LOCUS       sequence-31448-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBC44
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             631..648
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(701..719)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      715..762
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt atgcagagag cttcacgtct
      121 gaagagagag ctgcacatgt tagccacaga gccaccccca ggcatcacat gttggcaaga
      181 taaagaccaa atggatgacc tgcgagctca aatattaggt ggagccaaca caccttatga
      241 gaaaggtgtt tttaagctag aagttatcat tcctgagagg tacccatttg aacctcctca
      301 gatccgattt ctcactccaa tttatcatcc aaacattgat tctgctggaa ggatttgtct
      361 ggatgttctc aaattgccac caaaaggtgc ttggagacca tccctcaaca tcgcaactgt
      421 gttgacctct attcagctgc tcatgtcaga acccaaccct gatgacccgc tcatggctga
      481 catatcctca gaatttaaat ataataagcc agccttcctc aagaatgcca gacagtggac
      541 agagaagcat gcaagacaga aacaaaaggc tgatgaggaa gagatgcttg ataatctacc
      601 atgatgacga agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      661 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      721 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      781 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      841 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgc
//
