LOCUS       sequence-314427-        2468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcribes E. coli SRP RNA sequence for SHAPE-Seq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Yu AM, Gasper PM, Cheng L, Lai LB, Kaur S, Gopalan V, Chen AA, Lucks
            JB
  TITLE     Computationally reconstructing cotranscriptional RNA folding from 
            experimental data reveals rearrangement of non-native folding 
            intermediates.
  JOURNAL   Mol Cell. 2021 Feb 18;81(4):870-883.e10. doi: 
            10.1016/j.molcel.2020.12.017. Epub 2021 Jan 15.
  PUBMED    33453165
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.12.017"; journalName: "Mol Cell"; date: 
            "2021-02-18- 18"; volume: "81"; issue: "4"; pages: "870-883.e10"
COMMENT     Sequence Label: pJBL3664
FEATURES             Location/Qualifiers
     source          1..2468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             570..1229
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(636..655)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1250..1344
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1751..1768)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1885..2430)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1965..1984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt
       61 gtaattctca tgtttgacag cttatcatcg ataagcttcc gatggcgcgc cgagaggctt
      121 tacactttat gcttccggct gaattctaaa gatctttgac agctagctca gtcctaggta
      181 taatgaattc atcgggggct ctgttggttc tcccgcaacg ctactctgtt taccaggtca
      241 ggtccggaag gaagcagcca aggcagatga cgcgtgtgcc gggatgtagc tggcagggcc
      301 cccacccggg tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga
      361 ggacgtcgtc cactcggatg gctaagggag agctcggatc acccagcccg cctaataagc
      421 gggctttttt ttgaacagga tccttactcg agtctagact gcagttgatc gggcacgtaa
      481 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat
      541 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
      601 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
      661 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
      721 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
      781 cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
      841 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
      901 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
      961 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     1021 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     1081 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     1141 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     1201 actgcgatga gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg actcctgttg
     1261 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
     1321 cgggcgtttt ttattggtga gaatccaagc ctccgatcaa cgtctcattt tcgccaaaag
     1381 ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga
     1441 tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg
     1501 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
     1561 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg
     1621 ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt
     1681 catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat
     1741 attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa
     1801 tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag
     1861 tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga
     1921 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1981 tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     2041 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag
     2101 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
     2161 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag
     2221 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact
     2281 gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg
     2341 gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa
     2401 gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat
     2461 ttctagat
//
