LOCUS       sequence-314566-       12150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Eukaryotic expression of human SCN2A variant 1 isoform. This channel
            has been modified to be stably maintained in standard bacterial 
            strains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12150)
  AUTHORS   DeKeyser JM, Thompson CH, George AL Jr
  TITLE     Cryptic prokaryotic promoters explain instability of recombinant 
            neuronal sodium channels in bacteria.
  JOURNAL   J Biol Chem. 2021 Jan-Jun;296:100298. doi: 
            10.1016/j.jbc.2021.100298. Epub 2021 Jan 15.
  PUBMED    33460646
REFERENCE   2  (bases 1 to 12150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2021 Jan-Jun;296:100298. doi: 10.1016/j.jbc.2021.100298. Epub 
            2021 Jan 15."
COMMENT     Sequence Label: pIR-CMV-SCN2A-Variant-1-IRES-mScarlet
FEATURES             Location/Qualifiers
     source          1..12150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        614..632
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      664..673
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          5221..5353
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    join(6828..7401,7402..7404,7405..7413)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6995..7012)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7222..7241
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7414..8109
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(7547..7565)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7815..7834
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    8384..8505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8421..8440)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8475..8494
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8512..8967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8649..8670)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8861..8880
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8994..9098
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9100..9291
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9123..9143)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             9438..10232
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9438..10232
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9492..9511)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10102..10121
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10420..10439)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10464..10511
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     repeat_region   10749..10811
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      11120..11708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11609..11628
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   11838..11872
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgatt
      601 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg
      661 gcggccgcca tggcacagtc agtgctggta ccgccaggac ctgacagctt ccgcttcttt
      721 accagggaat cccttgctgc tattgaacaa cgcattgcag aagagaaagc taagagaccc
      781 aaacaggaac gcaaggatga ggatgatgaa aatggcccaa agccaaacag tgacttggaa
      841 gcaggaaaat ctcttccatt tatttatgga gacattcctc cagagatggt gtcagtgccc
      901 ctggaggatc tggaccccta ctatatcaat aagaaaacgt ttatagtatt gaataaaggg
      961 aaagcaatct ctcgattcag tgccacccct gccctttaca ttttaactcc cttcaaccct
     1021 attagaaaat tagctattaa gattttggta cattctttat tcaatatgct cattatgtgc
     1081 acgattctta ccaactgtgt atttatgacc atgagtaacc ctccagactg gacaaagaat
     1141 gtggagtata cctttacagg aatttatact tttgaatcac ttattaaaat acttgcaagg
     1201 ggcttttgtt tagaagattt cacattttta cgggatccat ggaattggtt ggatttcaca
     1261 gtcattactt ttgcatatgt gacagagttt gtggacctgg gcaatgtctc agcgttgaga
     1321 acattcagag ttctccgagc attgaaaaca atttcagtca ttccaggcct gaagaccatt
     1381 gtgggggccc tgatccagtc agtgaagaag ctttctgatg tcatgatctt gactgtgttc
     1441 tgtctaagcg tgtttgcgct aataggattg cagttgttca tgggcaacct acgaaataaa
     1501 tgtttgcaat ggcctccaga taattcttcc tttgaaataa atatcacttc cttctttaac
     1561 aattcattgg atgggaatgg tactactttc aataggacag tgagcatatt taactgggat
     1621 gaatatattg aggataaaag tcacttttat tttttagagg ggcaaaatga tgctctgctt
     1681 tgtggcaaca gctcagatgc aggccagtgt cctgaaggat acatctgtgt gaaggctggt
     1741 agaaacccca actatggcta cacgagcttt gacaccttta gttgggcctt tttgtcctta
     1801 tttcgtctca tgactcaaga cttctgggaa aacctttatc aactgacact acgtgctgct
     1861 gggaaaacgt acatgatatt ttttgtgctg gtcattttct tgggctcatt ctatctaata
     1921 aatttgatct tggctgtggt ggccatggcc tatgaggaac agaatcaggc cacattggaa
     1981 gaggctgaac agaaggaagc tgaatttcag cagatgctcg aacagttgaa aaagcaacaa
     2041 gaagaagctc aggcggcagc tgcagccgca tctgctgaat caagagactt cagtggtgct
     2101 ggtgggatag gagttttttc agagagttct tcagtagcat ctaagttgag ctccaaaagt
     2161 gaaaaagagc tgaaaaacag aagaaagaaa aagaaacaga aagaacagtc tggagaagaa
     2221 gagaaaaatg acagagtccg aaaatcggaa tctgaagaca gcataagaag aaaaggtttc
     2281 cgtttttcct tggaaggaag taggctgaca tatgaaaaga gattttcttc tccacaccag
     2341 tccttactga gcatccgtgg ctcccttttc tctccaagac gcaacagtag ggcgagcctt
     2401 ttcagcttca gaggtcgagc aaaggacatt ggctctgaga atgactttgc tgatgatgag
     2461 cacagcacct ttgaggacaa tgacagccga agagactctc tgttcgtgcc gcacagacat
     2521 ggagaacggc gccacagcaa tgtcagccag gccagccgtg cctccagggt gctccccatc
     2581 ctgcccatga atgggaagat gcatagcgct gtggactgca atggtgtggt ctccctggtc
     2641 gggggccctt ctaccctcac atctgctggg cagctcctac cagagggcac aactactgaa
     2701 acagaaataa gaaagagacg gtccagttct tatcatgttt ccatggattt attggaagat
     2761 cctacatcaa ggcaaagagc aatgagtata gccagtattt tgaccaacac catggaagaa
     2821 cttgaagaat ccagacagaa atgcccacca tgctggtata aatttgctaa tatgtgtttg
     2881 atttgggact gttgtaaacc atggttaaag gtgaaacacc ttgtcaacct ggttgtaatg
     2941 gacccatttg ttgacctggc catcaccatc tgcattgtct taaatacact cttcatggct
     3001 atggagcact atcccatgac ggagcagttc agcagtgtac tgtctgttgg aaacctggtc
     3061 ttcacaggga tcttcacagc agaaatgttt ctcaagataa ttgccatgga tccatattat
     3121 tactttcaag aaggctggaa tatttttgat ggttttattg tgagccttag tttaatggaa
     3181 cttggtttgg caaatgtgga aggattgtca gttctccgat cattccggct gctccgagtt
     3241 ttcaagttgg caaaatcttg gccaactcta aatatgctaa ttaagatcat tggcaattct
     3301 gtgggggctc taggaaacct caccttggta ttggccatca tcgtcttcat ttttgctgtg
     3361 gtcggcatgc agctctttgg taagagctac aaagaatgtg tctgcaagat ttccaatgat
     3421 tgtgaactcc cacgctggca catgcatgac tttttccact ccttcctgat cgtgttccgc
     3481 gtgctgtgtg gagagtggat agagaccatg tgggactgta tggaggtcgc tggccaaacc
     3541 atgtgcctta ctgtcttcat gatggtcatg gtgattggaa atctagtggt tctgaacctc
     3601 ttcttggcct tgcttttgag ttccttcagt tctgacaatc ttgctgccac tgatgatgat
     3661 aacgaaatga ataatctcca gattgctgtg ggaaggatgc agaaaggaat cgattttgtt
     3721 aaaagaaaaa tacgtgaatt tattcagaaa gcctttgtta ggaagcagaa agctttagat
     3781 gaaattaaac cgcttgaaga tctaaataat aaaaaagaca gctgtatttc caaccatacc
     3841 accatagaaa taggcaaaga cctcaattat ctcaaagacg gaaatggaac tactagtggc
     3901 ataggcagca gtgtagaaaa atatgtcgtg gatgaaagtg attacatgtc atttataaac
     3961 aaccctagcc tcactgtgac agtaccaatt gctgttggag aatctgactt tgaaaattta
     4021 aatactgaag aattcagcag cgagtcagat atggaggaaa gcaaagagaa gctaaatgca
     4081 actagttcat ctgaaggcag cacggttgat attggagctc ccgccgaggg agaacagcct
     4141 gaggttgaac ctgaggaatc ccttgaacct gaagcctgtt ttacagaaga ctgtgtacgg
     4201 aagttcaagt gttgtcagat aagcatagaa gaaggcaaag ggaaactctg gtggaatttg
     4261 aggaaaacat gctataagat agtggagcac aattggttcg aaaccttcat tgtcttcatg
     4321 attctgctga gcagtggggc tctggccttt gaagatatat acattgagca gcgaaaaacc
     4381 attaagacca tgttagaata tgctgacaag gttttcactt acatattcat tctggaaatg
     4441 ctgctaaagt gggttgcata tggttttcaa gtgtatttta ccaatgcctg gtgctggcta
     4501 gacttcctga ttgttgatgt ctcactggtt agcttaactg caaatgcctt gggttactca
     4561 gaacttggtg ccatcaaatc cctcagaaca ctaagagctc tgaggccact gagagctttg
     4621 tcccggtttg aaggaatgag ggttgttgta aatgctcttt taggagccat tccatctatc
     4681 atgaatgtac ttctggtttg tctgatcttt tggctaatat tcagtatcat gggagtgaat
     4741 ctctttgctg gcaagtttta ccattgtatt aattacacca ctggagagat gtttgatgta
     4801 agcgtggtca acaactacag tgagtgcaaa gctctcattg agagcaatca aactgccagg
     4861 tggaaaaatg tgaaagtaaa ctttgataac gtaggacttg gatatctgtc tctacttcaa
     4921 gtagccacgt ttaagggatg gatggatatt atgtatgcag ctgttgattc acgaaatgta
     4981 gaattacaac ccaagtatga agacaacctg tacatgtatc tttattttgt catctttatt
     5041 atttttggtt cattctttac cttgaatctt ttcattggtg tcatcataga taacttcaac
     5101 caacagaaaa agaagtttgg aggtcaagac atttttatga cagaagaaca gaagaaatac
     5161 tacaatgcaa tgaaaaaact gggttcaaag aaaccacaaa aacccatacc tcgacctgct
     5221 gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga
     5281 cagagaagac tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc
     5341 tttctctcca cagaacaaat tccaaggaat ggtctttgat tttgtaacca aacaagtctt
     5401 tgatatcagc atcatgatcc tcatctgcct taacatggtc accatgatgg tggaaaccga
     5461 tgaccagagt caagaaatga caaacattct gtactggatt aatctggtgt ttattgttct
     5521 gttcactgga gaatgtgtgc tgaaactgat ctctcttcgt tactactatt tcactattgg
     5581 atggaatatt tttgattttg tggtggtcat tctctccatt gtaggaatgt ttctggctga
     5641 actgatagaa aagtattttg tgtcccctac cctgttccga gtgatccgtc ttgccaggat
     5701 tggccgaatc ctacgtctga tcaaaggagc aaaggggatc cgcacgctgc tctttgcttt
     5761 gatgatgtcc cttcctgcgt tgtttaacat cggcctcctt cttttcctgg tcatgttcat
     5821 ctacgccatc tttgggatgt ccaattttgc ctatgttaag agggaagttg ggatcgatga
     5881 catgttcaac tttgagacct ttggcaacag catgatctgc ctgttccaaa ttacaacctc
     5941 tgctggctgg gatggattgc tagcacctat tcttaatagt ggacctccag actgtgaccc
     6001 tgacaaagat caccctggaa gctcagttaa aggagactgt gggaacccat ctgttgggat
     6061 tttctttttt gtcagttaca tcatcatatc cttcctggtt gtggtgaaca tgtacatcgc
     6121 ggtcatcctg gagaacttca gtgttgctac tgaagaaagt gcagagcctc tgagtgagga
     6181 tgactttgag atgttctatg aggtttggga gaagtttgat cccgatgcga cccagtttat
     6241 agagtttgcc aaactttctg attttgcaga tgccctggat cctcctcttc tcatagcaaa
     6301 acccaacaaa gtccagctca ttgccatgga tctgcccatg gtgagtggtg accggatcca
     6361 ctgtcttgac atcttatttg cttttacaaa gcgtgttttg ggtgagagtg gagagatgga
     6421 tgcccttcga atacagatgg aagagcgatt catggcatca aacccctcca aagtctctta
     6481 tgagcccatt acgaccacgt tgaaacgcaa acaagaggag gtgtctgcta ttattatcca
     6541 gagggcttac agacgctacc tcttgaagca aaaagttaaa aaggtatcaa gtatatacaa
     6601 gaaagacaaa ggcaaagaat gtgatggaac acccatcaaa gaagatactc tcattgataa
     6661 actgaatgag aattcaactc cagagaaaac cgatatgacg ccttccacca cgtctccacc
     6721 ctcgtatgat agtgtgacca aaccagaaaa agaaaaattt gaaaaagaca aatcagaaaa
     6781 ggaagacaaa gggaaagata tcagggaaag taaaaagtaa ggatccgccc ctctccctcc
     6841 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     6901 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     6961 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     7021 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     7081 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     7141 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     7201 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     7261 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     7321 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     7381 tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggcga ggcagtgatc
     7441 aaggagttca tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag
     7501 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     7561 accaagggtg gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc
     7621 tccagggcct tcaccaagca ccccgccgac atccccgact actataagca gtccttcccc
     7681 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgccgt gaccgtgacc
     7741 caggacacct ccctggagga cggcaccctg atctacaagg tgaagctccg cggcaccaac
     7801 ttccctcctg acggccccgt aatgcagaag aagacaatgg gctgggaagc gtccaccgag
     7861 cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta agatggccct gcgcctgaag
     7921 gacggaggcc gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag
     7981 atgcccggcg cctacaacgt cgaccgcaag ttggacatca cctcccacaa cgaggactac
     8041 accgtggtgg aacagtacga acgctccgag ggccgccact ccaccggcgg catggacgag
     8101 ctgtacaagt aacctgcagg aattcccctt cccaacagtt gcgcagcctg aatggcgaat
     8161 ggagatccaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     8221 ttagattcac agtcccaagg ctcatttcag gcccctcagt cctcacagtc tgttcatgat
     8281 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     8341 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     8401 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8461 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa
     8521 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     8581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     8641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     8701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     8761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     8821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     8881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     8941 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     9001 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     9061 cctgataaat gcttcaataa tattgaaaaa ggaagaatcc tgaggcggaa agaaccagct
     9121 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9181 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgccccatgg
     9301 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9361 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     9421 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     9481 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     9541 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     9601 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     9661 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     9721 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     9781 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     9841 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     9901 aggatgatct ggacgaagaa catcaggggc tcgcgccagc cgaactgttc gccaggctca
     9961 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
    10021 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
    10081 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagaa cttggcggcg
    10141 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
    10201 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
    10261 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
    10321 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
    10381 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
    10441 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
    10501 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacg
    10561 atgcgtatgc ctaacgcgtt tttgttactt tatagaagaa attttgagtt tttgtttttt
    10621 tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg
    10681 taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg
    10741 ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac aatatgatta
    10801 tctttctagg gttaaacgcg ttaggcatac gcatcccacc gagaccccat tggggccaat
    10861 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
    10921 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
    10981 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11041 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11101 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11161 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11221 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
    11281 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    11341 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11401 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11461 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11521 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11581 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11641 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11701 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11761 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
    11821 attagttatt aatttaaccc tagaaagata gtctgcgtaa aattgacgca tgcattcttg
    11881 aaatattgct ctctctttct aaatagcgcg aatccgtcgc tgtgcattta ggacatctca
    11941 gtcgccgctt ggagctcccg tgaggcgtgc ttgtcaatgc ggtaagtgtc actgattttg
    12001 aactataacg accgcgtgag tcaaaatgac gcatgattat cttttacgtg acttttaaga
    12061 tttaactcat acgataatta tattgttatt tcatgttcta cttacgtgat aacttattat
    12121 atatatattt tcttgttata gataatgcat
//
