LOCUS       sequence-314393-        5056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing Ad5 Fiber gene flanked by homology arms for 
            insertion into the recombinant Ad5 vector IQ013 to generate tropism 
            modified vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, 
            Newgard CB
  TITLE     Creation of versatile cloning platforms for transgene expression and
            dCas9-based epigenome editing.
  JOURNAL   Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 
            10.1093/nar/gky1286.
  PUBMED    30590691
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286."
COMMENT     Sequence Label: IX001: pBS/Fiber
FEATURES             Location/Qualifiers
     source          1..5056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             50..76
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     99..119
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     5'UTR           2163..2205
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     complement(2242..2262)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2242..2260)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2281..2297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2281..2297)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2294..2316)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2305..2321
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2329..2335,2336..2353,2354..2359))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2374..2395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2512..2529)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2683..3271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2763..2782)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3442..4233,4234..4302))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4065..4084
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4303..4407)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4434..4890
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4521..4540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4731..4752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5016..5038
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5030..5047
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5031..5047
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggt acgacccaag caggctagtt acccatacga
       61 tgtgccagat tacgctggag gcagatcaca cctaagcacg caaatgggcg gtaggcgtgc
      121 gtctcacagc agcacctcct tgccctcctc ccacgtcgac ggctctggta ttgcagcttc
      181 ctcctggctg caaactttct ccacaatcta aatggaatgt cagtttcctc ctgttcctgt
      241 ccatccgcac ccactatctt catgttgttg cagatgaagc gcgcaagacc gtctgaagat
      301 accttcaacc ccgtgtatcc atatgacacg gaaaccggtc ctccaactgt gccttttctt
      361 actcctccct ttgtatcccc caatgggttt caagagagtc cccctggggt actctctttg
      421 cgcctatccg aacctctagt tacctccaat ggcatgcttg cgctcaaaat gggcaacggc
      481 ctctctctgg acgaggccgg caaccttacc tcccaaaatg taaccactgt gagcccacct
      541 ctcaaaaaaa ccaagtcaaa cataaacctg gaaatatctg cacccctcac agttacctca
      601 gaagccctaa ctgtggctgc cgccgcacct ctaatggtcg cgggcaacac actcaccatg
      661 caatcacagg ccccgctaac cgtgcacgac tccaaactta gcattgccac ccaaggaccc
      721 ctcacagtgt cagaaggaaa gctagccctg caaacatcag gccccctcac caccaccgat
      781 agcagtaccc ttactatcac tgcctcaccc cctctaacta ctgccactgg tagcttgggc
      841 attgacttga aagagcccat ttatacacaa aatggaaaac taggactaaa gtacggggct
      901 cctttgcatg taacagacga cctaaacact ttgaccgtag caactggtcc aggtgtgact
      961 attaataata cttccttgca aactaaagtt actggagcct tgggttttga ttcacaaggc
     1021 aatatgcaac ttaatgtagc aggaggacta aggattgatt ctcaaaacag acgccttata
     1081 cttgatgtta gttatccgtt tgatgctcaa aaccaactaa atctaagact aggacagggc
     1141 cctcttttta taaactcagc ccacaacttg gatattaact acaacaaagg cctttacttg
     1201 tttacagctt caaacaattc caaaaagctt gaggttaacc taagcactgc caaggggttg
     1261 atgtttgacg ctacagccat agccattaat gcaggagatg ggcttgaatt tggttcacct
     1321 aatgcaccaa acacaaatcc cctcaaaaca aaaattggcc atggcctaga atttgattca
     1381 aacaaggcta tggttcctaa actaggaact ggccttagtt ttgacagcac aggtgccatt
     1441 acagtaggaa acaaaaataa tgataagcta actttgtgga ccacaccagc tccatctcct
     1501 aactgtagac taaatgcaga gaaagatgct aaactcactt tggtcttaac aaaatgtggc
     1561 agtcaaatac ttgctacagt ttcagttttg gctgttaaag gcagtttggc tccaatatct
     1621 ggaacagttc aaagtgctca tcttattata agatttgacg aaaatggagt gctactaaac
     1681 aattccttcc tggacccaga atattggaac tttagaaatg gagatcttac tgaaggcaca
     1741 gcctatacaa acgctgttgg atttatgcct aacctatcag cttatccaaa atctcacggt
     1801 aaaactgcca aaagtaacat tgtcagtcaa gtttacttaa acggagacaa aactaaacct
     1861 gtaacactaa ccattacact aaacggtaca caggaaacag gagacacaac tccaagtgca
     1921 tactctatgt cattttcatg ggactggtct ggccacaact acattaatga aatatttgcc
     1981 acatcctctt acactttttc atacattgcc caagaataaa gaatcgtttg tgttatgttt
     2041 caacgtgttt atttttcaat tgcagaaaat ttcgaatcat ttttcattca gtagtatagc
     2101 cccagagacg ggtaagccta tccctaaccc tctcctcggt ctcgattgta gaatacgagc
     2161 ttcttgttct ttttgcagaa gcacagaata aacgctcaac tttggcagct gtgaacattc
     2221 tacgagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     2281 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2341 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2401 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2461 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2521 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2581 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2641 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2701 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2761 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2821 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2881 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2941 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3001 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3061 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3121 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3181 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3241 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3301 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3361 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3421 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3481 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3541 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3601 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3661 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3721 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3781 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3841 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3901 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3961 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4021 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4081 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4141 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4201 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4261 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4321 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4381 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
     4441 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4501 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4561 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4621 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4681 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4741 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4801 tttgccgatt tcggcctatt ggttaaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4861 attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg
     4921 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     4981 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     5041 ggccagtgag cgcgcg
//
