LOCUS       sequence-31449-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBC45
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..99
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             732..749
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(802..820)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttccctctga atattttgtt tactttaaga aggagatata ccatgggcag cagccatcat
       61 catcatcatc acagcagcgg cctggttccg cgtggtagta tggccaacat cgcggtgcag
      121 cgaatcaagc gggagttcaa ggaggtgctg aagagcgagg agacgagcaa aaatcaaatt
      181 aaagtagatc ttgtagatga gaattttaca gaattaagag gagaaatagc aggacctcca
      241 gacacaccat atgaaggagg aagataccaa ctagagataa aaataccaga aacataccca
      301 tttaatcccc ctaaggtccg gtttatcact aaaatatggc atcctaatat tagttccgtc
      361 acaggggcta tttgtttgga tatcctgaaa gatcaatggg cagctgcaat gactctccgc
      421 acggtattat tgtcattgca agcactattg gcagctgcag agccagatga tccacaggat
      481 gctgtagtag caaatcagta caaacaaaat cccgaaatgt tcaaacagac agctcgactt
      541 tgggcacatg tgtatgctgg agcaccagtt tctagtccag aatacaccaa aaaaatagaa
      601 aacctatgtg ctatgggctt tgataggaat gcagtaatag tggccttgtc ttcaaaatca
      661 tgggatgtag agactgcaac agaattgctt ctgagtaact gatgatgacg aagcttgcgg
      721 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      781 aagctgagtt ggctgctgcc accgctgagc aataactagc at
//
