LOCUS       sequence-314562-        4265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ixodes scapularis NPY-like receptor 1b (NPYLR1b) in a 
            mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Liesch J, Bellani LL, Vosshall LB
  TITLE     Functional and genetic characterization of neuropeptide Y-like 
            receptors in Aedes aegypti.
  JOURNAL   PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2486. doi: 
            10.1371/journal.pntd.0002486. eCollection 2013.
  PUBMED    24130914
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2013-10-10"; volume: "7(10):e2486. doi"; pages: "
            10.1371/journal.pntd.0002486. eCollection 2013"
COMMENT     Sequence Label: pME18s-Ix_NPYLR1b
FEATURES             Location/Qualifiers
     source          1..4265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2153..2287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2190..2209)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2244..2263
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2326..2343)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2497..3085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2577..2596)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3256..4047,4048..4116))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3879..3898
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4117..4221)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4261..4265,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tgaacgtact tctgcagttt ccgctggcgt ccagcttgga
      841 gaatctgagc ctggaggcag aaaatacgag cagccaattt cacaacgtga caagttatca
      901 cgtctcagta atcaatgatt acataacgag cattcccgcc gtgaaagctt tcttctactg
      961 catatacatc ttgatttttg tcgtgggcat ttgcggtaac gtgctcgtgt gttatgtcgt
     1021 attccgaaac aagcccatgc agaccgtgac caacttcttc attacgaact tggggctctc
     1081 ggacattctt ctttgcacgc tggccgttcc tttcacgcca ctatatcagt ttatgcgcaa
     1141 gtgggtgttt ggccgagtac tgtgccacct ggtcccctac gcgcagggcg tcagcgtcta
     1201 catctcctcc ttcaccctta tggccatcgc catcgaccga ttcttcgtga tcatttaccc
     1261 gttcaagccg cggctccaaa taaaagtatg tttcatgatc attgtttgca tctggttgac
     1321 cggagccttg ctgactctgc cctacggcat ctttatgcac cttactccgg atccagatga
     1381 cggtcgacga cactactgcg aagagaagtg gccaggcgaa gagagtcgtc gcaccttcag
     1441 cttttcgaca agcactctgc agtttgttgt tcctttcggt ataatcagtt tttgctacat
     1501 gcgcgtctgc tgcaagcttc gcgaccgcgc acgtgccaag cctggcgcca agtcggtcaa
     1561 gaaagaggag cttgagcgca aacggactag acgcaccaat cgcatgctca tctcgatggt
     1621 ggtcatcttc ggcgcctcct ggctacccct caacttatac aatctcgtca tggacttctt
     1681 tattcaagct gcaagctgga aatacgccaa cgcgttcttc tttctctccc atgccgtcgc
     1741 catgagctcg acctgttaca accctttctt atacacctgg ctcaacgaga acttccgcaa
     1801 ggagttcaag ctggtgctgc cctactttag cgctagtgca cccgctcgtc gcaccaacgg
     1861 ctccaagcca gaccgcacct gttgcaacgg gcgcgaagag gtccaggagt cgttcgtgcc
     1921 tctcgcctcc cagccgccat cgcagcagcc ggccaccgac gacacttcag catctcgcac
     1981 cacgccccca gctcctgctg ttcactacgt atgcgagacg gacaccgtca ggttacacgt
     2041 gtccaacgac tccaaggagg agctgtgagc ggccgctaga ctagtctaga gaaaaaacct
     2101 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2161 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2221 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2281 ctggatcccc gggtaccgag ctcgaattaa ttcctcttcc gcttcctcgc tcactgactc
     2341 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2401 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2461 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2521 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2581 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2641 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2701 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2761 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2821 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2881 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2941 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3001 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3061 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3121 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3361 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3781 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3961 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4021 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4081 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4141 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4201 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcaa gcttggctgt
     4261 ggaat
//
