LOCUS       sequence-314377-       10774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10774)
  AUTHORS   Karsi A, Lawrence ML
  TITLE     Broad host range fluorescence and bioluminescence expression vectors
            for Gram-negative bacteria.
  JOURNAL   Plasmid. 2007 May;57(3):286-95. doi: 10.1016/j.plasmid.2006.11.002
  PUBMED    17207855
REFERENCE   2  (bases 1 to 10774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2006.11"; journalName: "Plasmid"; date: 
            "2007-05"; volume: "57"; issue: "3"; pages: "286-95"
COMMENT     Sequence Label: pAKlux2.1
FEATURES             Location/Qualifiers
     source          1..10774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     183..199
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     184..200
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             238..1680
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1693..2616
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2665..3747
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3762..4745
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4924..6036
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     primer_bind     complement(6054..6070)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6054..6070)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6102..6121)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6103..6121)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6131..6148)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6131..6147)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6140..6162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(6856..7518)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      7519..8290
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(join(9615..10406,10407..10475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10238..10257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10476..10547)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctgggta ccgggccccc
      181 cctcgaggtc gacggtatcg ataagcttga tatcgaattc aggcttggag gatacgtatg
      241 actaaaaaaa tttcattcat tattaacggc caggttgaaa tctttcccga aggtgatgat
      301 ttagtgcaat ccattaattt tggtgataat agtgtttacc tgccaatatt gaatgactct
      361 catgtaaaaa acattattga ttgtaatgga aataacgaat tacggttgca taacattgtc
      421 aattttctct atacggtagg gcaaagatgg aaaaatgaag aatactcaag acgcaggaca
      481 tacattcgtg acttaaaaaa atatatggga tattcagaag aaatggctaa gctagaggcc
      541 aattggatat ctatgatttt atgttctaaa ggcggccttt atgatgttgt agaaaatgaa
      601 cttggttctc gccatatcat ggatgaatgg ctacctcagg atgaaagtta tgttcgggct
      661 tttccgaaag gtaaatctgt acatctgttg gcaggtaatg ttccattatc tgggatcatg
      721 tctatattac gcgcaatttt aactaagaat cagtgtatta taaaaacatc gtcaaccgat
      781 ccttttaccg ctaatgcatt agcgttaagt tttattgatg tagaccctaa tcatccgata
      841 acgcgctctt tatctgttat atattggccc caccaaggtg atacatcact cgcaaaagaa
      901 attatgcaac atgcggatgt tattgtcgct tggggagggc cagatgcgat taattgggcg
      961 gtagagcatg cgccatctta tgctgatgtg attaaatttg gttctaaaaa gagtctttgc
     1021 attatcgata atcctgttga tttgacgtcc gcagcgacag gtgcggctca tgatgtttgt
     1081 ttttacgatc agcgagcttg tttttctgcc caaaacatat attacatggg aaatcattat
     1141 gaggaattta agttagcgtt gatagaaaaa cttaatctat atgcgcatat attaccgaat
     1201 gccaaaaaag attttgatga aaaggcggcc tattctttag ttcaaaaaga aagcttgttt
     1261 gctggattaa aagtagaggt ggatattcat caacgttgga tgattattga gtcaaatgca
     1321 ggtgtggaat ttaatcaacc acttggcaga tgtgtgtacc ttcatcacgt cgataatatt
     1381 gagcaaatat tgccttatgt tcaaaaaaat aagacgcaaa ccatatctat ttttccttgg
     1441 gagtcatcat ttaaatatcg agatgcgtta gcattaaaag gtgcggaaag gattgtagaa
     1501 gcaggaatga ataacatatt tcgagttggt ggatctcatg acggaatgag accgttgcaa
     1561 cgattagtga catatatttc tcatgaaagg ccatctaact atacggctaa ggatgttgcg
     1621 gttgaaatag aacagactcg attcctggaa gaagataagt tccttgtatt tgtcccataa
     1681 taggtaaaaa gtatggaaaa tgaatcaaaa tataaaacca tcgaccacgt tatttgtgtt
     1741 gaaggaaata aaaaaattca tgtttgggaa acgctgccag aagaaaacag cccaaagaga
     1801 aagaatgcca ttattattgc gtctggtttt gcccgcagga tggatcattt tgctggtctg
     1861 gcggaatatt tatcgcggaa tggatttcat gtgatccgct atgattcgct tcaccacgtt
     1921 ggattgagtt cagggacaat tgatgaattt acaatgtcta taggaaagca gagcttgtta
     1981 gcagtggttg attggttaac tacacgaaaa ataaataact tcggtatgtt ggcttcaagc
     2041 ttatctgcgc ggatagctta tgcaagccta tctgaaatca atgcttcgtt tttaatcacc
     2101 gcagtcggtg ttgttaactt aagatattct cttgaaagag ctttagggtt tgattatctc
     2161 agtctaccca ttaatgaatt gccgaataat ctagattttg aaggccataa attgggtgct
     2221 gaagtctttg cgagagattg tcttgatttt ggttgggaag atttagcttc tacaattaat
     2281 aacatgatgt atcttgatat accgtttatt gcttttactg caaataacga taattgggtc
     2341 aagcaagatg aagttatcac attgttatca aatattcgta gtaatcgatg caagatatat
     2401 tctttgttag gaagttcgca tgacttgagt gaaaatttag tggtcctgcg caatttttat
     2461 caatcggtta cgaaagccgc tatcgcgatg gataatgatc atctggatat tgatgttgat
     2521 attactgaac cgtcatttga acatttaact attgcgacag tcaatgaacg ccgaatgaga
     2581 attgagattg aaaatcaagc aatttctctg tcttaaaatc tattgagata ttctatcact
     2641 caaatagcaa tataaggact ctctatgaaa tttggaaact ttttgcttac ataccaacct
     2701 ccccaatttt ctcaaacaga ggtaatgaaa cgtttggtta aattaggtcg catctctgag
     2761 gagtgtggtt ttgataccgt atggttactg gagcatcatt tcacggagtt tggtttgctt
     2821 ggtaaccctt atgtcgctgc tgcatattta cttggcgcga ctaaaaaatt gaatgtagga
     2881 actgccgcta ttgttcttcc cacagcccat ccagtacgcc aacttgaaga tgtgaattta
     2941 ttggatcaaa tgtcaaaagg acgatttcgg tttggtattt gccgagggct ttacaacaag
     3001 gactttcgcg tattcggcac agatatgaat aacagtcgcg ccttagcgga atgctggtac
     3061 gggctgataa agaatggcat gacagaggga tatatggaag ctgataatga acatatcaag
     3121 ttccataagg taaaagtaaa ccccgcggcg tatagcagag gtggcgcacc ggtttatgtg
     3181 gtggctgaat cagcttcgac gactgagtgg gctgctcaat ttggcctacc gatgatatta
     3241 agttggatta taaatactaa cgaaaagaaa gcacaacttg agctttataa tgaagtggct
     3301 caagaatatg ggcacgatat tcataatatc gaccattgct tatcatatat aacatctgta
     3361 gatcatgact caattaaagc gaaagagatt tgccggaaat ttctggggca ttggtatgat
     3421 tcttatgtga atgctacgac tatttttgat gattcagacc aaacaagagg ttatgatttc
     3481 aataaagggc agtggcgtga ctttgtatta aaaggacata aagatactaa tcgccgtatt
     3541 gattacagtt acgaaatcaa tcccgtggga acgccgcagg aatgtattga cataattcaa
     3601 aaagacattg atgctacagg aatatcaaat atttgttgtg gatttgaagc taatggaaca
     3661 gtagacgaaa ttattgcttc catgaagctc ttccagtctg atgtcatgcc atttcttaaa
     3721 gaaaaacaac gttcgctatt atattagcta aggagaaaga aatgaaattt ggattgttct
     3781 tccttaactt catcaattca acaactgttc aagaacaaag tatagttcgc atgcaggaaa
     3841 taacggagta tgttgataag ttgaattttg aacagatttt agtgtatgaa aatcattttt
     3901 cagataatgg tgttgtcggc gctcctctga ctgtttctgg ttttctgctc ggtttaacag
     3961 agaaaattaa aattggttca ttaaatcaca tcattacaac tcatcatcct gtccgcatag
     4021 cggaggaagc ttgcttattg gatcagttaa gtgaagggag atttatttta gggtttagtg
     4081 attgcgaaaa aaaagatgaa atgcattttt ttaatcgccc ggttgaatat caacagcaac
     4141 tatttgaaga gtgttatgaa atcattaacg atgctttaac aacaggctat tgtaatccag
     4201 ataacgattt ttatagcttc cctaaaatat ctgtaaatcc ccatgcttat acgccaggcg
     4261 gacctcggaa atatgtaaca gcaaccagtc atcatattgt tgagtgggcg gccaaaaaag
     4321 gtattcctct catctttaag tgggatgatt ctaatgatgt tagatatgaa tatgctgaaa
     4381 gatataaagc cgttgcggat aaatatgacg ttgacctatc agagatagac catcagttaa
     4441 tgatattagt taactataac gaagatagta ataaagctaa acaagagacg cgtgcattta
     4501 ttagtgatta tgttcttgaa atgcacccta atgaaaattt cgaaaataaa cttgaagaaa
     4561 taattgcaga aaacgctgtc ggaaattata cggagtgtat aactgcggct aagttggcaa
     4621 ttgaaaagtg tggtgcgaaa agtgtattgc tgtcctttga accaatgaat gatttgatga
     4681 gccaaaaaaa tgtaatcaat attgttgatg ataatattaa gaagtaccac atggaatata
     4741 cctaatagat ttcgagttgc agcgaggcgg caagtgaacg aatccccagg agcatagata
     4801 actatgtgac tggggtgagt gaaagcagcc aacaaagcag cagcttgaaa gatgaagggt
     4861 ataaaagagt atgacagcag tgctgccata ctttctaata ttatcttgag gagtaaaaca
     4921 ggtatgactt catatgttga taaacaagaa attacagcaa gctcagaaat tgatgatttg
     4981 attttttcga gcgatccatt agtgtggtct tacgacgagc aggaaaaaat cagaaagaaa
     5041 cttgtgcttg atgcatttcg taatcattat aaacattgtc gagaatatcg tcactactgt
     5101 caggcacaca aagtagatga caatattacg gaaattgatg acatacctgt attcccaaca
     5161 tcggttttta agtttactcg cttattaact tctcaggaaa acgagattga aagttggttt
     5221 accagtagcg gcacgaatgg tttaaaaagt caggtggcgc gtgacagatt aagtattgag
     5281 agactcttag gctctgtgag ttatggcatg aaatatgttg gtagttggtt tgatcatcaa
     5341 atagaattag tcaatttggg accagataga tttaatgctc ataatatttg gtttaaatat
     5401 gttatgagtt tggtggaatt gttatatcct acgacattta ccgtaacaga agaacgaata
     5461 gattttgtta aaacattgaa tagtcttgaa cgaataaaaa atcaagggaa agatctttgt
     5521 cttattggtt cgccatactt tatttattta ctctgccatt atatgaaaga taaaaaaatc
     5581 tcattttctg gagataaaag cctttatatc ataaccggag gcggctggaa aagttacgaa
     5641 aaagaatctc tgaaacgtga tgatttcaat catcttttat ttgatacttt caatctcagt
     5701 gatattagtc agatccgaga tatatttaat caagttgaac tcaacacttg tttctttgag
     5761 gatgaaatgc agcgtaaaca tgttccgccg tgggtatatg cgcgagcgct tgatcctgaa
     5821 acgttgaaac ctgtacctga tggaacgccg gggttgatga gttatatgga tgcgtcagca
     5881 accagttatc cagcatttat tgttaccgat gatgtcggga taattagcag agaatatggt
     5941 aagtatcccg gcgtgctcgt tgaaatttta cgtcgcgtca atacgaggac gcagaaaggg
     6001 tgtgctttaa gcttaaccga agcgtttgat agttgatgac ctgcagcccg ggggatccac
     6061 tagttctaga gcggccgcca ccgcggtgga gctccaattc gccctatagt gagtcgtatt
     6121 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6181 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6241 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
     6301 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     6361 ggccgtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc
     6421 cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat ggcagaaatt
     6481 cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt cgttaaatag ccgcttatgt
     6541 ctattgctgg tttaccggtt tattgactac cggaagcagt gtgaccgtgt gcttctcaaa
     6601 tgcctgaggc cagtttgctc aggctctccc cgtggaggta ataattgacg atatgatcat
     6661 ttattctgcc tcccagagcc tgataaaaac ggtgaatccg ttagcgaggt gccgccggct
     6721 tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca
     6781 aggtataggg cggcgaggcg gctacagccg atagtctgga acagcgcact tacgggttgc
     6841 tgcgcaaccc aagtgctacc ggcgcggcag cgtgacccgt gtcggcggct ccaacggctc
     6901 gccatcgtcc agaaaacacg gctcatcggg catcggcagg cgctgctgcc cgcgccgttc
     6961 ccattcctcc gtttcggtca aggctggcag gtctggttcc atgcccggaa tgccgggctg
     7021 gctgggcggc tcctcgccgg ggccggtcgg tagttgctgc tcgcccggat acagggtcgg
     7081 gatgcggcgc aggtcgccat gccccaacag cgattcgtcc tggtcgtcgt gatcaaccac
     7141 cacggcggca ctgaacaccg acaggcgcaa ctggtcgcgg ggctggcccc acgccacgcg
     7201 gtcattgacc acgtaggccg acacggtgcc ggggccgttg agcttcacga cggagatcca
     7261 gcgctcggcc accaagtcct tgactgcgta ttggaccgtc cgcaaagaac gtccgatgag
     7321 cttggaaagt gtcttctggc tgaccaccac ggcgttctgg tggcccatct gcgccacgag
     7381 gtgatgcagc agcattgccg ccgtgggttt cctcgcaata agcccggccc acgcctcatg
     7441 cgctttgcgt tccgtttgca cccagtgacc gggcttgttc ttggcttgaa tgccgatttc
     7501 tctggactgc gtggccatgc ttatctccat gcggtagggg tgccgcacgg ttgcggcacc
     7561 atgcgcaatc agctgcaact tttcggcagc gcgacaacaa ttatgcgttg cgtaaaagtg
     7621 gcagtcaatt acagattttc tttaacctac gcaatgagct attgcggggg gtgccgcaat
     7681 gagctgttgc gtacccccct tttttaagtt gttgattttt aagtctttcg catttcgccc
     7741 tatatctagt tctttggtgc ccaaagaagg gcacccctgc ggggttcccc cacgccttcg
     7801 gcgcggctcc ccctccggca aaaagtggcc cctccggggc ttgttgatcg actgcgcggc
     7861 cttcggcctt gcccaaggtg gcgctgcccc cttggaaccc ccgcactcgc cgccgtgagg
     7921 ctcggggggc aggcgggcgg gcttcgccct tcgactgccc ccactcgcat aggcttgggt
     7981 cgttccaggc gcgtcaaggc caagccgctg cgcggtcgct gcgcgagcct tgacccgcct
     8041 tccacttggt gtccaaccgg caagcgaagc gcgcaggccg caggccggag gcttttcccc
     8101 agagaaaatt aaaaaaattg atggggcaag gccgcaggcc gcgcagttgg agccggtggg
     8161 tatgtggtcg aaggctgggt agccggtggg caatccctgt ggtcaagctc gtgggcaggc
     8221 gcagcctgtc catcagcttg tccagcaggg ttgtccacgg gccgagcgaa gcgagccagc
     8281 cggtggccgc tcgcggccat cgtccacata tccacgggct ggcaagggag cgcagcgacc
     8341 gcgcagggcg aagcccggag agcaagcccg tagggcgccg cagccgccgt aggcggtcac
     8401 gactttgcga agcaaagtct agtgagtata ctcaagcatt gagtggcccg ccggaggcac
     8461 cgccttgcgc tgcccccgtc gagccggttg gacaccaaaa gggaggggca ggcatggcgg
     8521 catacgcgat catgcgatgc aagaagctgg cgaaaatggg caacgtggcg gccagtctca
     8581 agcacgccta ccgcgagcgc gagacgccca acgctgacgc cagcaggacg ccagagaacg
     8641 agcactgggc ggccagcagc accgatgaag cgatgggccg actgcgcgag ttgctgccag
     8701 agaagcggcg caaggacgct gtgttggcgg tcgagtacgt catgacggcc agcccggaat
     8761 ggtggaagtc ggccagccaa gaacagcagg cggcgttctt cgagaaggcg cacaagtggc
     8821 tggcggacaa gtacggggcg gatcgcatcg tgacggccag catccaccgt gacgaaacca
     8881 gcccgcacat gaccgcgttc gtggtgccgc tgacgcagga cggcaggctg tcggccaagg
     8941 agttcatcgg caacaaagcg cagatgaccc gcgaccagac cacgtttgcg gccgctgtgg
     9001 ccgatctagg gctgcaacgg ggcatcgagg gcagcaaggc acgtcacacg cgcattcagg
     9061 cgttctacga ggccctggag cggccaccag tgggccacgt caccatcagc ccgcaagcgg
     9121 tcgagccacg cgcctatgca ccgcagggat tggccgaaaa gctgggaatc tcaaagcgcg
     9181 ttgagacgcc ggaagccgtg gccgaccggc tgacaaaagc ggttcggcag gggtatgagc
     9241 ctgccctaca ggccgccgca ggagcgcgtg agatgcgcaa gaaggccgat caagcccaag
     9301 agacggcccg agaccttcgg gagcgcctga agcccgttct ggacgccctg gggccgttga
     9361 atcgggatat gcaggccaag gccgccgcga tcatcaaggc cgtgggcgaa aagctgctga
     9421 cggaacagcg ggaagtccag cgccagaaac aggcccagcg ccagcaggaa cgcgggcgcg
     9481 cacatttccc cgaaaagtgc cacctggatt ttggtcatga gattatcaaa aaggatcttc
     9541 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9601 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9661 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9721 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9781 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9841 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9901 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9961 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10021 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10081 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10141 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10201 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10261 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10321 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10381 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10441 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10501 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgggc aaatattata
    10561 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
    10621 gcttccatgt cggcagaatg cttaatgaat tacaacagtt tttatgcatg cgcccaatac
    10681 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    10741 ccgactggaa agcgggcagt gagcgcaacg caat
//
