LOCUS       sequence-31451-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBC53
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             581..598
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(651..669)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      665..712
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(836..855)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aattccctct gaatattttg tttactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggttc cgcgtggtag tatgcacggc agagcttacc
      121 tcttgctgca cagagacttc tgtgatctca aggagaacaa ttataagggt atcactgcta
      181 agcctgtaag tgaagatatg atggaatggg aagttgaaat tgaaggtcta cagaattcag
      241 tttggcaggg tttagtcttc caactgacaa tacattttac atcggagtac aactatgctc
      301 ctccagttgt gaaatttata acaattccgt ttcatccaaa tgtagaccca cacactggtc
      361 agccctgtat agactttttg gacaaccctg agaagtggaa tacaaactat acattgagca
      421 gcatcttact tgccctacag gttatgcttt ctaatccagt gctagagaat ccagtgaatt
      481 tggaagcagc cagaatactg gttaaagatg aatctctgta cagaacaatt ctaagacttt
      541 tcaacaggcc atgatgacga agcttgcggc cgcactcgag caccaccacc accaccactg
      601 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      661 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      721 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
      781 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      841 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      901 c
//
