LOCUS       sequence-314417-        3712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non-coding DNA sequence for dinucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Davey CA
  TITLE     Near-atomic resolution structures of interdigitated nucleosome 
            fibres.
  JOURNAL   Nat Commun. 2020 Sep 21;11(1):4747. doi: 10.1038/s41467-020-18533-2.
  PUBMED    32958761
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-21"; volume: "11(1):4747. doi"; pages: " 
            10.1038/s41467-020-18533-2"
COMMENT     Sequence Label: Sticky-355-3X
FEATURES             Location/Qualifiers
     source          1..3712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1316..1333)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1316..1332)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1325..1347)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1541..1560)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1660..1682
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1720..1738)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1806..1910
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1911..1979,1980..2771)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2129..2148)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2942..3530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3431..3450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3684..3701
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
      241 taccgctgac gaaaaaaaaa acgcatcccg gtgccgaggc cgctcaattg gtcgtagaca
      301 gctctagcac cgcttaaacg cacgtacgcg ctgtctaccg cgttttaacc gccactagaa
      361 gcgcttacta gtctccaggc acgtgtgaga ccggcacatg aaaaaaaaaa tgcatgctcg
      421 agtatgaaaa aaaaaatcgc atcccggtgc cgaggccgct caattggtcg tagacagctc
      481 tagcaccgct taaacgcacg tacgcgctgt ctaccgcgtt ttaaccgcca ctagaagcgc
      541 ttactagtct ccaggcacgt gtgagaccgg cacatgaaaa aaaaaacgtc agcggtaccg
      601 ctgacgaaaa aaaaaacgca tcccggtgcc gaggccgctc aattggtcgt agacagctct
      661 agcaccgctt aaacgcacgt acgcgctgtc taccgcgttt taaccgccac tagaagcgct
      721 tactagtctc caggcacgtg tgagaccggc acatgaaaaa aaaaatgcat gctcgagtat
      781 gaaaaaaaaa atcgcatccc ggtgccgagg ccgctcaatt ggtcgtagac agctctagca
      841 ccgcttaaac gcacgtacgc gctgtctacc gcgttttaac cgccactaga agcgcttact
      901 agtctccagg cacgtgtgag accggcacat gaaaaaaaaa acgtcagcgg taccgctgac
      961 gaaaaaaaaa acgcatcccg gtgccgaggc cgctcaattg gtcgtagaca gctctagcac
     1021 cgcttaaacg cacgtacgcg ctgtctaccg cgttttaacc gccactagaa gcgcttacta
     1081 gtctccaggc acgtgtgaga ccggcacatg aaaaaaaaaa tgcatgctcg agtatgaaaa
     1141 aaaaaatcgc atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
     1201 taaacgcacg tacgcgctgt ctaccgcgtt ttaaccgcca ctagaagcgc ttactagtct
     1261 ccaggcacgt gtgagaccgg cacatgaaaa aaaaaacgtc agcggtaccg aattcactgg
     1321 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     1381 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     1441 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     1501 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     1561 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     1621 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1681 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     1741 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     1801 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1861 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1921 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1981 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2041 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2101 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2161 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2221 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2281 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2341 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2401 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     2461 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2521 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2581 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2641 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2701 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2761 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2821 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2881 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2941 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3001 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3061 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3121 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3181 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3241 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3301 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3361 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3421 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3481 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3541 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3601 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3661 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga
//
