LOCUS       sequence-314452-       10349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of orthogonal tRNA and tRNA synthetase for incorporation 
            of unnatural amino acids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Jang HS, Gu X, Cooley RB, Porter JJ, Henson RL, Willi T, DiDonato 
            JA, Hazen SL, Mehl RA
  TITLE     Efficient Site-Specific Prokaryotic and Eukaryotic Incorporation of 
            Halotyrosine Amino Acids into Proteins.
  JOURNAL   ACS Chem Biol. 2020 Feb 21;15(2):562-574. doi: 
            10.1021/acschembio.9b01026. Epub 2020 Feb 10.
  PUBMED    31994864
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.9b01026"; journalName: "ACS Chem Biol"; date: 
            "2020-02-21- 21"; volume: "15"; issue: "2"; pages: "562-574"
COMMENT     Sequence Label: pAcBac1-haloTyrRS C6
FEATURES             Location/Qualifiers
     source          1..10349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      667..676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             697..723
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             727..750
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2033..2621
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2086..2106)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2504..2515)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2670..2687)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2676..2900
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2937..3185
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        3262..3356
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    3310..3328
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3338..3356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(3846..3851,3852..3868,3869..3874)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4063..4596
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  4663..4887
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(5021..5038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5192..5780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5272..5291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5951..6742,6743..6811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6574..6593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6812..6916)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6942..7396)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7079..7100)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7290..7309
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  7580..7745
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        complement(7838..7932)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    7838..7856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7866..7884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(8009..8257)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     polyA_signal    8290..8424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8314..8333)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8368..8387
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(8570..10057,10058..10105))
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     promoter        complement(10164..10255)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(10222..10239)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgcca ccatggcgtg tccggttcct ttgcagttgc ctccactgga gcgcctcaca
      721 ctcgacgact acaaggacga cgacgacaag gacaagaaac ccctggacgt gctgatcagc
      781 gccaccggcc tgtggatgag ccggaccggc accctgcaca agatcaagca ccacgaggtg
      841 tcaagaagca aaatctacat cgagatggcc tgcggcgacc acctggtggt gaacaacagc
      901 agaagctgcc ggaccgccag agccttccgg caccacaagt acagaaagac ctgtaagcgg
      961 tgccgggtgt ccgacgagga catcaacaac tttctgacca gaagcaccga gagcaagaac
     1021 agcgtgaaag tgcgggtggt gtccgccccc aaagtgaaga aagccatgcc caagagcgtg
     1081 tccagagccc ccaagcccct ggaaaacagc gtgtccgcca aggccagcac caacaccagc
     1141 cgcagcgtgc ccagccccgc caagagcacc cccaacagct ccgtgcccgc ctctgctcct
     1201 gctcccagcc tgacacggtc ccagctggac agagtggagg ccctgctgtc ccccgaggac
     1261 aagatcagcc tgaacatggc caagcccttc cgggagctgg aacccgagct ggtgacccgg
     1321 cggaagaacg acttccagcg gctgtacacc aacgaccggg aggactacct gggcaagctg
     1381 gaacgggaca tcaccaagtt cttcgtggac cggggcttcc tggaaatcaa gagccccatc
     1441 ctgatccccg ccgagtacgt ggagcggatg ggcatcaaca acgacaccga gctgtccaag
     1501 cagattttcc gggtggacaa gaacctgtgc ctgcggccta ccctggcccc cacctcactc
     1561 aactacctgc ggaaactgga cagaatcctg cctggcccca tcaagatttt cgaagtggga
     1621 ccctgctacc ggaaagagag cgacggcaaa gagcacctgg aagagtttac aatggtgggt
     1681 tttacccaga tgggcagcgg ctgcacccgg gagaacctgg aagccctgat caaagagttc
     1741 ctggattacc tggaaatcga cttcgagatc gtgggcgaca gctgcatggt gtacggcgac
     1801 accctggaca tcatgcacgg cgacctggaa ctgagcagcg ccgtggtggg acccgtgtcc
     1861 ctggaccggg agtggggcat cgacaagccc tggatcggag ccggcttcgg cctggaacgg
     1921 ctgctgaaag tgatgcacgg cttcaagaac atcaagcggg ccagcagaag cgagagctac
     1981 tacaacggca tcagcaccaa cctgtgatga gaattcaacg cgttaagtcg acaatcaacc
     2041 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     2101 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     2161 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     2221 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     2281 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     2341 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     2401 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     2461 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     2521 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     2581 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aactcgagtc
     2641 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     2701 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     2761 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     2821 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     2881 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agatctcggg caggaagagg
     2941 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     3001 ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     3061 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     3121 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     3181 acaccggaaa cctgatcatg tagatcgaac ggactctaaa tccgttcagc cgggttagat
     3241 tcccggggtt tccgtttttt aatatttgca tgtcgctatg tgttctggga aatcaccata
     3301 aacgtgaaat ccctatcagt gatagagact tataagttcc ctatcagtga tagagacacc
     3361 ggggggtgga tcgaatagat cacacggact ctaaatccgt gcaggcgggt gaaactcccg
     3421 caccccccgt tttttcagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     3481 attaagcgca agccctgcca tagccactac gggtacgtag ccaaccacta gaactatagc
     3541 tagagtcctg ggcgaacaaa cgatgctcgc cttccagaaa accgaggatg cgaaccactt
     3601 catccggggt cagcaccacc ggcaagcgcc gcgacggccg aggtctaccg atctcctgaa
     3661 gcaagggcag atccgtgcac agcaccttgc cgtagaagaa cagcaaggcc gccaatgcct
     3721 gacgatgcgt ggagaccgaa accttgcgct cgttcgccag ccaggacaga aatgcctcga
     3781 cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     3841 cccagttgac ataagcctgt tcggttcgta aactgtaatg caagtagcgt atgcgctcac
     3901 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     3961 tggcttgtta tgactgtttt tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc
     4021 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagcaac
     4081 gatgttacgc agcagggcag tcgccctaaa acaaagttag gtggctcaag tatgggcatc
     4141 attcgcacat gtaggctcgg ccctgaccaa gtcaaatcca tgcgggctgc tcttgatctt
     4201 ttcggtcgtg agttcggaga cgtagccacc tactcccaac atcagccgga ctccgattac
     4261 ctcgggaact tgctccgtag taagacattc atcgcgcttg ctgccttcga ccaagaagcg
     4321 gttgttggcg ctctcgcggc ttacgttctg cccaagtttg agcagccgcg tagtgagatc
     4381 tatatctatg atctcgcagt ctccggcgag caccggaggc agggcattgc caccgcgctc
     4441 atcaatctcc tcaagcatga ggccaacgcg cttggtgctt atgtgatcta cgtgcaagca
     4501 gattacggtg acgatcccgc agtggctctc tatacaaagt tgggcatacg ggaagaagtg
     4561 atgcactttg atatcgaccc aagtaccgcc acctaacaat tcgttcaagc cgagatcggc
     4621 ttcccggccg cggagttgtt cggtaaattg tcacaacgcc gcgaatatag tctttaccat
     4681 gcccttggcc acgcccctct ttaatacgac gggcaatttg cacttcagaa aatgaagagt
     4741 ttgctttagc cataacaaaa gtccagtatg ctttttcaca gcataactgg actgatttca
     4801 gtttacaact attctgtcta gtttaagact ttattgtcat agtttagatc tattttgttc
     4861 agtttaagac tttattgtcc gcccacaccc gcttacgcag ggcatccatt tattactcaa
     4921 ccgtaaccga ttttgccagg ttacgcggct ggtctatgcg gtgtgaaata ccgcacagat
     4981 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     5041 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6241 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6541 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6781 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6901 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     6961 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     7021 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     7081 agtttggaac aagagtccac tattaagaac gtggactcca acgtcaaagg gcgaaaaacc
     7141 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     7201 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     7261 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     7321 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     7381 ccgctacagg gcgcgtccca ttcgccattc aggctgcaaa taagcgttga tattcagtca
     7441 attacaaaca ttaataacga agagatgaca gaaaaatttt cattctgtga cagagaaaaa
     7501 gtagccgaag atgacggttt gtcacatgga gttggcagga tgtttgatta aaaacataac
     7561 aggaagaaaa atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa
     7621 tggagttttt aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg
     7681 tcgtaagcta atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc
     7741 tggttcggat ctcctagcaa aaaacggggg gtgcgggagt ttcacccgcc tgcacggatt
     7801 tagagtccgt gtgatctatt cgatccaccc cccggtgtct ctatcactga tagggaactt
     7861 ataagtctct atcactgata gggatttcac gtttatggtg atttcccaga acacatagcg
     7921 acatgcaaat attaaaaaac ggaaaccccg ggaatctaac ccggctgaac ggatttagag
     7981 tccgttcgat ctacatgatc aggtttccgg tgtttcgtcc tttccacaag atatataaag
     8041 ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt ttaaaacata
     8101 attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta ttttgtacta
     8161 atatctttgt gtttacagtc aaattaattc taattatctc tctaacagcc ttgtatcgta
     8221 tatgcaaata tgaaggaatc atgggaaata ggccctcttc ctgcccgacc taggctcaag
     8281 cagtgatcag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     8341 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     8401 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     8461 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     8521 gattatgatc ctctagtact tctcgacaag cttggccggc ctggccttat cactttccaa
     8581 gtcggttcat ctctatgtct gtataaatct gtcttttctt ggtgtgcttt aatttaatgc
     8641 aaagatggat accaactcgg agaaccaaga atagtccaat gattaaccct atgataaaga
     8701 aaaaagaggc aatagagctt ttccaactac tgaaccaacc ttctacaagc tcgattggat
     8761 ttttggatag cccagtatca ccaaaaaata aactctcatc atcaggaagt tgcgaagcag
     8821 cgtcttgaat gtgaggatgt tcgaacacct gagcctttga gctaagatga agatcggagt
     8881 ccaacatacc atgtccaatc atgtataaag gaaacttata tcctgaactg gtcctcagaa
     8941 ctccattggg tccaatttcc acgtcttcat atggtgccca gtcatcccac agttcccttt
     9001 ctgtggtagt tccactgatc attccgacca ttcttgagag gattggagca gcaatatcga
     9061 ctctgatgta tctggtctca aagtatttta gggtaccatt gattatggtg aaagcaggac
     9121 cggttcctgg gtttttagga gcaagatagc tgagatccac tggagagatt ggaagacccg
     9181 ctctgatttt gctccaggtt tcttggcaga gggaataatc caagatcctc tcaacgtcct
     9241 gaattagact tacatccact gaggtctgag atggagcaga gatacttgac ccttctgggc
     9301 attcagggaa tctggctgca gcaaagagat ccttatcagc catctcgaac cagacacctg
     9361 atgggagtct gactccccaa tgcttgcagt attgcatttt gcaggccttg cctccagttt
     9421 cataagcaaa gtagttactt ctgaaccctg tgccctcctt tcccagggat gatagctctc
     9481 cgtcctctga gaagaaggtg atgtccatgg aaatgaggtt agaatcacat agccctttga
     9541 ccttatagtc agaatgccag gttgtagagt tatggacagt ggggcatatg taattgctgc
     9601 attttccgtt gatgaactgt gaatcaaccc attctcctgt gtattcatca accagcacat
     9661 ggtgaggagt cacctggaca atcactgctt cggcatccgt cacagttgca tatccacaac
     9721 tttgaggagg gaagcctgga ttcagccaag ttccttgttt cgtttgttca atgctttcct
     9781 tgcattgttc tacagatgga gtgaaggatc ggatggaatg tgttatatac ttcggtccat
     9841 accagcggaa atcacaagta gtgacccatt tggaagcatg acacatccaa ccgtctgctt
     9901 gaatagcctt gtgactcttg ggcattttga cttgtaaggc tgtgcctatt aagtcattat
     9961 gccaatttaa atctgagctt gacgggcaat aatggtaatt agaaggaaca tttttccagt
    10021 ttcctttttg gttgtgtgga aaaactatgg tgaacttgca attcacccca atgaataaaa
    10081 aggctaagta caaaaggcac ttcatggtgg cggtttttta ggagttcgcg cccgatggtg
    10141 ggacggtatg aataatccgg aatatttata ggttttttta ttacaaaact gttacgaaaa
    10201 cagtaaaata cttatttatt tgcgagatgg ttatcatttt aattatctcc atgatctatt
    10261 aatattccgg agtatacgga ccgcggccgc aaatacctgc aggatccgtt ttgcgctgct
    10321 tcgcgatgta cgggccagat atacgcgtt
//
