LOCUS       sequence-314500-       13700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Ago-Dcr fusion protein in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13700)
  TITLE     Yeast viruses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pESC-NAT-Ago-Dcr
FEATURES             Location/Qualifiers
     source          1..13700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..665
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     primer_bind     complement(14..37)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    332..449
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             4642..4665
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      4813..4978
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4868..4892)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     rep_origin      5057..5512
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5144..5163)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5354..5375
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     gene            complement(6253..7372)
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     terminator      complement(6253..6450)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(6456..7028)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(7029..7372)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(8020..8039)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8139..8161
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8199..8217)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      8258..9600
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    8590..8637
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        9627..9731
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9732..9800,9801..10592)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9950..9969)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10763..11351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11252..11271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11505..11522
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(11594..11783)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(13671..13690)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13672..13690)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttttttctc cttgacgtta aagtatagag gtatattaac aattttttgt tgatactttt
       61 attacatttg aataagaagt aatacaaacc gaaaatgttg aaagtattag ttaaagtggt
      121 tatgcagttt ttgcatttat atatctgtta atagatcaaa aatcatcgct tcgctgatta
      181 attaccccag aaataaggct aaaaaactaa tcgcattatc atcctatggt tgttaatttg
      241 attcgttcat ttgaaggttt gtggggccag gttactgcca atttttcctc ttcataacca
      301 taaaagctag tattgtagaa tctttattgt tcggagcagt gcggcgcgag gcacatctgc
      361 gtttcaggaa cgcgaccggt gaagacgagg acgcacggag gagagtcttc cttcggaggg
      421 ctgtcacccg ctcggcggct tctaatccgt acttcaatat agcaatgagc agttaagcgt
      481 attactgaaa gttccaaaga gaaggttttt ttaggctaag ataatggggc tctttacatt
      541 tccacaacat ataagtaaga ttagatatgg atatgtatat ggatatgtat atggtggtaa
      601 tgccatgtaa tatgattatt aaacttcttt gcgtccatcc aaaaaaaaag taagaatttt
      661 tgaaaattcg aattcaaccc tcactaaagg gcggccgcaa cgatgtcatc caattcggag
      721 gagaacagtc aagttcctcc ccttgatgcc accgctgctg caactaaacc aaaaaaggcc
      781 aagaaaccaa aggttaagaa gccaaaggat tccgctgagg catcttcatc accagctgct
      841 gagggcactg ctgaagccaa gccaaaaaag gctaagaaat caaagactaa gaagtcaaag
      901 gagtctgccg aggtatcgcc agctccagct gatgaaacta cttccgctgg tgtagatgct
      961 aaacctaaga aggctaaaaa gtcaaaggtc aagaagccaa aggattctac tgaatcatct
     1021 cctgctccaa gtaatgaacc tccagccgcc gaagttgccg ccgaagatgc aaagtctaaa
     1081 aaggttaaga aaccaaaggc caagaaacca aaggaatcta ctgaatcatc tcctgctcca
     1141 ggtcaggaag ccgctgctac tgaaggtgcc acagaagata aacctaagaa ggttaagaaa
     1201 tcaaaggcta agaaggcaaa ggaatctgtt gaatcatctc ctgccgccac tgaatctgtc
     1261 tctgagaaga ctgcaaagaa atctaagaag ccaaaggcta agaaatctac ttctcctgaa
     1321 acaactgaag aaataactga agaatctacc gaatcaaagg aaaagaagac aaagacaaag
     1381 aaaccaaagg aaaagaagtc atctccatct actgccactt cgactgctgc ttcaaaacca
     1441 gttacttcca ttgcgggagt caccattccg ggcaagactt ttgatttgac agatgttcct
     1501 ccatctaaac ctgctccaaa gatggtcccg gaagcctata aattacaaac tagagtggat
     1561 tacggtacta agggtaccaa agtggacgtc ttgactaatc atatactact atctgttggt
     1621 gatgatgtcc ctcaagatga aagagcctct caattggatc catggtggaa atctgcattt
     1681 gtatatacct ataacattac ttttgccgtt ccacagagta attcaccacg taaaggtcca
     1741 gcgcctgctt tatccaaacc aaagaaatac gaattggtag aatctttgtt tcacgaggat
     1801 gaaaccttgt tcaaatataa agatcgtatc tccttcaatg gtgaagatac tctatactct
     1861 catgtcccat tggaggaatt cactttattt gatggttgtt gggatgtcag taacaagcaa
     1921 aagaaaagaa gacaagaagt tgttggtctc aacagtagag ccaaggaaat taatgacttg
     1981 gctgctcaag ttactttaaa atttgctgat aaagtcccat tgggtgatat ttataaggct
     2041 accacttcaa aggatccaga agaacaagaa aataagatgg ctaacgctga taaggttgct
     2101 ttattgtctt tgatgggtgt caaattctta aacacaaagg aacaaatctt ccaacttaac
     2161 ggtaataaat tctttatttt taatggacac gcaattgcta ctccattcca aattggtggg
     2221 ttcttaatgc atgggttcac agtttcccta cgatatgctt acgggtccgt tttgttaaat
     2281 actgttaacg tctgcctccc atttgttaag tggactaaat atttaccagg tgatgctaaa
     2341 ttcaaggaaa atgaaaagac tcaatacagt ttgttagatt ggattattga atgtatgcac
     2401 caggccagtg ctcaaagagg tcaaaagcta agaggccctc catctgccaa ggatatcaat
     2461 tttttcattg acaagaatag agacattaaa gatctattga agggtttgaa atgttacaga
     2521 ccatacatta attattctgt taacccagat ggaactccaa aaccaccaaa aaagatgcaa
     2581 gctaagggta tcgttggttt cgtaagagag actccagatt ctatgaaatt cagaacttta
     2641 ccaagtaaca tggaaaagaa tggtgtacca aaaccaggtg aaaaggaaat catggtcaca
     2701 acaaccgcct actttgccaa gaagtatgac atcaaattga aatacccaga tgttaaaatg
     2761 gtgagtctag gtggttcgaa tgttgtccca gctgaatgtt taaccattgt tccaggtcag
     2821 aaattaaagg gtttggttta tgacgaaaaa gccgttattg atttcactgc tttaagacca
     2881 agtgaaaaat tcagagccat caccaacttg gcattacctg ccattaagag agctttatca
     2941 actgaagaag aaaatgctaa ggctccacat gattctggtt acactttcat gaaggtccca
     3001 tctcgtgtca ttgacgctcc tgtggttcaa ttcaagaaca ctacagtcac atatgtggat
     3061 aaaccatttg gtactaagaa tggtaagaat aatcatgaag aaactaaggg taattggaat
     3121 ttaaaggatc ataaattcat tactgttcca aaggaaccaa tgcacttgag agctatcttt
     3181 attaatgatt ctgataagtc tccaccagtt tctgtcatgg atgaattgaa ggcctctttg
     3241 agcaaattcg ctgaagatgt cgctgatgtc ggtgttaact ttgacgtatc catggcccca
     3301 attttaatta acaattttaa tgccccaata aagaaggtta ctggtggctt tggtggtaga
     3361 ggtggacgtg gtggtagagg cggccgtggt ggccgtggtg gtagaggagg tcgtggtggt
     3421 ttcggcggtg gccgtggtga aactacttat gaattaactc caggtgaaga aaaactacgt
     3481 cacttgttag ccaacgtccc agaaaagact tatgtcttat tcgtcttggg tcgtggggat
     3541 gactctgcta tttacaacag attgaaatat ttagctgatt tgacgtacgg tgtcattaac
     3601 aattgtgtta tttggaacaa gttcagaaag tgttccactc aatacaatgt taatgtggta
     3661 atgaagatga acttgaagtt agaaggtgct aaccactcct tatgtgcaga agatataaac
     3721 ttactgaagg atgaaaaatc aggattacca ttcatgattt taggtgctga tgttacacat
     3781 tatccagaaa aggatcagaa ttctatctct gcattggtgg gttcttttga tgacaaattt
     3841 gctcaattcc caggttcata tatgttacaa agtggtccag gtgaagaaat aattgctggt
     3901 atcggtaaca tggttctaca gagattgaaa ctgtatcaaa agcacaacaa tggcaaacta
     3961 cctccaaaga ttctattcta cagagatggt gtttctgaat cccaattctc acaaattgtt
     4021 caaattgaag ttaagggttt gaaacaagca ttgaagaaat tcggtagtga attaaacaag
     4081 ggtgtcaact ataacccatc tgtcaccaca atttgtgttg tcaagagaaa tcaaattaga
     4141 tttatgccac ttgagcaaaa tgctataaat gaaaagggtg aagttgctgc cgttcaatca
     4201 ttcgagaacg ttatgccagg tactgttgtt gatcgtggta tcacctctag tgctcacttc
     4261 gatttcttct tgcaatctca acaaccattg aagggtactg gtgttccatg tcattactgg
     4321 tgtatctacg acgaaaatca attcaattct gattacttgc aacaagttac tcacgctttg
     4381 tgttacttat tcggtagatc gagtacaagt attaaggtcg catctcctgt ttattacgct
     4441 gatttattgt gtgaacgtgg tgctgcattc ttcaaggcta actttgagct tgctcaatac
     4501 gaattttcta aggagaggaa gaacagagat gacgttatac cgaccggtaa attactacaa
     4561 cctgttcata agaatgtcac tgacatcatg tactacatat gaacaaagag tttatgtgct
     4621 gcggccgcac tagtatcgat ggattacaag gatgacgacg ataagatctg agctcttaat
     4681 taacaattct tcgccagagg tttggtcaag tctccaatca aggttgtcgg cttgtctacc
     4741 ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata cgttgttgac
     4801 acttctaaat aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa
     4861 aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc
     4921 ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctcca
     4981 attcagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5041 cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5101 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5161 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5221 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5281 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5341 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5401 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5461 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
     5521 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatcgacc ggtcgaggag
     5581 aacttctagt atatctacat acctaatatt attgccttat taaaaatgga atcccaacaa
     5641 ttacatcaaa atccacattc tcttcaaaat caattgtcct gtacttcctt gttcatgtgt
     5701 gttcaaaaac gttatattta taggataatt atactctatt tctcaacaag taattggttg
     5761 tttggccgag cggtctaagg cgcctgattc aagaaatatc ttgaccgcag ttaactgtgg
     5821 gaatactcag gtatcgtaag atgcaagagt tcgaatctct tagcaaccat tatttttttc
     5881 ctcaacataa cgagaacaca caggggcgct atcgcacaga atcaaattcg atgactggaa
     5941 attttttgtt aatttcagag gtcgcctgac gcatatacct ttttcaactg aaaaattggg
     6001 agaaaaagga aaggtgagag cgccggaacc ggcttttcat atagaataga gaagcgttca
     6061 tgactaaatg cttgcatcac aatacttgaa gttgacaata ttatttaagg acctattgtt
     6121 ttttccaata ggtggttagc aatcgtctta ctttctaact tttcttacct aggccactag
     6181 tggatctgat atcatcgatg aattcgagct cgttttcgac actggatggc ggcgttagta
     6241 tcgaatcgac agcagtatag cgaccagcat tcacatacga ttgacgcatg atattacttt
     6301 ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg cgaaaaaaaa tataaatcac
     6361 gctaacattt gattaaaata gaacaactac aatataaaaa aactatacaa atgacaagtt
     6421 cttgaaaaca agaatctttt tattgtcagt actgattagg ggcagggcat gctcatgtag
     6481 agcgcctgct cgccgtccga ggcggtgccg tcgtacaggg cggtgtccag gccgcagagg
     6541 gtgaacccca tccgccggta cgcgtggatc gccggtgcgt tgacgttggt gacctccagc
     6601 cagaggtgcc cggcgccccg ctcgcgggcg aactccgtcg cgagccccat caacgcgcgc
     6661 ccgaccccgt gcccccggtg ctccggggcg acctcgatgt cctcgacggt cagccggcgg
     6721 ttccagccgg agtacgagac gaccacgaag cccgccaggt cgccgtcgtc cccgtacgcg
     6781 acgaacgtcc gggagtccgg gtcgccgtcc tccccgtcgt ccgattcgtc gtccgattcg
     6841 tcgtcgggga acaccttggt caggggcggg tccaccggca cctcccgcag ggtgaagccg
     6901 tccccggtgg cggtgacgcg gaagacggtg tcggtggtga aggacccatc cagtgcctcg
     6961 atggcctcgg cgtcccccgg gacactggtg cggtaccggt aagccgtgtc gtcaagagtg
     7021 gtacccatgg ttgtttatgt tcggatgtga tgtgagaact gtatcctagc aagattttaa
     7081 aaggaagtat atgaaagaag aacctcagtg gcaaatccta accttttata tttctctaca
     7141 ggggcgcggc gtggggacaa ttcaacgcgt ctgtgagggg agcgtttccc tgctcgcagg
     7201 tctgcagcga ggagccgtaa tttttgcttc gcgccgtgcg gccatcaaaa tgtatggatg
     7261 caaatgatta tacatgggga tgtatgggct aaatgtacgg gcgacagtca catcatgccc
     7321 ctgagctgcg cacgtcaaga ctgtcaagga gggtattctg ggcctccatg tcgctggccg
     7381 ggtgacccgg cggggacgag gcaagctaaa cagatctggc gcgccttaat taacccgggg
     7441 atccgtcgac ctgcagcgta cgaagcttca gctgcaccga agtcggtgat gctgtcgccg
     7501 aagaagttaa gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa
     7561 actttataaa tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat
     7621 agggtagacg aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg
     7681 atgtaaagga atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga
     7741 attgcacttt aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa
     7801 cagaaaatca tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa
     7861 aaaaatacaa caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat
     7921 accttcttga accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga
     7981 aacggcctta acgacgtagt cgatatggtg cactctcagt acaatctgct ctgatgccgc
     8041 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     8101 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     8161 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     8221 ataggttaat gtcatgataa taatggtttc ttagtatgat ccaatatcaa aggaaatgat
     8281 agcattgaag gatgagacta atccaattga ggagtggcag catatagaac agctaaaggg
     8341 tagtgctgaa ggaagcatac gataccccgc atggaatggg ataatatcac aggaggtact
     8401 agactacctt tcatcctaca taaatagacg catataagta cgcatttaag cataaacacg
     8461 cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata taggtgcgac
     8521 gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg
     8581 aaacgctttg aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcaga
     8641 gcgcttttga aaaccaaaag cgctctgaag acgcactttc aaaaaaccaa aaacgcaccg
     8701 gactgtaacg agctactaaa atattgcgaa taccgcttcc acaaacattg ctcaaaagta
     8761 tctctttgct atatatctct gtgctatatc cctatataac ctacccatcc acctttcgct
     8821 ccttgaactt gcatctaaac tcgacctcta cattttttat gtttatctct agtattactc
     8881 tttagacaaa aaaattgtag taagaactat tcatagagtg aatcgaaaac aatacgaaaa
     8941 tgtaaacatt tcctatacgt agtatataga gacaaaatag aagaaaccgt tcataatttt
     9001 ctgaccaatg aagaatcatc aacgctatca ctttctgttc acaaagtatg cgcaatccac
     9061 atcggtatag aatataatcg gggatgcctt tatcttgaaa aaatgcaccc gcagcttcgc
     9121 tagtaatcag taaacgcggg aagtggagtc aggctttttt tatggaagag aaaatagaca
     9181 ccaaagtagc cttcttctaa ccttaacgga cctacagtgc aaaaagttat caagagactg
     9241 cattatagag cgcacaaagg agaaaaaaag taatctaaga tgctttgtta gaaaaatagc
     9301 gctctcggga tgcatttttg tagaacaaaa aagaagtata gattctttgt tggtaaaata
     9361 gcgctctcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa
     9421 ttagcgctct cgcgttgcat ttttgtttta caaaaatgaa gcacagattc ttcgttggta
     9481 aaatagcgct ttcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg
     9541 aaaaattagc gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt
     9601 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9661 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9721 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9781 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9841 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9901 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9961 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    10021 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10081 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10141 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10201 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10261 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10321 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10381 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10441 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10501 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10561 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10621 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10681 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10741 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10801 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10861 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10921 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10981 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    11041 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11101 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    11161 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11221 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11281 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11341 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11401 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11461 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11521 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11581 aatgcagctg gatcttcgag cgtcccaaaa ccttctcaag caaggttttc agtataatgt
    11641 tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata aataacgttc
    11701 ttaatactaa cataactata aaaaaataaa tagggaccta gacttcaggt tgtctaactc
    11761 cttccttttc ggttagagcg gatcttagct agccgcggta ccaagcttac tcgagttcac
    11821 agattgttgc aatgcctcaa gtattccttc agtgcttttc cattcaaagc atcttctgca
    11881 gatttatgtt gggatatctt tttacttcta ccatggcctt taccaatttc tacaccaaca
    11941 ccctttataa agcataccgt ataaaaatct gtcattccta gttgctcggt attatattct
    12001 ggatacaatt tatatttacc taacaatgca taaagtctcc ctgaggcttc tttatcatac
    12061 acagaattta aaagtgcaac ttcaggcgtt ttatttaggt ttgtgcttac cggttgtgtg
    12121 accattttgg gttgtggtgg attattcaca actgatgggg tgacagctac ggctggagct
    12181 ggcgctggga ctgagactga gactggggat gttgctagcg gtgagactgg ggttgttact
    12241 gggataggga taggtgcatt ttttggagct ggtctaccaa gtgcatttga aacagcctca
    12301 gaaaccatca ccgacagtat cttactcata cgttccataa catcattcac aattttatca
    12361 gtgtcaggca cattttgttc ttgatgatcc gcagaagcct gtggaatttg attagttaca
    12421 atttcaggta aaactgctgg tggtgtacct ttcaaaaatg gattcgaatg ggggaatgta
    12481 agaccttctt cttcatcaat ttcttctgtg atagaatgat gactttgtgg tgtttgattg
    12541 ggaatttccg cacttttgag taattgggga acattatctg cttcttcaat catgcctctt
    12601 tctagttcaa tatcatgtag agaatatttt tgaatcaatt tcggttgagc aagtgcttcc
    12661 atagcggctc tctgttcagc ttctctcaca tttgaaccat ctccaatacc taacaaatta
    12721 ttccccaatc ttacttctac cttaaacgga gatttatcat tcaatctttt atatgatatt
    12781 ttggctccaa tattattaaa ttgtaagaac gcacctagtt caccctttgc atttttgttt
    12841 aaaggctctt tgaccatcat aggtcctagt tcaatgaaat gttcctcaga tagttcttca
    12901 agccaaagag caacatcatt aaattctgtt gaaaatctat caataaccaa tgcgccaatg
    12961 taggcttcca cacagtctgc gtaattcttg gttaatctat ctttgatttt cattgaagaa
    13021 cgtggaatat tttcctttaa tctttcttta aagccaagtt tttcacagat cttttccaaa
    13081 ttgttgttgt taacgatagc ttccttcatc tttgataagg caccttcatt agcataagga
    13141 tatttcttat aaataatata cgccactaag gcgccaagcc aactatcacc taaaaattcc
    13201 aaacgttcat tactcattac ggtcttttgc aattcagtca atttgacatt catattcggc
    13261 aaagatctat gaatgaaagc caaattttcc aaattttgat cagaacatac gggataatgg
    13321 acataaggat caactggtga tgaattattt tcaaatttat aatgatcaaa gatattcttg
    13381 atgtctaggg tgggtctaac ttggttcaag caacttgcta cggacatagc aggactgttt
    13441 gcaatagatt tttctaggtc gttcccatgt ttcgccatag tattcaattg atcggatgat
    13501 aacccgtttt cgtaaattac ttttatggat tcccccaatt gcgcacatgc attttgaacc
    13561 ttatagaaat ttcttaaatt tttggcatcg agttcattgt atggagcatc tgatgtttta
    13621 cttagatcgg cgcttttttc tctattcatt tgtataattg cgtgtagggg ccctatagtg
    13681 agtcgtatta cggatccggg
//
