LOCUS       sequence-314453-       10349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of orthogonal tRNA and tRNA synthetase for incorporation 
            of unnatural amino acids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Porter JJ, Jang HS, Van Fossen EM, Nguyen DP, Willi TS, Cooley RB, 
            Mehl RA
  TITLE     Genetically Encoded Protein Tyrosine Nitration in Mammalian Cells.
  JOURNAL   ACS Chem Biol. 2019 Jun 21;14(6):1328-1336. doi: 
            10.1021/acschembio.9b00371. Epub 2019 Jun 4.
  PUBMED    31117397
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.9b00371"; journalName: "ACS Chem Biol"; date: 
            "2019-06-21- 21"; volume: "14"; issue: "6"; pages: "1328-1336"
COMMENT     Sequence Label: pAcBac1-3nitroY-A7-RS
FEATURES             Location/Qualifiers
     source          1..10349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(365..459)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    365..383
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    393..411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(536..784)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     polyA_signal    821..1045
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1034..1051
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    1100..1688
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1206..1217
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1615..1635
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2971..2994)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2998..3024)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     regulatory      3045..3054
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3073..3092)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3074..3092)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3137..3340)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3166..3186)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3341..3720
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3815..3906
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3831..3848
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(3965..4012,4013..5500)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    5646..5780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5683..5702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5737..5756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        5813..6061
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        6138..6232
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    6186..6204
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6214..6232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     mobile_element  6325..6490
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      6674..7128
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6761..6780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6970..6991
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7154..7258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7259..7327,7328..8119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7477..7496)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8290..8878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8779..8798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9032..9049
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  9183..9407
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(9474..10007)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(10196..10201,10202..10218,10219..10224))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgcccttgc
       61 ttcaggagat cggtagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg
      121 aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta
      181 gctatagttc tagtggttgg ctacgtaccc gtagtggcta tggcagggct tgcgcttaat
      241 gcgccgctac agggcgcgtg gggatacccc ctagagcccc agctgaaaaa acggggggtg
      301 cgggagtttc acccgcctgc acggatttag agtccgtgtg atctattcga tccacccccc
      361 ggtgtctcta tcactgatag ggaacttata agtctctatc actgataggg atttcacgtt
      421 tatggtgatt tcccagaaca catagcgaca tgcaaatatt aaaaaacgga aaccccggga
      481 atctaacccg gctgaacgga tttagagtcc gttcgatcta catgatcagg tttccggtgt
      541 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
      601 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
      661 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa
      721 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
      781 cctcttcctg cccgagatct ggttctttcc gcctcagaag ccatagagcc caccgcatcc
      841 ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
      901 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
      961 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca
     1021 gatggctggc aactagaagg cacagtcgag gctgatcagc gggtttaaac gggccctcta
     1081 gactcgagtt aaagtcgacg cggggaggcg gcccaaaggg agatccgact cgtctgaggg
     1141 cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc
     1201 gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca
     1261 acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac ggaattgtca
     1321 gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc
     1381 gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg
     1441 ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca
     1501 acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg
     1561 aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc
     1621 gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga
     1681 ggttgattgt cgacttaacg cgttgaattc tcatcacagg ttggtgctga tgccgttgta
     1741 gtagctctcg cttctgctgg cccgcttgat gttcttgaag ccgtgcatca ctttcagcag
     1801 ccgttccagg ccgaagccgg ctccgatcca gggcttgtcg atgccccact cccggtccag
     1861 ggacacgggt cccaccacgg cgctgctcag ttccaggtcg ccgtgcatga tgtccagggt
     1921 gtcgccgtac accatgcagc tgtcgcccac gatctcgaag tcgatttcca ggtaatccag
     1981 gaactctttg atcagggctt ccaggttctc ccgggtgcag ccgctgccca tctggttaaa
     2041 gcccaccatt gtaaactctt ccaggtgctc tttgccgtcg ctctctttcc ggtagcaggg
     2101 tcccacttcg aaaatcttga tggggccagg caggattctg tccagtttcc gcctgtagtt
     2161 gtacagggtg ggggccagca taggccgcag gcacaggttc ttgtccaccc ggaaaatctg
     2221 cttggacagc tcggtgtcgt tgttgatgcc catccgctcc acgtactcgg cggggatcag
     2281 gatggggctc ttgatttcca ggaagccccg gtccacgaag aacttggtga tgtcccgttc
     2341 cagcttgccc aggtagtcct ccctgtcgtt ggtgtacagc cgctggaagt cgttcttccg
     2401 ccgggtcacc agctcggttt ccagctcccg gaagggcttg gccatgttca ggctgatctt
     2461 gtcctcgggg gacagcaggg cctccactct gtccagctgg gaccgtgtca ggctgggagc
     2521 aggagcagag gcgggcacgg agctgttggg ggtgctcttg gcggggctgg gcacgctgcg
     2581 gctggtgttg gtgctggcct tggcggacac gctgttttcc aggggcttgg gggctctgga
     2641 cacgctcttg ggcatggctt tcttcacttt gggggcggac accacccgca ctttcacgct
     2701 gttcttgctc tcggtgcttc tggtcagaaa gttgttgatg tcctcgtcgg acacccggca
     2761 ccgcttacag gtctttctgt acttgtggtg ccggaaggct ctggcggtcc ggcagcttct
     2821 gctgttgttc accaccaggt ggtcgccgca ggccatctcg atgtagattt tgcttcttga
     2881 cacctcgtgg tgcttgatct tgtgcagggt gccggtccgg ctcatccaca ggccggtggc
     2941 gctaatcagc acgtccaggg gtttcttgtc cttgtcgtcg tcgtccttgt agtcgtcgag
     3001 tgtgaggcgc tccagtggag gcaactgcaa aggaaccgga cacgccatgg tggcgctagc
     3061 cagcttgggt ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt
     3121 ctctagttag ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
     3181 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
     3241 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     3301 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
     3361 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     3421 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     3481 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     3541 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     3601 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     3661 ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc
     3721 aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acggatcctg caggtatttg
     3781 cggccgcggt ccgtatactc cggaatatta atagatcatg gagataatta aaatgataac
     3841 catctcgcaa ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct
     3901 ataaatattc cggattattc ataccgtccc accatcgggc gcgaactcct aaaaaaccgc
     3961 caccatgaag tgccttttgt acttagcctt tttattcatt ggggtgaatt gcaagttcac
     4021 catagttttt ccacacaacc aaaaaggaaa ctggaaaaat gttccttcta attaccatta
     4081 ttgcccgtca agctcagatt taaattggca taatgactta ataggcacag ccttacaagt
     4141 caaaatgccc aagagtcaca aggctattca agcagacggt tggatgtgtc atgcttccaa
     4201 atgggtcact acttgtgatt tccgctggta tggaccgaag tatataacac attccatccg
     4261 atccttcact ccatctgtag aacaatgcaa ggaaagcatt gaacaaacga aacaaggaac
     4321 ttggctgaat ccaggcttcc ctcctcaaag ttgtggatat gcaactgtga cggatgccga
     4381 agcagtgatt gtccaggtga ctcctcacca tgtgctggtt gatgaataca caggagaatg
     4441 ggttgattca cagttcatca acggaaaatg cagcaattac atatgcccca ctgtccataa
     4501 ctctacaacc tggcattctg actataaggt caaagggcta tgtgattcta acctcatttc
     4561 catggacatc accttcttct cagaggacgg agagctatca tccctgggaa aggagggcac
     4621 agggttcaga agtaactact ttgcttatga aactggaggc aaggcctgca aaatgcaata
     4681 ctgcaagcat tggggagtca gactcccatc aggtgtctgg ttcgagatgg ctgataagga
     4741 tctctttgct gcagccagat tccctgaatg cccagaaggg tcaagtatct ctgctccatc
     4801 tcagacctca gtggatgtaa gtctaattca ggacgttgag aggatcttgg attattccct
     4861 ctgccaagaa acctggagca aaatcagagc gggtcttcca atctctccag tggatctcag
     4921 ctatcttgct cctaaaaacc caggaaccgg tcctgctttc accataatca atggtaccct
     4981 aaaatacttt gagaccagat acatcagagt cgatattgct gctccaatcc tctcaagaat
     5041 ggtcggaatg atcagtggaa ctaccacaga aagggaactg tgggatgact gggcaccata
     5101 tgaagacgtg gaaattggac ccaatggagt tctgaggacc agttcaggat ataagtttcc
     5161 tttatacatg attggacatg gtatgttgga ctccgatctt catcttagct caaaggctca
     5221 ggtgttcgaa catcctcaca ttcaagacgc tgcttcgcaa cttcctgatg atgagagttt
     5281 attttttggt gatactgggc tatccaaaaa tccaatcgag cttgtagaag gttggttcag
     5341 tagttggaaa agctctattg cctctttttt ctttatcata gggttaatca ttggactatt
     5401 cttggttctc cgagttggta tccatctttg cattaaatta aagcacacca agaaaagaca
     5461 gatttataca gacatagaga tgaaccgact tggaaagtga taaggccagg ccggccaagc
     5521 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
     5581 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     5641 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5701 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5761 tatcttatca tgtctggatc tgatcactgc ttgagcctag gtcgggcagg aagagggcct
     5821 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattag
     5881 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
     5941 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
     6001 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
     6061 cggaaacctg atcatgtaga tcgaacggac tctaaatccg ttcagccggg ttagattccc
     6121 ggggtttccg ttttttaata tttgcatgtc gctatgtgtt ctgggaaatc accataaacg
     6181 tgaaatccct atcagtgata gagacttata agttccctat cagtgataga gacaccgggg
     6241 ggtggatcga atagatcaca cggactctaa atccgtgcag gcgggtgaaa ctcccgcacc
     6301 ccccgttttt tgctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg
     6361 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     6421 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     6481 tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact
     6541 ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct
     6601 gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg
     6661 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6721 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6781 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6841 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6901 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6961 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     7021 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7081 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     7141 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7201 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7261 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7321 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7381 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7441 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7501 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7561 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7621 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7681 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7741 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7801 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7861 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7921 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7981 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8041 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8101 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8161 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8221 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8281 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8341 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8401 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8461 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8521 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8581 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8641 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8701 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8761 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8821 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8881 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8941 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     9001 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     9061 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca
     9121 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
     9181 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     9241 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     9301 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     9361 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac
     9421 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
     9481 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
     9541 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
     9601 gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc
     9661 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
     9721 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
     9781 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
     9841 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
     9901 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
     9961 ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca
    10021 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
    10081 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
    10141 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt
    10201 acgaaccgaa caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg
    10261 tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga
    10321 gcgcaaggtt tcggtctcca cgcatcgtc
//
