LOCUS       sequence-314538-        9168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 spike protein with a truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang
            Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, 
            Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, 
            Kyratsous CA, Dudkina NV, Shen K, Luban J
  TITLE     Structural and Functional Analysis of the D614G SARS-CoV-2 Spike 
            Protein Variant.
  JOURNAL   Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032.
  PUBMED    32991842
REFERENCE   2  (bases 1 to 9168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032."
COMMENT     Sequence Label: pcDNA3.1 SARS-CoV-2 S D614-trunc
FEATURES             Location/Qualifiers
     source          1..9168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4759..4776)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4765..4989
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5035..5463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5122..5141)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5332..5353
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5472..5492)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5477..5806
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5657..5792
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5719..5738
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5873..6667
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5927..5946)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6537..6556
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6841..6962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6878..6897)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6932..6951
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7011..7027)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7011..7027)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7024..7046)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7035..7051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7059..7065,7066..7083,7084..7089))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7104..7125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7242..7259)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7413..8001)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7493..7512)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8172..8963,8964..9032))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8795..8814
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9033..9137)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga gccaccatgt ttgtgttctt ggtcttgttg cctttggttt
      961 caagtcagtg cgtgaatttg actacacgaa ctcagctgcc gcctgcctac accaattcct
     1021 tcacgcgagg ggtctactac ccagataaag tttttcggag tagtgttctc cactctaccc
     1081 aggacctttt cctccccttc tttagcaacg tcacatggtt ccatgcaata cacgttagcg
     1141 ggacgaatgg gaccaaacgc ttcgataatc cggtactccc gttcaatgat ggggtctact
     1201 tcgcttctac tgagaagtca aacattatcc gcggttggat attcggcaca acacttgatt
     1261 ctaaaactca gtctctcctg attgtgaata atgctaccaa tgttgtgatt aaggtctgcg
     1321 agttccaatt ttgcaatgac ccgttcctgg gcgtttatta tcataagaat aacaagagct
     1381 ggatggagtc tgagttccgc gtatactctt ctgccaataa ttgtaccttc gaatacgtca
     1441 gtcagccgtt cttgatggac ctcgaaggaa agcaaggcaa tttcaaaaac ctcagggagt
     1501 tcgtttttaa gaatatcgat ggttacttta agatatattc aaaacataca cccataaacc
     1561 ttgtccgaga tctgccgcaa ggatttagtg cacttgaacc gctggtcgat cttcctattg
     1621 gaattaacat tacccggttt caaactctgt tggctttgca ccggagttat cttacgccag
     1681 gcgacagctc cagtggatgg acggcagggg ctgctgcata ctatgtcggc taccttcaac
     1741 cccggacttt tctcctcaag tacaacgaga atgggaccat aacagacgct gtagattgtg
     1801 ctctggatcc ccttagcgaa acaaagtgca cgctcaagtc ctttacggtt gaaaaaggga
     1861 tctatcagac cagtaatttc cgcgtacaac ccaccgaaag cattgtacgg tttccgaaca
     1921 ttaccaatct gtgtcctttc ggtgaggttt tcaatgcgac ccggttcgca tcagtctacg
     1981 catggaaccg caagcgcatt agcaattgtg tcgccgatta tagcgtgctt tacaattctg
     2041 cctctttctc cacattcaag tgttatggtg tgtcacctac taagctcaat gacctgtgtt
     2101 ttacaaatgt ttacgccgac agctttgtca ttaggggcga tgaggtccgc cagattgcgc
     2161 ctgggcagac gggaaaaata gccgattata actataagct tcctgatgat tttaccggct
     2221 gtgtgatagc ctggaactca aacaatcttg attctaaagt gggaggaaac tataactatc
     2281 tctaccggct ctttcgcaag tccaacctca aaccgttcga gcgagacata agcacagaaa
     2341 tctaccaagc aggtagtacg ccgtgcaacg gggtagaagg gtttaattgt tacttcccgt
     2401 tgcagagtta cggatttcaa ccaaccaacg gagtagggta tcagccgtac cgcgttgtgg
     2461 ttctctcatt cgaacttctg catgcacccg cgacagtatg tggtccaaag aagtctacga
     2521 acctcgttaa gaataaatgc gtgaacttta actttaatgg cctgacaggg acgggggtcc
     2581 ttaccgagag caacaagaaa tttttgccgt tccagcagtt tgggagggat attgctgaca
     2641 ctaccgacgc agttcgcgac ccacagactc tggaaatact tgacatcaca ccttgctcat
     2701 ttggaggggt ctccgtcata acccctggta ctaacacatc taatcaagtt gctgttctct
     2761 atcaggatgt aaattgtaca gaggttcctg tagccatcca tgctgaccag ctgacaccca
     2821 cctggagggt ttatagtacc ggaagcaacg ttttccaaac tagagcaggt tgtttgattg
     2881 gtgctgagca cgtaaataac tcatacgaat gcgacatacc tattggagcg ggtatctgtg
     2941 caagctacca aactcaaacc aattcaccga ggcgcgcacg atccgttgcg agccaatcca
     3001 taattgccta cacgatgagc ctcggcgcag aaaactcagt tgcgtattcc aataacagca
     3061 ttgcaatccc tacaaatttt acgatcagtg ttacgaccga aatcctgccg gtatcaatga
     3121 cgaaaacaag cgtggactgc accatgtaca tctgtgggga tagtaccgag tgtagtaact
     3181 tgctgctcca gtatgggtca ttttgcactc aactcaacag agctttgaca ggcatcgccg
     3241 ttgaacaaga taaaaatacc caagaagtat ttgctcaagt caaacaaatt tacaagactc
     3301 ctcccataaa agactttgga gggttcaact tttcccagat attgccggac ccatcaaagc
     3361 caagcaagcg gtcatttatt gaagacttgc tcttcaacaa agttacactt gctgacgcgg
     3421 gattcattaa acagtatggt gactgtttgg gagatattgc ggcgcgggac ctgatatgtg
     3481 ctcaaaagtt caatgggctg acggtgctgc ccccgctgct tacggacgaa atgattgccc
     3541 aatacacatc tgctctcctt gccggtacca ttacttcagg gtggacgttc ggcgcaggag
     3601 ctgcccttca aatacctttt gcaatgcaaa tggcatatcg gttcaacgga ataggtgtca
     3661 cgcagaacgt attgtacgag aaccaaaagc tcattgcaaa tcaatttaac tcagcgatcg
     3721 gtaagataca ggactcactt agctctaccg cttccgccct ggggaagctg caagatgtcg
     3781 taaaccagaa cgctcaagcg cttaatacat tggttaaaca attatctagc aattttggtg
     3841 ctatcagttc cgttcttaac gacattttgt ctcggcttga caaggttgag gcggaagttc
     3901 aaatcgacag actcataaca ggacggctgc agtccctgca aacgtatgtc actcaacagc
     3961 tcattcgggc cgcggagatt cgggcaagcg caaaccttgc ggccacaaaa atgagtgaat
     4021 gtgtacttgg ccagtccaag agagttgatt tctgtggaaa aggataccac ctgatgtcct
     4081 tcccgcaatc tgcgccgcac ggcgttgttt ttttgcatgt aacttatgta cctgcgcaag
     4141 aaaaaaattt cacgactgcg cctgcaatat gtcatgacgg gaaggctcac ttcccaaggg
     4201 aaggcgtctt tgtatcaaat gggacgcatt ggttcgtcac gcagcgcaac ttttatgagc
     4261 cccagattat aactaccgac aacacattcg tcagcggcaa ttgtgatgtg gtaatcggca
     4321 tagtcaacaa taccgtatac gatcctttgc aacctgaact tgactcattc aaggaggaat
     4381 tggataagta tttcaaaaat catacctctc cagacgtcga tctcggtgat atatcaggta
     4441 taaatgcgtc agtcgtaaac atccagaagg agattgatcg cttgaacgag gttgctaaga
     4501 atttgaatga aagcctcatc gacctccagg aattagggaa atatgaacaa tacattaagt
     4561 ggccttggta catctggttg gggttcatcg caggactcat cgcgatcgtg atggttacca
     4621 taatgctttg ttgcatgacg agctgttgtt cctgtctgaa aggtaacagg gtgaggcagg
     4681 gctacagcta agaattccac cacactggac tagtggatcc gagctcggta ccaagcttaa
     4741 gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4801 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4861 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4921 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     4981 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     5041 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5101 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5161 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5221 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5281 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5341 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     5401 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5461 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5521 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5581 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     5641 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5701 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     5761 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     5821 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     5881 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5941 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6001 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     6061 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6121 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6181 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6241 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6301 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     6361 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     6421 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6481 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6541 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6601 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6661 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     6721 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     6781 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     6841 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6901 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6961 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7021 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7081 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7141 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7201 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7261 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7321 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7381 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7441 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7501 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7561 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7621 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7681 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7741 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7801 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7861 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7921 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7981 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8041 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8101 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8161 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8221 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8281 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8341 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8401 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8461 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8521 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8581 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8641 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8701 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8761 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8821 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8881 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8941 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9001 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9061 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9121 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
