LOCUS       sequence-314507-        7274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blue-light dependent heterodimerization system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Benedetti L, Marvin JS, Falahati H, Guillen-Samander A, Looger LL, 
            De Camilli P
  TITLE     Optimized Vivid-derived Magnets photodimerizers for subcellular 
            optogenetics in mammalian cells.
  JOURNAL   Elife. 2020 Nov 11;9. pii: 63230. doi: 10.7554/eLife.63230.
  PUBMED    33174843
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-11-11"; volume: "9. pii: 63230. doi"; pages: " 
            10.7554/eLife.63230"
COMMENT     Sequence Label: ER-EGFP-eMagA
FEATURES             Location/Qualifiers
     source          1..7274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        3..382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..660
                     /label=chicken beta-actin promoter
     intron          661..1669
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1593..1615
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1771..1780
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1921..1923,1924..1926,1927..2637)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     intron          3198..3330
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3313..3332
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    3339..3927
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3392..3412)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3810..3821)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3954..3971)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3960..4071
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4298..4317)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4363..4418
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4417..4436)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4777..4793)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4790..4812)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4801..4817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4825..4831,4832..4849,4850..4855))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4870..4891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4950..5145
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4996..5131
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5151..5285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5188..5207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5242..5261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5353..5370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5524..6112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5604..5623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6283..7074,7075..7143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6906..6925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7144..7248)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
       61 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      181 gactttccat tgacgtcaat gggtggacta tttacggtaa actgcccact tggcagtaca
      241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      361 attagtcatc gctattacca tgggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg gcgagggcgg
      541 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc
      841 cttaaagggc tccgggaggg ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt
      901 gtgtgtgtgc gtggggagcg ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg
     1021 gtgccccgcg gtgcgggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     1081 gggggggtga gcagggggtg tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc
     1141 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg
     1201 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     1261 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg
     1321 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     1381 acttcctttg tcccaaatct ggcggagccg aaatctggga ggcgccgccg caccccctct
     1441 agcgggcgcg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1501 gtgcgtcgcc gcgccgccgt ccccttctcc atctccagcc tcggggctgc cgcaggggga
     1561 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     1621 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     1681 cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa ttcggtaccg cgggcccggg
     1741 atccaccgga agccacctgc atgcggccgc gccaccatgg atcctgtggt ggtgctgggg
     1801 ctctgtctct cctgtttgct tctcctttca ctctggaaac agagctatgg gggagggttg
     1861 gatccaccgg tcggcggctc cggaggcagc ggcggttctg gaggttccgg tggcggctcc
     1921 atggtgtcga agggagagga gcttttcacc ggcgtggtgc cgattcttgt ggaactggac
     1981 ggcgacgtga acggacataa gttcagcgtg tcaggagaag gagaagggga cgcgacttac
     2041 ggaaagctga ccctgaagtt catctgtacc accgggaagc tcccggtgcc ctggccgacc
     2101 ctcgtgacta ctctgaccta cggagtgcag tgcttctccc gctacccgga ccatatgaag
     2161 cagcacgatt tcttcaagag cgctatgccc gagggttacg tgcaagaacg gaccatcttc
     2221 ttcaaggacg acggaaacta caagaccaga gccgaagtga aattcgaagg agacaccctc
     2281 gtcaatcgca tcgagctgaa gggaatcgac tttaaggaag atgggaatat cctgggtcac
     2341 aagctggagt acaactacaa ctcccacaat gtgtacatca tggcggataa gcaaaagaac
     2401 ggcattaagg tcaacttcaa gatccggcat aacatagaag atggctccgt gcaactggcg
     2461 gaccactacc agcagaacac ccccattggg gacggtccgg tgctgctccc tgataaccac
     2521 tacctgtcaa cccaaagcgc actgtccaag gaccccaacg agaagaggga ccacatggtg
     2581 ctgctggagt tcgtcactgc cgctggcatc actctcggaa tggacgagct gtacaagggg
     2641 ggcggctccg gaggcagcgg cggttctgga ggttccggtg gcggctccac gcgtatggga
     2701 cacactcttt acgcccctgg aggatacgac attatgggat atttggatca gattgcgaac
     2761 cgcccaaacc ctcaggtcga actggggcct gtggacctgt catgtgccct gatcctgtgc
     2821 gatctgaagc aaaaggacac tccgatcgtc tacgcctcgg aagccttctt ggagatgacc
     2881 ggatacaaca gacatgaggt gctcggcagg aactgcagat tcctgcagtc ccccgacggg
     2941 atggtgaaac caaagtcgac tcgcaaatat gtggactcga acacgatcta caccatcaag
     3001 aaggccatcg accggaacgc cgaggtccag gtggaggtgg tcaactttaa gaagaacggc
     3061 cagcggttcg tgaactttct gaccatcatt ccggtccggg atgaaaccgg agagtacaga
     3121 tactccatcg gattccagtg cgaaaccgaa tgagttaacc tcgaggtacc gatgaatagc
     3181 taaggtcgag gccgcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga
     3241 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
     3301 ctgacatcca ctttgccttt ctctccacag gtgtcgacaa tcaacctctg gattacaaaa
     3361 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3421 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3481 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3541 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3601 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3661 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3721 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     3781 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3841 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3901 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacgat cagcctcgac
     3961 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4021 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4081 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4141 ggaagacaat agcaggccag cttttgttcc ctttagggtt aattgcgcgc ttggcgctaa
     4201 tcatggtcat agctgtttcc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
     4261 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
     4321 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     4381 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     4441 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat
     4501 gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct
     4561 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt tttttatatt
     4621 ttgtttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     4681 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga
     4741 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4801 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4861 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4921 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     4981 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5041 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5101 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     5161 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5221 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5281 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5341 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5401 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5461 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5521 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5581 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5641 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5701 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5761 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5821 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5881 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5941 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     6001 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6061 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6361 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6421 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6481 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6541 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6601 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6661 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6721 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6781 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6841 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6901 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6961 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7021 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7081 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7141 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7201 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7261 aaaagtgcca cctg
//
