LOCUS       sequence-314529-       13715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes L-A cDNA for curing L-A virus and Parent for pJD1223-NAT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13715)
  TITLE     Yeast viruses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PJD1223
FEATURES             Location/Qualifiers
     source          1..13715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(451..1125)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(1126..1227)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(1247..1265)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1333..1437
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1438..1506,1507..2298)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1656..1675)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2469..3057
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2958..2977
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3211..3228
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3243..3383
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3309..3328)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3469..3491
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3485..3676)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      4939..5452
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5026..5045)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5236..5257
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5650..5669
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     7457..7473
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7458..7474
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12079..12095)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12079..12095)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     rep_origin      join(12463..13715,1..90)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    12795..12842
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg
       61 ttctacaaaa tgaagcacag atgcttcgtt aacaaagata tgctattgaa gtgcaagatg
      121 gaaacgcaga aaatgaaccg gggatgcgac gtgcaagatt acctatgcaa tagatgcaat
      181 agtttctcca ggaaccgaaa tacatacatt gtcttccgta aagcgctaga ctatatatta
      241 ttatacaggt tcaaatatac tatctgtttc agggaaaact cccaggttcg gatgttcaaa
      301 attcaatgat gggtaacaag tacgatcgta aatctgtaaa acagtttgtc ggatattagg
      361 ctgtatctcc tcaaagcgta ttcgaatatc attgagaagc tgcaggcaag tgcacaaaca
      421 atacttaaat aaatactact cagtaataac ctatttctta gcatttttga cgaaatttgc
      481 tattttgtta gagtctttta caccatttgt ctccacacct ccgcttacat caacaccaat
      541 aacgccattt aatctaagcg catcaccaac attttctggc gtcagtccac cagctaacat
      601 aaaatgtaag ctttcggggc tctcttgcct tccaacccag tcagaaatcg agttccaatc
      661 caaaagttca cctgtcccac ctgcttctga atcaaacaag ggaataaacg aatgaggttt
      721 ctgtgaagct gcactgagta gtatgttgca gtcttttgga aatacgagtc ttttaataac
      781 tggcaaaccg aggaactctt ggtattcttg ccacgactca tctccatgca gttggacgat
      841 atcaatgccg taatcattga ccagagccaa aacatcctcc ttaggttgat tacgaaacac
      901 gccaaccaag tatttcggag tgcctgaact atttttatat gcttttacaa gacttgaaat
      961 tttccttgca ataaccgggt caattgttct ctttctattg ggcacacata taatacccag
     1021 caagtcagca tcggaatcta gagcacattc tgcggcctct gtgctctgca agccgcaaac
     1081 tttcaccaat ggaccagaac tacctgtgaa attaataaca gacatactcc aagctgcctt
     1141 tgtgtgctta atcacgtata ctcacgtgct caatagtcac caatgccctc cctcttggcc
     1201 ctctcctttt cttttttcga ccgaattaat tcttgaagac gaaagggcct cgtgatacgc
     1261 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     1321 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1381 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1441 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1501 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1561 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1621 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1681 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1741 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1801 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1861 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1921 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1981 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2041 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2101 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2161 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2221 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2281 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2341 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2401 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2461 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2521 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2581 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     2641 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2701 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2761 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2821 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2881 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2941 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3001 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3061 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3121 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3181 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3241 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     3301 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     3361 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     3421 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3481 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     3541 cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     3601 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     3661 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
     3721 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     3781 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     3841 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
     3901 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
     3961 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
     4021 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
     4081 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
     4141 gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg
     4201 cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat
     4261 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt
     4321 cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc
     4381 ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt
     4441 gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag
     4501 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
     4561 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
     4621 cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc
     4681 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     4741 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     4801 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     4861 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     4921 tctcaagggc atcggtcgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4981 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5041 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5101 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5161 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5221 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5281 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5341 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaaatat
     5401 ttgcttatac aatcttcctg tttttggggc ttttctgatt atcaaccggg gtcgacgctc
     5461 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     5521 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     5581 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     5641 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     5701 gccggccacg atgcgtccgg cgtagaggat cttttatgct tgcttttcaa aaggcctgca
     5761 ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat ttcttagcat
     5821 ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc acacctccgc
     5881 ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt tctggcgtca
     5941 gtccaccagc taacataaaa tgtaagcttt ctaactgatc tatccaaaac tgaaaattac
     6001 attcttgatt aggtttatca caggcaaatg taatttgtgg tattttgccg ttcaaaatct
     6061 gtagaatttt ctcattggtc acattacaac ctgaaaatac tttatctaca atcataccat
     6121 tcttataaca tgtcccctta atactaggat caggcatgaa cgcatcacag acaaaatctt
     6181 cttgacaaac gtcacaattg atccctcccc atccgttatc acaatgacag gtgtcatttt
     6241 gtgctcttat gggacgatcc ttattaccgc tttcatccgg tgatagaccg ccacagaggg
     6301 gcagagagca atcatcacct gcaaaccctt ctatacactc acatctacca gtgtacgaat
     6361 tgcattcaga aaactgtttg cattcaaaaa taggtagcat acaattaaaa catggcgggc
     6421 atgtatcatt gcccttatct tgtgcagtta gacgcgaatt tttcgaagaa gtaccttcaa
     6481 agaatggggt cttatcttgt tttgcaagta ccactgagca ggataataat agaaatgata
     6541 atatactata gtagagataa cgtcgatgac ttcccatact gtaattgctt ttagttgtgt
     6601 atttttagtg tgcaagtttc tgtaaatcga ttaatttttt tttctttcct ctttttatta
     6661 accttaattt ttattttaga ttcctgactt caactcaaga cgcacagata ttataacatc
     6721 tgcataatag gcatttgcaa gaattactcg tgagtaagga aagagtgagg aactatcgca
     6781 tacctgcatt taaagatgcc gatttgggcg cgaatccttt attttggctt caccctcata
     6841 ctattatcag ggccagaaaa aggaagtgtt tccctccttc ttgaattgat gttaccctca
     6901 taaagcacgt ggcctcttat cgagaaagaa attaccgtcg ctcgtgattt gtttgcaaaa
     6961 agaacaaaac tgaaaaaacc cagacacgct cgacttcctg tcttcctatt gattgcagct
     7021 tccaatttcg tcacacaaca aggtcctagc gacggctcac aggttttgta acaagcaatc
     7081 gaaggttctg gaatggcggg aaagggttta gtaccacatg ctatgatgcc cactgtgatc
     7141 tccagagcaa agttcgttcg atcgtactgt tactctctct ctttcaaaca gaattgtccg
     7201 aatcgtgtga caacaacagc ctgttctcac acactctttt cttctaacca agggggtggt
     7261 ttagtttagt agaacctcgt gaaacttaca tttacatata tataaacttg cataaattgg
     7321 tcaatgcaag aaatacatat ttggtctttt ctaattcgta gtttttcaag ttcttagatg
     7381 ctttcttttt ctctttttta cagatcatca aggaagtaat tatctacttt ttacaacaaa
     7441 tctagaattc ggatcctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
     7501 cccgaaaaat ttttaaattc atataactcc ccatgctaag atttgttact aaaaactctc
     7561 aagataaatc gtctgatcta ttctctattt gttctgatcg cggaactttt gttgcccata
     7621 atagagttcg gactgatttc aagtttgaca acttagtatt caaccgagtt tatggtgttt
     7681 ctcaaaaatt tacgttagtc ggtaacccga cagtctgctt taatgaaggc agttcttacc
     7741 tagaaggtat tgctaaaaag tacctaactt tggatggagg acttgccatt gacaatgtcc
     7801 tcaatgagtt gagatccact tgcggtatcc caggtaatgc tgttgcatct catgcgtata
     7861 atattacatc ctggcgttgg tatgacaatc acgtggcact gttgatgaac atgttgcgtg
     7921 cttaccactt acaagtattg accgaacagg gccaatatag cgctggagat atccctatgt
     7981 accatgatgg acatgtcaaa atcaagctac cagtgactat cgatgacacg gcaggcccaa
     8041 cacaattcgc ttggcctagt gacaggtcta ctgattcgta tcctgattgg gcacagtttt
     8101 ctgaatcatt tccatcaatc gacgtcccgt acctagatgt taggccattg accgtaacgg
     8161 aagtcaattt cgtgcttatg atgatgagta agtggcatag acgtactaac ttagcgatag
     8221 actacgaggc accccaacta gctgataagt tcgcttaccg ccatgcgctt actgttcaag
     8281 acgctgacga gtggatagaa ggcgatagaa ctgatgacca gttccgcccc ccctcgtcta
     8341 aagtaatgtt atcggcactt cgtaagtacg tgaaccataa caggctgtac aatcagtttt
     8401 acactgcagc acaactgtta gctcaaatta tgatgaaacc tgtccctaac tgcgctgagg
     8461 gctacgcttg gctgatgcat gacgcattgg tcaatatacc aaaatttggg tctattcgag
     8521 gaaggtaccc ctttttgtta tcaggtgatg cagcgttgat tcaggctaca gccctagaag
     8581 actggtctgc tatcatggcg aaacccgagc tggtgttcac ttacgcgatg caggtgtcag
     8641 tagcgttaaa caccggacta tacttacgtc gcgttaagaa aacaggcttc ggcacaacta
     8701 tagatgacag ctatgaagat ggagcgtttt tgcaaccgga gacgttcgtt caggccgcac
     8761 tagcatgttg taccggacaa gatgcgcccc taaatgggat gtcagatgtg tatgtcactt
     8821 atccagatct tctagaattt gatgctgtta cacaagtacc catcacggtc attgagcccg
     8881 ctggctataa cattgttgat gatcatttag tggttgtggg tgtacctgtg gcatgttcac
     8941 catacatgat atttccagta gctgcgtttg atactgcaaa tccttactgt gggaattttg
     9001 tcattaaggc tgctaacaag tatctccgta agggtgccgt gtatgataaa ctcgaagcat
     9061 ggaagttggc ctgggcactg agggtagccg ggtatgacac tcacttcaaa gtgtatggcg
     9121 atacacacgg cttaactaag ttctatgctg acaacggtga cacatggaca cacatacctg
     9181 aatttgtcac tgacggtgac gtgatggaag tattcgttac tgccatcgaa cgcagagcta
     9241 gacatttcgt tgaactacct agactgaatt caccagcatt cttcagatct gtagaagtca
     9301 gcaccactat atatgatact catgtgcagg ctggtgcgca tgcggtgtat catgctagtc
     9361 gaatcaatct tgattatgtt aagcctgttt cgaccggcat tcaggtgatc aatgcgggcg
     9421 aacttaagaa ctactggggt agtgtgcgtc gtactcagca gggtttagga gtggtaggtc
     9481 ttacgatgcc agctgtaatg cctaccggag aacctacagc tggcgctgcc cacgaagagt
     9541 tgatagaaca ggcggacaat gttttagtag agtaaacgta atcgaaccct cacacggacc
     9601 ccgccctaca aggtacatac tgcaggaacc aggtacgtac cctgcgtgga ttagattcag
     9661 gaacagagta caagctgtat cgcgtcagaa agccactcac ttcttgtttg acatcgtacc
     9721 tgccgcagta attagtgatt ttactacgtc tgacacgtct tcgtttgcat acaaatcgca
     9781 cacctacgct gtaaatgtaa cagcattgag gttcagtgac acttatgcct tgtacgtaca
     9841 gactgatacc aacatgacaa ttttaagccc agcggcgcgt cgccaggctt ctgcgacgta
     9901 ctcacaggtg gcagggtttt gttataacac acctaccgtt atggattcgc tagcgaatat
     9961 cttggacgta gaccgcaata tacgacccaa acacttcaag ggtttacggc tatacacaag
    10021 gtctaaggtc actgctcaac atcatactca cttgcggcca gacgagctag tggaagcggc
    10081 cgcaaaggtc tcgcctagac gtaaatacta cttaatgtgt gtagttgagc tgctcgcgaa
    10141 cttacaagta gatcttgaag cagcagtagc tactattctc gcatatgtcc tgacactaag
    10201 tgaaaaattt gtaccaattt tcttggattc tagagcaata tgggtcggtg agcctgggcc
    10261 tgatgctctg actgcacgtc tcaaggccag tagtgggcag atcaagagca tacacacggc
    10321 tgattacgaa ccactcactg aactattcga gttagcagta ttgatgaacc gaggtgttgg
    10381 ccatgtctct tggcaagctg aaaaggatca tcgcttgaat cccgacgtgg ctgtagttga
    10441 tcaagcacgg ctatattcgt gtgtgcgcga catgttcgaa ggatcaaagc agacgtataa
    10501 atatcccttt atgacgtggg atgactacac tgcaaacaga tgggagtggg ttccaggtgg
    10561 cagtgtccac tctcaatacg aagaagacaa cgattatatc tatcctggtc agtatactag
    10621 gaacaagttc ataactgtta acaaaatgcc caaacacaaa atatctagaa tgatagcatc
    10681 accgcctgag gtacgagctt ggacgtcgac gaagtacgaa tggggcaagc aacgtgctat
    10741 ctacgggacg gatctacgaa gtacactgat aactaacttt gcaatgttca ggtgcgagga
    10801 tgttctcact cacaagttcc cagtaggcga ccaggcagag gcagcaaagg tgcacaaacg
    10861 ggtgaacatg atgctggacg gtgcctctag tttctgcttc gattatgatg acttcaattc
    10921 tcagcattca atagctagta tgtatacggt tttgtgcgct ttcagggaca catttagtcg
    10981 caacatgtct gatgaacaag cagaggcgat gaactgggtg tgtgagtccg tcagacacat
    11041 gtgggtacta gatcctgata ccaaggagtg gtacagacta caaggtacat tactgtcagg
    11101 atggcggtta accacattta tgaacactgt gctaaactgg gcgtatatga aattagctgg
    11161 cgtatttgat ctggatgacg ttcaagactc ggtacacaac ggtgatgatg ttatgattag
    11221 tctcaaccgc gtgagcacag cagtaagaat aatggacgct atgcaccgga taaatgcgcg
    11281 agcacagccg gcgaagtgta acttgttttc gataagtgaa tttctgaggg tagaacacgg
    11341 tatgagcgga ggcgatggtc ttggggctca gtacttaagt aggtcttgtg ctactcttgt
    11401 acacagtagg attgagtcta acgaaccact gtcagtagta cgagttatgg aagcagacca
    11461 ggctagattg cgcgacctgg caaacagaac gcgggtacaa tctgcggtaa cagcgataaa
    11521 agaacaactc gacaaacgtg tcactaagat attcggagtt ggtgatgacg ttgtgcgcga
    11581 catacacaca gctcacaggg tgtgtggcgg tatctcgact gatacctggg caccggttga
    11641 aactaagata ataacagaca atgaagcata tgaaatacca tacgaaatag atgatccatc
    11701 attttggcca ggggtaaatg attatgctta taaagtctgg aaaaatttcg gagaacgact
    11761 cgaatttaat aagattaaag atgccgtagc tagagggagt aggagcacta tagctctgaa
    11821 acgtaaggct aggataacat ctaagaagaa tgaattcgct aacaagtcgg aatgggaaag
    11881 gacaatgtac aaagcctata agggtttggc agtctcatac tatgctaacc tgagcaaatt
    11941 catgagtata ccaccaatgg cgaacattga atttgggcag gctagatatg ctatgcaagc
    12001 agcccttgat agttctgatc cactccgggc attacaggtc atactgtaat tgccaaaaag
    12061 ataatgggaa ttacggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
    12121 aattctgaac cagtcctaaa acgagtaaat aggaccggca attcttcaag caataaacag
    12181 gaataccaat tattaaaaga taacttagtc agatcgtaca ataaagcttt gaagaaaaat
    12241 gcgccttatt caatctttgc tataaaaaat ggcccaaaat ctcacattgg aagacatttg
    12301 atgacctcat ttctttcaat gaagggccta acggagttga ctaatgttgt gggaaattgg
    12361 agcgataagc gtgcttctgc cgtggccagg acaacgtata ctcatcagat aacagcaata
    12421 cctgatcact acttcgcact agtttctcgg tactatgcat atgatccaat atcaaaggaa
    12481 atgatagcat tgaaggatga gactaatcca attgaggagt ggcagcatat agaacagcta
    12541 aagggtagtg ctgaaggaag catacgatac cccgcatgga atgggataat atcacaggag
    12601 gtactagact acctttcatc ctacataaat agacgcatat aagtacgcat ttaagcataa
    12661 acacgcacta tgccgttctt ctcatgtata tatatataca ggcaacacgc agatataggt
    12721 gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt
    12781 ttcggaaacg ctttgaagtt cctattccga agttcctatt ctctagaaag tataggaact
    12841 tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg
    12901 caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa
    12961 aagtatctct ttgctatata tctctgtgct atatccctat ataacctacc catccacctt
    13021 tcgctccttg aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat
    13081 tactctttag acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac
    13141 gaaaatgtaa acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata
    13201 attttctgac caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa
    13261 tccacatcgg tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc
    13321 ttcgctagta atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat
    13381 agacaccaaa gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga
    13441 gactgcatta tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa
    13501 atagcgctct cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta
    13561 aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg
    13621 aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt
    13681 tggtaaaata gcgctttcgc gttgcatttc tgttc
//
