LOCUS       sequence-314494-        6863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synaptic Tag for Mapping Activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Perez-Alvarez A, Fearey BC, O'Toole RJ, Yang W, Arganda-Carreras I, 
            Lamothe-Molina PJ, Moeyaert B, Mohr MA, Panzera LC, Schulze C, 
            Schreiter ER, Wiegert JS, Gee CE, Hoppa MB, Oertner TG
  TITLE     Freeze-frame imaging of synaptic activity using SynTagMA.
  JOURNAL   Nat Commun. 2020 May 18;11(1):2464. doi: 10.1038/s41467-020-16315-4.
  PUBMED    32424147
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-05-18"; volume: "11(1):2464. doi"; pages: " 
            10.1038/s41467-020-16315-4"
COMMENT     Sequence Label: SynTagMA_pre
FEATURES             Location/Qualifiers
     source          1..6863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   47..176
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        221..668
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             1732..1749
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1756..2196
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVVMMTAK"
     CDS             2917..2973
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     misc_feature    3036..3624
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3089..3109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3507..3518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3656..4132
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3855..3874)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4172..4312
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4172..4301
                     /label=AAV2 ITR
     rep_origin      4387..4842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4474..4493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4684..4705
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4859..4878)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4978..5000
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5038..5056)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5124..5228
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5229..5297,5298..6089)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5447..5466)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6260..6848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6749..6768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtcctg caggcagctg
       61 cgcgctcgct cgctcactga ggccgcccgg gcgtcgggcg acctttggtc gcccggcctc
      121 agtgagcgag cgagcgcgca gagagggagt ggccaactcc atcactaggg gttcctgcgg
      181 ccgcacgcgt gtgtctagac tgcagagggc cctgcgtatg agtgcaagtg ggttttagga
      241 ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc
      301 acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga
      361 tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc
      421 ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc
      481 ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg
      541 gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg
      601 ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga
      661 gagcgcagtc gagaaggtac cggatccccg ggtaccggta tggacgtggt gaatcagctg
      721 gtggctgggg gtcagttccg ggtggtcaag gagccccttg gcttcgtgaa ggtgctgcag
      781 tgggtctttg ccatcttcgc ctttgctacg tgcggcagct acaccggaga gcttcggctg
      841 agcgtggagt gtgccaacaa gacggagagt gccctcaaca tcgaagtcga atttgagtac
      901 ccattcaggc tgcaccaagt gtactttgat gcaccctcct gcgttaaagg gggcactacc
      961 aagatcttcc tagttggtga ctactcctcc tcggctgaat tctttgtcac cgtggctgtg
     1021 tttgccttcc tctactccat gggggccctg gccacctaca tcttcctgca gaacaagtac
     1081 cgagagaaca acaaagggcc aatgatggac ttcctggcca cagcagtgtt cgctttcatg
     1141 tggctagtta gctcatccgc ctgggccaaa ggcctgtccg atgtgaagat ggccactgac
     1201 ccagagaaca ttatcaagga gatgcctatg tgccgccaga caggaaacac atgcaaggaa
     1261 ctgagggacc ctgtgacttc aggactcaac acctcggtgg tgtttggctt cctgaacctg
     1321 gtgctctggg ttggcaacct atggttcgtg ttcaaggaga caggctgggc cgccccattc
     1381 atgcgcgcac ctccaggcgc cccagaaaag caaccagctc ctggcgatgc ctacggcgat
     1441 gcgggctatg ggcagggccc cggaggctat gggccccagg actcctacgg gcctcagggt
     1501 ggttatcaac ccgattacgg gcagccagcc agcggcggtg gcggtggcta cgggcctcag
     1561 ggcgactatg ggcagcaagg ctacggccaa cagggtgcgc ccacctcctt ctccaatcag
     1621 atgaagggcg tggagcccaa gacctactgc tactacagca gcaagcttat gctgcagaac
     1681 gagcttgctc ttaagttggc tggacttgat attaacaaga ctggaggttc tcatcatcac
     1741 caccaccatg gatccgacca actgactgaa gagcagatcg cagaatttaa agaggctttc
     1801 tccctatttg acaaggacgg ggatgggaca ataacaacca aggagctggg gacggtgatg
     1861 cggtctctgg ggcagaaccc cacagaagca gagctgcagg acatgatcaa tgaagtagat
     1921 gccgacggtg acggcacaat cgacttccct gagttcctga caatgatggc aagaaaaatg
     1981 aaagacacag acagtgaaga agaaattaga gaagcgttcc gtgtgtttga taaggatggc
     2041 aatggctaca tcagtgcagc agagcttcgc cacgtgatga caaaccttgg agagaagtta
     2101 acagatgaag aggttgatga aatgatcagg gaagcagaca tcgatgggga tggtcaggta
     2161 aactacgaag agtttgtagt gatgatgaca gcgaagctcg agtgcgagaa aatctatgtg
     2221 cgtgatggag tgctgacggg tgatattcat atgatcttgt tgcttgaagg aaatgcccat
     2281 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
     2341 gtgcactatg tggaccacac cattgagatt ttaagccatg acaaagatta caacaaggtt
     2401 aagatctatg agtatgctgt tgctcattct ggattgcctg acaatgccag acgaggcggt
     2461 accggcggat ccatggtgag tgcgattaag ccagacatga agatcaaact gcgtatggaa
     2521 ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttatgag
     2581 ggaaaacaga ccatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat
     2641 atcctgacca ctgcattcca ttacggcaac agggtattcg tgaaatatcc agacaacata
     2701 caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag catgactttc
     2761 gaagacgggg gcatttgcta tgccagaagc gacataacaa tggaagggga cactttctat
     2821 aataaagttc gattttatgg taccaacttt cccgccaatg gtccagttat gcagaagaag
     2881 acgctgaaat ggatgccgag ctggacgcgt tcatcacgtc gtaagtggaa taagacaggt
     2941 cacgcagtca gagctatagg tcggctgagc tcataatgta caagttctgc tacgagaacg
     3001 aggtgtaatg agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     3061 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3121 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3181 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3241 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3301 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3361 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3421 tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc
     3481 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3541 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3601 tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg
     3661 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
     3721 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
     3781 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg
     3841 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac
     3901 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg
     3961 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt
     4021 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc
     4081 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt
     4141 gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     4201 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4261 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     4321 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     4381 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4441 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
     4501 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4561 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     4621 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4681 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
     4741 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
     4801 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     4861 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     4921 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     4981 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5041 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5101 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5161 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5221 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5281 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5341 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5401 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5461 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5521 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5581 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5641 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5701 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5761 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5821 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5881 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5941 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6001 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6061 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6121 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6181 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6241 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6301 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6361 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6421 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6481 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6541 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6601 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6661 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6721 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6781 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6841 tatggaaaaa cgccagcaac gcg
//
