcctctttaa cgccatgagc ctttataaaa aggttaaatt
     6361 agttttaaca ttcttttttt gagtaagagt ttacaattta tcaaaacctg tgttattata
     6421 ttcattagtt tcaatttatt agcatctaga gaaaaatcaa ttggcagtta cattgttgga
     6481 atttatgaag aaaaagaatc tacaacggag aataagttgc tgatcgcttt ccctaaaatt
     6541 gtatattttg ctgagcttat tttgacattt cgttgaagtt ttctctaatt cgcattcatt
     6601 ttaagtaaaa caatgagaaa taaaattaca aaaaatacaa attaaaatac aatttttagc
     6661 tataattata gacgatgccc ttgtatccca ttctgtctcg cttgccccta ctttttatct
     6721 tttatatacc ataatgaacg ctgccgctac taaccatacc ccgattttac atttcggact
     6781 cccaaggacg tacaaaatag aaaactatag aaaaaaataa tcagaaaata gcatgtcatc
     6841 tctttgtaaa acgcgtttgc aagaagaaag gaaacaatgg agaagagatc atccatttgt
     6901 atgtaaattt tagtaaactt gaagaaatca ctaacaactt ctcttactta gggattctat
     6961 gcaaaacctt gtaaatcatc tgatggagga ctcgatttaa tgaattggaa ggttgggccc
     7021 gcggccgctt agctgaaact tccaaccctc ctgctcccgt cacattactc gacaaagcta
     7081 atgttttggc aacttctcgt ctttggcgca agactgtagc taagattttt aaggaggaat
     7141 atcctcacct taccttaaaa aaccagctca ttgactccgc agccatgctt ttggtcaaga
     7201 gccctcgtac acttaacggt gttgttttga ctgacaactt gtttggtgac attatttcag
     7261 atgaggcttc tgtcattcct ggtagcttgg gccttttgcc ttctgcctcc ctttccggtg
     7321 tggtaggaaa atcagaagaa aaggttcatt gtttggttga gcccattcac ggtagcgctc
     7381 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7441 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7501 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     7561 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     7621 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7681 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7801 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     7861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7921 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     7981 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     8041 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     8101 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     8161 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8221 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     8281 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     8341 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8401 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8461 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8521 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8581 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8641 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8701 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8761 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8821 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8881 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8941 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9001 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9061 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9121 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9181 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9241 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9301 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9361 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9421 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9481 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9541 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9601 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9661 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9721 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9781 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9841 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9901 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9961 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10021 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10081 ggaaa
//
