LOCUS       sequence-31447-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBH26
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             374..391
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(444..462)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      458..505
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      542..901
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(629..648)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     839..860
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aattccctct gaatattttg tttactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggttc cgcgtggtag tccccacctc atcaaggtga
      121 cagtgaagac gcccaaagac aaggaggatt tctcagttac agacacatgc actatccagc
      181 agctgaagga agagatatct cagcgcttta aggcccaccc cgatcagctt gttctaatct
      241 ttgctggcaa aatcctcaag gatcctgact cactggcaca gtgtggagtg cgagatggcc
      301 tcactgtcca cctggtcatc aagaggcagc accgtgccat gggctgatga cgaagcttgc
      361 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      421 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      481 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
      541 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      601 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      661 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      721 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      781 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      841 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc tttttgattt
      901 ataagggatt ttgccgattc gctatgt
//
