LOCUS       sequence-314410-        6511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N1199 narnavirus cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6511)
  TITLE     Yeast viruses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAG25-1199 cDNA
FEATURES             Location/Qualifiers
     source          1..6511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..1120
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        1..344
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             345..917
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      923..1120
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(4034..4053)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4035..4053)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4140..4157)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4311..4899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4391..4410)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5070..5861,5862..5930))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5693..5712
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5931..6035)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6103..6121
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6159..6181)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6281..6300
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6381..6399
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6381..6398
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
       61 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
      121 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag
      181 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
      241 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
      301 ttgctaggat acagttctca catcacatcc gaacataaac aaccatgggt accactcttg
      361 acgacacggc ttaccggtac cgcaccagtg tcccggggga cgccgaggcc atcgaggcac
      421 tggatgggtc cttcaccacc gacaccgtct tccgcgtcac cgccaccggg gacggcttca
      481 ccctgcggga ggtgccggtg gacccgcccc tgaccaaggt gttccccgac gacgaatcgg
      541 acgacgaatc ggacgacggg gaggacggcg acccggactc ccggacgttc gtcgcgtacg
      601 gggacgacgg cgacctggcg ggcttcgtgg tcgtctcgta ctccggctgg aaccgccggc
      661 tgaccgtcga ggacatcgag gtcgccccgg agcaccgggg gcacggggtc gggcgcgcgt
      721 tgatggggct cgcgacggag ttcgcccgcg agcggggcgc cgggcacctc tggctggagg
      781 tcaccaacgt caacgcaccg gcgatccacg cgtaccggcg gatggggttc accctctgcg
      841 gcctggacac cgccctgtac gacggcaccg cctcggacgg cgagcaggcg ctctacatga
      901 gcatgccctg cccctaatca gtactgacaa taaaaagatt cttgttttca agaacttgtc
      961 atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta
     1021 tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat
     1081 atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg
     1141 ccgccatcca gtgtcgaaaa cgagctcgaa ttcatcgatg atccgaattc ccgggatccg
     1201 cgatgccatc ttccgataac accgtgttga ctgtacccgg tggggttaca gccaagatgg
     1261 acgtcaggag aggcaccgtc cccgctgcaa ttggcagcga ggcagcgcac actcctgagc
     1321 ctacttgtgc ccgcggtaac cgccgcgcgc gtgtaggtct tagggggcgt gtgcaggaga
     1381 gccctagttc ctcctcacag aggttggaca tgctccttag tgcgtgggct gcaaccgttg
     1441 cagcaaccac gaaggagcgt gtcgacacta gggtaaggag ccgtaagaac cccactcccg
     1501 ctacctcttg gtaccgggat ggggagaagt ggatccgcaa ggtctcgcgt cactccggca
     1561 tagaagctgc attagcccta tgcaaggagt tcgcaggtag cgctcggtcc gcctggatat
     1621 tcaacaagcc attctttcac catcgtctga tgaagaaggc tccattgcgt atccggcgct
     1681 ccgcaaatgc gtgggcacag ctctcctatt tagggagggc cttgccccgt gggggttctt
     1741 tggcagtctc gcgtgcgctt cgcgtgcact acgactgtct cacctccacc ttccattcgc
     1801 ggcccgacga tctttcctcg cttgaggagt ggaccgtcct gtgggcccgt cgctacctgg
     1861 accgccaccc ttcgattgcc caaacatgtt ccgtccctac tagtgggtcg gcgtgttatg
     1921 aggtctctcg gaaggatggt ggctttgatc ttgcggttag tggactcctt caagagtcga
     1981 gcgagctaga cctcgagcgt cccgatgggg tctcagacag tgtctggacc ccccgggtag
     2041 ccgagttacg tctgctcaac gtctcgctct cgaaggcccg tgaggatcgc gaggcatccc
     2101 gcccccctag ggggagggtt gtcgcgctcc ctgagcgtgg attcaaggtc cgggttgtga
     2161 ctgcaatgca ggctcacaac cttgtccttg gccacacggc ccggcgtcgc ctgatgaaag
     2221 gcatgcgccg ttggccgatg ctcagggaga ctctcgcggg caatgagtcc actgttgtgc
     2281 aggagctggt tggtggcgcc ggggaagtag tcagttcgga tcttaagtcc gcatctgacc
     2341 ttattccctt cgacgtcgtc gaggcaatct tgcgcgggct tgcacgctcc gggagacttg
     2401 atccagtcga actggagggt ctccgggtgt gtacgggccc gcagcaccta tcctggccag
     2461 atggacgaga agcagtcaca agtcgtggca tcctcatggg attgccaaca acttgggcgc
     2521 tcctcaacct ctaccatggt tggtgctggc aggttgccat cgaccacgcg agggagtatt
     2581 ctcttccttc gggccatccc gtgagggcca tcggccgcat ttgcggcgac gacctcatcg
     2641 gtaccgctcc ccggcacctg gtagactgct atgaacagcg tcttcggggt accggtgccg
     2701 tcctttcgga tggcaagcac ttccgctctt ccaatcgtgg ggtcttcctc gaggttcttt
     2761 gggagctgag ggggaaacgt ttcaccgtct atgatggtgg cgtccccatt gtccgcaagt
     2821 ctcgcgggag atgcggacgc cgtgttacgc gtcctgttaa tcaggtcgcg ttgcaccatt
     2881 gggaggtcgc gcttaagtgc gagacgttcc caataaagtc ccttgttgtg ggcgacccca
     2941 acctggctgt gccagagtat tggggtgtgt ccacaactga ggatgctcta ttgggctcct
     3001 atgatgtgcg cacggtgtct gctgtggcga ggaccgtccg tccggatctt ccatcgaagt
     3061 tccgggccgc cgggatccca ccacatctcc cacggagtct tggtggcgcg ggccttgccc
     3121 gctggcctcc aaatccgtct gagactgtgg acgcctctcg ttcacaccgg aaggcattgg
     3181 ctaccgcgct ttacggcgtc ggtgactacg tctccgcgac tgcttttggg cagtgctggc
     3241 gggacggaga accttcgcca tggcgcgaga tggccaccca ggacgtccga ttttggatcg
     3301 accaggtgtc cgtcgtagca ccttccggaa cggtcccggc ggtgggcatg gtctcactcg
     3361 gtgaccctgc acgggctttg cagtccctta tagggacaca gagccagcag tatgtcgcca
     3421 tgttggggcc agacctatcg gcacggaagt acccctccct taggaggttg gctgcttcca
     3481 tgcgccgggt ccgcgcctcc ctccgtaatc ggtggaagag cgcgaacccc gtccggaaac
     3541 cgttgagtga attgctcctt cgccagcgtt ccttatccca aggctttgag ctttgggtct
     3601 cggagtacac tggcggaagc ctcgattccc ctccgggaga ggcataccaa acggcatacc
     3661 tatcctgtag gcctcacgac tctactctcc attcctggag gcaatttgcc attgcctccc
     3721 tatccaggtt ggaccaggaa accgaccttg gccggctact tggtttcacc ctggacacct
     3781 cggtcccctc agcgtggatc ccgggaattc ggccgaattc ccgggatcct gaacctgtca
     3841 attgctggtg aattggcggc catatctacc agaaagcacc gtttcccgtc cgatcaactg
     3901 tagttaagct ggtaagagcc tgaccgagta gtgtagtggg tgaccatacg cgaaactcag
     3961 gtgctgcaat ctggatcccg ggaattcgga tcagatccac tagtggccta tgcggccgcg
     4021 gatctgccgg tctccctata gtgagtcgta ttaatttcga taagccaggt taacctgcat
     4081 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4141 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4201 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4261 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4321 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4381 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4441 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4501 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4561 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4621 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4681 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4741 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4801 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4861 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4921 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4981 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5041 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5101 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5161 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5221 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5281 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5341 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5401 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5461 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5521 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5581 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5641 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5701 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5761 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5821 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5881 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5941 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6001 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6061 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     6121 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6181 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6241 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     6301 ctgagagtgc accatatgga catattgtcg ttagaacgcg gctacaatta atacataacc
     6361 ttatgtatca tacacatacg atttaggtga cactatagaa cgcggccgcc agctgaagct
     6421 tcgtacgctg caggtcgacg gatccccggg ttaattaagg cgcgccagat ctgtttagct
     6481 tgcctcgtcc ccgccgggtc acccggccag c
//
