LOCUS       sequence-314423-        4417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine Fbxw7 alpha in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar
            M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, 
            Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, 
            Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R
  TITLE     Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance 
            to PD-1 blockade.
  JOURNAL   Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416.
  PUBMED    32371478
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416."
COMMENT     Sequence Label: pENTR1A_mmFbxw7a (closed)
FEATURES             Location/Qualifiers
     source          1..4417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2646..2745)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2857..2876)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2868..3677
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2935..2954)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3767..4355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4256..4275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 tggatccggt accgaattcg cggccgcatg aatcaggaac tgctctctgt gggcagcaaa
      541 agacgacgaa ctggaggctc tctgagaggg aatgcttcct caagccaggt tgatgaggga
      601 cagatgaatc gcgtggttga ggaggatcca cagcagcaag cgagacatca agaggaggag
      661 cacactgcgc ggaatggtga acttgtgggt gcaaacccta ggcctggaga ccagaacgat
      721 acccagcaag gacaagtgga ggaaaataat aaccgcttta tttcagtaga tgaggactct
      781 tcgggaaatc aggaagagca agaggaagat gaagagcatg ctggggaaca ggaggaggaa
      841 gaggaggaag aggaagagga ggaggagatg gaccaggaga gtgatgattt tgatccgtct
      901 gatgacagta gcagagaaga tgaacatacg cacaatagca atgtcacaaa ctgcagtagt
      961 gtctcggacc tgcccgctca ccagctctcc tctccattct atacaaagac aacaaaaatg
     1021 aaaagaaagt tggaccatgg ttctgaagtt cgttcctttt ctttgggaaa gaaaccatgc
     1081 aaagtctcag attataccag taccactggc cttgtaccat gttcagcaac accaacaact
     1141 tttggggacc tgagagcagc caatgggcaa gggcagcagc ggcggaggat tacatctgtc
     1201 caaccaccca caggccttca agagtggctg aaaatgtttc agagctggag cggaccagag
     1261 aagttgctgg ctttagatga gctcattgac agctgtgaac caacacaagt gaagcatatg
     1321 atgcaagtga tagagcccca gttccagcga gacttcatct ccttgcttcc taaagagttg
     1381 gcactctatg tgctttcatt cctggaaccc aaagacctgc tgcaagcggc tcagacttgt
     1441 cgatactgga gaattttggc tgaggataac cttctctgga gagagaaatg taaagaagag
     1501 gggattgatg aaccgttgca catcaagaga agaaaaataa taaaaccagg tttcatacac
     1561 agcccatgga agagtgcgta tatcagacag cacagaattg atacaaactg gagacgagga
     1621 gaactcaaat ctcctaaggt gctgaaaggg catgatgacc atgtgatcac atgcctacag
     1681 ttttgtggca accgcatagt tagtggttct gatgacaaca ctttaaaagt ttggtcagcg
     1741 gtcacgggca agtgtctgag aacgttagtg ggacatacag gtggagtgtg gtcatcacag
     1801 atgagagaca atatcatcat cagtggatcg actgaccgga ctctcaaagt gtggaatgct
     1861 gaaactggag agtgtataca tactttatat gggcacactt ctactgtacg gtgtatgcat
     1921 ctccatgaaa aaagggttgt aagcggttct cgagatgcca ctctcagggt ttgggatatt
     1981 gagaccggcc agtgtttaca cgtcctgatg ggtcacgtag cagcggtccg ctgcgttcag
     2041 tatgatggca ggagggttgt tagtggagct tatgatttta tggtgaaggt gtgggatcca
     2101 gagactgaga cctgtctaca cacgttacag ggacacacta atagagtcta ttcattacag
     2161 tttgatggca tccatgtggt gagtggatct cttgatacat caatccgagt ctgggatgtg
     2221 gagacaggga attgtattca cacgctaaca ggacaccagt cattaacgag tggaatggaa
     2281 ctcaaagaca atattcttgt ctctgggaat gcagattcta cagttaagat ctgggatatc
     2341 aaaacaggac agtgtttaca aactttgcaa ggtcccagca agcatcagag cgctgtgacc
     2401 tgcttacagt tcaacaagaa cttcgtaatt accagctcag acgacggaac ggtcaaactc
     2461 tgggacttga aaacgggtga atttatccga aacctcgtca cattggagag tggggggagc
     2521 gggggagttg tgtggcggat cagggcctca aacacaaagc tggtgtgtgc agtcgggagt
     2581 cggaatggaa ctgaggaaac caagctcctg gtgctggact ttgatgtgga catgaaatga
     2641 tctagaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     2701 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgcagc
     2761 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2821 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     2881 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     2941 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
     3001 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     3061 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     3121 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
     3181 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     3241 ccctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     3301 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     3361 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     3421 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     3481 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     3541 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     3601 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     3661 tcgatgagtt tttctaatca gaattggtta attggttgta acattattca gattgggccc
     3721 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3781 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3841 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3901 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3961 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4021 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4081 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4141 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4201 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4261 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4321 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4381 ggttcctggc cttttgctgg ccttttgctc acatgtt
//
