LOCUS       sequence-314516-        6122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blue-light dependent heterodimerization system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Benedetti L, Marvin JS, Falahati H, Guillen-Samander A, Looger LL, 
            De Camilli P
  TITLE     Optimized Vivid-derived Magnets photodimerizers for subcellular 
            optogenetics in mammalian cells.
  JOURNAL   Elife. 2020 Nov 11;9. pii: 63230. doi: 10.7554/eLife.63230.
  PUBMED    33174843
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-11-11"; volume: "9. pii: 63230. doi"; pages: " 
            10.7554/eLife.63230"
COMMENT     Sequence Label: mCherry- eMagBF - 5ptaseOCRL
FEATURES             Location/Qualifiers
     source          1..6122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        145..448
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        449..652
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     603..623
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      690..699
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             696..1403
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(841..859)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1109..1128
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2994..3115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3031..3050)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3085..3104
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3122..3577)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3259..3280)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3471..3490
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3604..3708
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3710..4067
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3710..3901
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3733..3753)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3918..4053
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4102..4896
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4102..4896
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4156..4175)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4766..4785
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5084..5103)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5128..5175
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5504..6092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5993..6012
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
       61 gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt
      121 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      181 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      241 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      301 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      361 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      421 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      481 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      541 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      601 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
      661 gaaccgtcag atccgctagc gctaccggtg ccaccatggt gagcaagggc gaggaggata
      721 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      781 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      841 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      901 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      961 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     1021 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     1081 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     1141 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     1201 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     1261 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     1321 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     1381 gcggcatgga cgagctgtac aagagatctc gaagcgcggc cgcgatggga cataccctct
     1441 acgcgccggg gggttatgac atcatgggtt acctcagaca gatcagaaac cggccgaacc
     1501 cacaagtgga gctgggaccc gtcgacctct cctgcgccct cgtgctgtgt gaccttaagc
     1561 agaaggacac ccctgtggtg tacgcctccg aagcattcct ggagatgacc gggtacaaca
     1621 gacacgaagt gctgggacgg aactgccgct tcctgcaatc cccggatgga atggtgaagc
     1681 ctaagtcaac ccgcaaatac gtggactcca acactatctt caccatgaag aaggccattg
     1741 accgcaatgc tgaggtgcaa gtggaagtgg tgaacttcaa gaagaacgga cagcgcttcg
     1801 tcaacttcct gactatgatt cccgtgcggg acgaaaccgg cgaataccgg tacagcatcg
     1861 ggtttcagtg cgagactgag ggctccggcg gcagcggtgg ctccggaggc agcggaggta
     1921 cccgatcgta tgtcaacatt cagactttca gattttttgt tggaacttgg aatgtgaatg
     1981 gccagtctcc agatagcggg ttagaacctt ggctgaactg tgatcccaat cctcctgata
     2041 tctactgcat tggattccaa gaactggact tgagcacaga agccttcttc tactttgaat
     2101 ctgtgaagga acaagaatgg tccatggctg tagagagagg tttgcattcc aaagccaagt
     2161 ataagaaagt tcaactggtg cgccttgttg ggatgatgct tcttatattt gccagaaagg
     2221 atcagtgtcg atacattcgt gatattgcta cagaaacagt tggaactgga atcatgggga
     2281 aaatgggaaa caaaggtggg gtagctgtga gatttgtatt tcacaacacc accttttgca
     2341 ttgtcaattc ccatctggct gcacacgtgg aggactttga gagaaggaat caagattata
     2401 aggacatttg tgcgagaatg agttttgtgg tcccaaatca gaccctcccg cagttgaaca
     2461 tcatgaaaca tgaggttgtc atttggttgg gagatttgaa ttatagactt tgcatgcctg
     2521 atgccaatga ggtgaaaagt cttattaata agaaagacct tcagagactc ttgaaattcg
     2581 accagctaaa tattcagcgc acacagaaaa aagcttttgt tgacttcaat gaaggggaaa
     2641 tcaagttcat ccccacttat aagtatgact ctaaaacaga ccggtgggat tccagtggga
     2701 aatgccgggt tccagcctgg tgtgaccgaa ttctttggag aggaacaaat gttaatcagc
     2761 ttaattatcg gagtcacatg gaactgaaaa ccagcgacca caagcctgtt agcgccctct
     2821 tccatattgg ggtgaaggtt gtggattgag gtaccgcggg cccgggatcc accggatcta
     2881 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2941 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3001 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3061 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     3121 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     3181 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     3241 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     3301 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     3361 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     3421 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     3481 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3541 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     3601 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3661 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     3721 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3781 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3841 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3901 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3961 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4021 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     4081 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     4141 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     4201 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4261 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     4321 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     4381 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     4441 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4501 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4561 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4621 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     4681 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4741 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     4801 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     4861 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     4921 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     4981 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5041 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     5101 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     5161 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     5221 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     5281 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     5341 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5401 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5461 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5521 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5581 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5641 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5701 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5761 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5821 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5881 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5941 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6001 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6061 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6121 tc
//
