LOCUS       sequence-314493-       11505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible Bxb1 integrase for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11505)
  AUTHORS   Chow KK, Budde MW, Granados AA, Cabrera M, Yoon S, Cho S, Huang TH, 
            Koulena N, Frieda KL, Cai L, Lois C, Elowitz MB
  TITLE     Imaging cell lineage with a synthetic digital recording system.
  JOURNAL   Science. 2021 Apr 9;372(6538). pii: 372/6538/eabb3099. doi: 
            10.1126/science.abb3099.
  PUBMED    33833095
REFERENCE   2  (bases 1 to 11505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2021-04-9"; volume: "372(6538). pii: 372/6538/eabb3099. doi";
            pages: " 10.1126/science.abb3099"
COMMENT     Sequence Label: TIGRE-TRE-[poor kozak]Bxb1-ecDHFR-BGHpA
FEATURES             Location/Qualifiers
     source          1..11505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    414..447
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     promoter        3011..3389
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    3019..3037
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3055..3073
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3091..3109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3127..3145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3163..3181
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3199..3217
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3235..3253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3298..3322
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4938..5414
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     polyA_signal    5447..5671
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8137..8636
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8172..8195)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8569..8588
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(9284..9300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9284..9300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9297..9319)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9308..9324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9332..9338,9339..9356,9357..9362))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9377..9398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9515..9532)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9686..10274)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9766..9785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10445..11236,11237..11305))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11068..11087
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11306..11410)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11478..11496
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattggt tttatgccgc
      481 ggttaccgtg aagatatctt aattaagaat tcgagctcgc tagagcccca tcctcactga
      541 ctccgtcctg gagttggatg agagataatg gccttacgtt gtgccagggg agggtcgggc
      601 tggatttagc aagatttacc ttctccaaag agcggtgctg cagtggcaca gctgcccacg
      661 gaggtggggg ggtcaccgtc cctggaggtg atgaagaact gtggggatgt ggcactgagg
      721 gacatggcca gtgggcacgg tgggtgggtt ggggttggtc ttggggatct tggagggctt
      781 ttccagcctt catgatttga cgattgtatg aacatctaca tggcaattct ccagctgcct
      841 gtcccagtcc tactgaccca gctgtatctc tccaggcaag ctcttccacc ccttctgctt
      901 gcatccagac accatcaaac atgcaggctc agacacaggg accagcagtg tctgtggcct
      961 ttttgtgctc ctctccatgc tgggttttaa cttgctcttt gtccttctat cctatcttct
     1021 tatccttaag gctgttctga acgctgtgac ttggagagtg tcccagagcc ctcaacacct
     1081 gcatgtccca cgtccatgct gtcctgcact tccttatccc caagatctgc ctctccgtga
     1141 tgcactgaat tggcaaacat gtgtcacccc agaccaacaa tgtcacagca aactccccct
     1201 tgataggaca agggggaatg gctttacact gagacagggg aggtttgggt tggatatgag
     1261 gaggcagttt ttcccccaga gggtggtgac gcactgaaca ggttgcccaa ggaggctgtg
     1321 gatgccccat ccctgcaggc attcaaggcc aggctggatg tggctctggg cagcctgggc
     1381 tgctggttga tgaccctgca catagcaggg ggttggatct ggatgagcac tgtgctcctt
     1441 tgcaacccag gccgttctat gattctgtca ttctaaatct ctctttcagc ctaaagcttt
     1501 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
     1561 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga
     1621 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg
     1681 gggagggacg taattacatc cctgggggct ttgggggggg gctgtccctc tagcgagctc
     1741 ggtacccaga gccccatcct cactgactcc gtcctggagt tggatgagag ataatggcct
     1801 tacgttgtgc caggggaggg tcgggctgga tttagcaaga tttaccttct ccaaagagcg
     1861 gtgctgcagt ggcacagctg cccacggagg tgggggggtc accgtccctg gaggtgatga
     1921 agaactgtgg ggatgtggca ctgagggaca tggccagtgg gcacggtggg tgggttgggg
     1981 ttggtcttgg ggatcttgga gggcttttcc agccttcatg atttgacgat tgtatgaaca
     2041 tctacatggc aattctccag ctgcctgtcc cagtcctact gacccagctg tatctctcca
     2101 ggcaagctct tccacccctt ctgcttgcat ccagacacca tcaaacatgc aggctcagac
     2161 acagggacca gcagtgtctg tggccttttt gtgctcctct ccatgctggg ttttaacttg
     2221 ctctttgtcc ttctatccta tcttcttatc cttaaggctg ttctgaacgc tgtgacttgg
     2281 agagtgtccc agagccctca acacctgcat gtcccacgtc catgctgtcc tgcacttcct
     2341 tatccccaag atctgcctct ccgtgatgca ctgaattggc aaacatgtgt caccccagac
     2401 caacaatgtc acagcaaact cccccttgat aggacaaggg ggaatggctt tacactgaga
     2461 caggggaggt ttgggttgga tatgaggagg cagtttttcc cccagagggt ggtgacgcac
     2521 tgaacaggtt gcccaaggag gctgtggatg ccccatccct gcaggcattc aaggccaggc
     2581 tggatgtggc tctgggcagc ctgggctgct ggttgatgac cctgcacata gcagggggtt
     2641 ggatctggat gagcactgtg ctcctttgca acccaggccg ttctatgatt ctgtcattct
     2701 aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa
     2761 agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct
     2821 gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg
     2881 ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg
     2941 gggggggctg tccctctagg ggtacccggg gatccggtct cgcgtaccct ttcgagttca
     3001 agaattcctc gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta
     3061 tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga
     3121 gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag
     3181 aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta
     3241 tcagtgatag agaacgtata agctttaggc gtgtacggtg ggcgcctata aaagcagagc
     3301 tcgtttagtg aaccgtcaga tcgcctggag caattccaca acacttttgt cttataccaa
     3361 ctttccgtac cacttcctac cctcgtaaag tcgacggatc cgtttaaagg tgcatgaggg
     3421 cccttgtagt tatcaggttg agtagggtta cggatgcaac caccagcccg gagcgccaac
     3481 tggaatcatg tcagcagctt tgtgcgcagc gcggctggga cgtggtggga gtggcggagg
     3541 acttggacgt tagcggggcc gttgacccat ttgaccgaaa gcggagacct aacctggctc
     3601 gatggctcgc ctttgaggag cagcccttcg atgtgatcgt cgcatacagg gtcgacagac
     3661 tgaccagatc cattcgccat ctgcagcagc tcgttcactg ggcggaggac cacaaaaagc
     3721 tcgtggtgag tgcaacagaa gcccactttg acaccacaac acccttcgca gccgtcgtga
     3781 tcgctctgat gggtaccgtt gcccagatgg aattggaggc aatcaaggag cggaacagat
     3841 ccgccgctca tttcaatatc cgcgcgggca agtacagggg tagtctccca ccctgggggt
     3901 atttgcctac ccgggtggac ggcgaatgga ggcttgttcc cgatcccgtg cagcgagagc
     3961 gaatactgga agtttatcat cgagtcgtgg ataaccatga accactccac ctggtggccc
     4021 acgaccttaa ccgacgcggc gtgctgagcc ctaaggacta ttttgctcaa cttcagggaa
     4081 gagagccaca gggtagggaa tggtcagcca cagctctcaa gcggtctatg atttccgaag
     4141 caatgctcgg gtacgcaaca ctcaatggca agacagttcg agacgacgac ggggcccccc
     4201 tggttcgggc cgaacccata cttacccgcg aacaactgga ggcacttcgc gcggaacttg
     4261 tgaaaacaag ccgagccaaa cccgcagtga gcaccccatc actgctgctg agggtgctct
     4321 tctgtgccgt gtgcggcgaa ccagcataca agttcgctgg cgggggtcga aaacaccccc
     4381 gctaccggtg tcgctcaatg ggttttccaa agcactgtgg caacggaaca gttgcaatgg
     4441 ccgaatggga cgctttttgt gaagaacaag tgctggatct tctgggcgac gctgagaggc
     4501 tggaaaaagt atgggtggcc gggagcgaca gcgccgttga gctcgccgag gtgaacgccg
     4561 aattggtgga cctgacgagt ctcatcggat ctccagcata ccgagctgga tccccccagc
     4621 gagaggctct ggacgctcgg atagccgccc tggcagcaag gcaggaggag cttgaggggt
     4681 tggaagcacg gccttcagga tgggaatggc gggaaacagg acagagattt ggagactggt
     4741 ggagggaaca ggataccgct gctaagaaca cttggctcag gtccatgaat gttcgactca
     4801 ccttcgacgt gaggggtggg ttgacccgca ccattgattt cggggatctg caggagtatg
     4861 aacagcatct ccggcttggc tccgtggtag aaagacttca tacaggcatg tcaggtggat
     4921 caggtggagg cagtggcatg atcagtctga ttgcggcgtt agcggtagat tacgttatcg
     4981 gcatggaaaa cgccatgccg tggaacctgc ctgccgatct cgcctggttt aaacgcaaca
     5041 ccttaaataa acccgtgatt atgggccgcc atacctggga atcaatcggt cgtccgttgc
     5101 caggacgcaa aaatattatc ctcagcagtc aaccgagtac ggacgatcgc gtaacgtggg
     5161 tgaagtcggt ggatgaagcc atcgcggcgt gtggtgacgt accagaaatc atggtgattg
     5221 gcggcggtcg cgttattgaa cagttcttgc caaaagcgca aaaactgtat ctgacgcata
     5281 tcgacgcaga agtggaaggc gacacccatt tcccggatta cgagccggat gactgggaat
     5341 cggtattcag cgaattccac gatgctgatg cgcagaactc tcacagctat tgctttgaga
     5401 ttctggagcg gcgagcatgc taaacctcga gaccacctgc taaggtctgt gccttctagt
     5461 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5521 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5581 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agagaatagc
     5641 aggcatgctg gggatgcggt gggctctatg gtactctagt ctagagcccc atcctcactg
     5701 actccgtcct ggagttggat gagagataat ggccttacgt tgtgccaggg gagggtcggg
     5761 ctggatttag caagatttac cttctccaaa gagcggtgct gcagtggcac agctgcccac
     5821 ggaggtgggg gggtcaccgt ccctggaggt gatgaagaac tgtggggatg tggcactgag
     5881 ggacatggcc agtgggcacg gtgggtgggt tggggttggt cttggggatc ttggagggct
     5941 tttccagcct tcatgatttg acgattgtat gaacatctac atggcaattc tccagctgcc
     6001 tgtcccagtc ctactgaccc agctgtatct ctccaggcaa gctcttccac cccttctgct
     6061 tgcatccaga caccatcaaa catgcaggct cagacacagg gaccagcagt gtctgtggcc
     6121 tttttgtgct cctctccatg ctgggtttta acttgctctt tgtccttcta tcctatcttc
     6181 ttatccttaa ggctgttctg aacgctgtga cttggagagt gtcccagagc cctcaacacc
     6241 tgcatgtccc acgtccatgc tgtcctgcac ttccttatcc ccaagatctg cctctccgtg
     6301 atgcactgaa ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc aaactccccc
     6361 ttgataggac aagggggaat ggctttacac tgagacaggg gaggtttggg ttggatatga
     6421 ggaggcagtt tttcccccag agggtggtga cgcactgaac aggttgccca aggaggctgt
     6481 ggatgcccca tccctgcagg cattcaaggc caggctggat gtggctctgg gcagcctggg
     6541 ctgctggttg atgaccctgc acatagcagg gggttggatc tggatgagca ctgtgctcct
     6601 ttgcaaccca ggccgttcta tgattctgtc attctaaatc tctctttcag cctaaagctt
     6661 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
     6721 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
     6781 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
     6841 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct ctagagcccc
     6901 atcctcactg actccgtcct ggagttggat gagagataat ggccttacgt tgtgccaggg
     6961 gagggtcggg ctggatttag caagatttac cttctccaaa gagcggtgct gcagtggcac
     7021 agctgcccac ggaggtgggg gggtcaccgt ccctggaggt gatgaagaac tgtggggatg
     7081 tggcactgag ggacatggcc agtgggcacg gtgggtgggt tggggttggt cttggggatc
     7141 ttggagggct tttccagcct tcatgatttg acgattgtat gaacatctac atggcaattc
     7201 tccagctgcc tgtcccagtc ctactgaccc agctgtatct ctccaggcaa gctcttccac
     7261 cccttctgct tgcatccaga caccatcaaa catgcaggct cagacacagg gaccagcagt
     7321 gtctgtggcc tttttgtgct cctctccatg ctgggtttta acttgctctt tgtccttcta
     7381 tcctatcttc ttatccttaa ggctgttctg aacgctgtga cttggagagt gtcccagagc
     7441 cctcaacacc tgcatgtccc acgtccatgc tgtcctgcac ttccttatcc ccaagatctg
     7501 cctctccgtg atgcactgaa ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc
     7561 aaactccccc ttgataggac aagggggaat ggctttacac tgagacaggg gaggtttggg
     7621 ttggatatga ggaggcagtt tttcccccag agggtggtga cgcactgaac aggttgccca
     7681 aggaggctgt ggatgcccca tccctgcagg cattcaaggc caggctggat gtggctctgg
     7741 gcagcctggg ctgctggttg atgaccctgc acatagcagg gggttggatc tggatgagca
     7801 ctgtgctcct ttgcaaccca ggccgttcta tgattctgtc attctaaatc tctctttcag
     7861 cctaaagctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc
     7921 gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc
     7981 cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg
     8041 ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct
     8101 ctagagtcga cggcgcgggc cgcatcgaat tctaccgggt aggggaggcg cttttcccaa
     8161 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     8221 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     8281 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     8341 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     8401 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     8461 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     8521 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     8581 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     8641 gcaatcgccg ctagccgcca ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     8701 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     8761 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     8821 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     8881 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
     8941 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     9001 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     9061 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     9121 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgtaag
     9181 tgctagcccc taggcctgaa tcgaagttcc tattcttcaa aaggtatagg aacttcgagc
     9241 tcgtcgactg cagaggcctg catgcaagct tggcgtaatc atggtcatag ctgtttcctg
     9301 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9361 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9421 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9481 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9541 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9601 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9661 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9901 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10021 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10081 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10141 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10201 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10261 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10321 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10381 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10441 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10501 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10561 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10621 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10681 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10741 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10801 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10861 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10921 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10981 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11041 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11101 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11161 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11221 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11281 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11341 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11401 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
    11461 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
