LOCUS       sequence-31446-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PKM2 (3GQY)
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..99
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttccctctga atattttgtt tactttaaga aggagatata ccatgggcag cagccatcat
       61 catcatcatc acagcagcgg cctggttccg cgtggtagta tgtcgaagcc ccatagtgaa
      121 gccgggactg ccttcattca gacccagcag ctgcacgcag ccatggctga cacattcctg
      181 gagcacatgt gccgcctgga cattgattca ccacccatca cagcccggaa cactggcatc
      241 atctgtacca ttggcccagc ttcccgatca gtggagacgt tgaaggagat gattaagtct
      301 ggaatgaatg tggctcgtct gaacttctct catggaactc atgagtacca tgcggagacc
      361 atcaagaatg tgcgcacagc cacggaaagc tttgcttctg accccatcct ctaccggccc
      421 gttgctgtgg ctctagacac taaaggacct gagatccgaa ctgggctcat caagggcagc
      481 ggcactgcag aggtggagct gaagaaggga gccactctca aaatcacgct ggataacgcc
      541 tacatggaaa agtgtgacga gaacatcctg tggctggact acaagaacat ctgcaaggtg
      601 gtggaagtgg gcagcaagat ctacgtggat gatgggctta tttctctcca ggtgaagcag
      661 aaaggtgccg acttcctggt gacggaggtg gaaaatggtg gctccttggg cagcaagaag
      721 ggtgtgaacc ttcctggggc tgctgtggac ttgcctgctg tgtcggagaa ggacatccag
      781 gatctgaagt ttggggtcga gcaggatgtt gatatggtgt ttgcgtcatt catccgcaag
      841 gcatctgatg tccatg
//
