LOCUS       sequence-314568-       10152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cat ACE2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang
            Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, 
            Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, 
            Kyratsous CA, Dudkina NV, Shen K, Luban J
  TITLE     Structural and Functional Analysis of the D614G SARS-CoV-2 Spike 
            Protein Variant.
  JOURNAL   Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032.
  PUBMED    32991842
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032."
COMMENT     Sequence Label: pscALPSpuro-FcACE2 (Cat)
FEATURES             Location/Qualifiers
     source          1..10152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(64..84)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        69..398
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      249..384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     311..330
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        410..789
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        791..989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     944..964
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1007..1187
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1234..1359
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1856..2089
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2273..2317
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2615..2732
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2869..3276
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5725..5754
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6258..6857
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6258..6277)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6754..6774
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6867..7455
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6920..6940)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7338..7349)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7973..8153
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8224..8812)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8304..8323)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8983..9774,9775..9843))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9606..9625
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9844..9948)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10023..10042)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc
       61 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
      121 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
      241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      301 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
      361 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcgcgttg acattgatta
      421 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      481 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      541 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      601 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      661 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      721 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      781 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      841 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      901 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      961 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     1021 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1081 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1141 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1201 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1261 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1321 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1381 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1441 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1501 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1561 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1621 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1681 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     1741 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1801 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1861 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1921 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1981 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     2041 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     2101 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2161 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaattaacaa
     2221 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2281 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2341 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2401 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2461 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2521 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2581 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2641 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2701 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2761 cagtttggtt aattaactgc agccccgata aaataaaaga ttttatttag tctccagaaa
     2821 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt
     2881 tgcaaggcat ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca
     2941 tgaaaatagc taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc
     3001 cggggccaag aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg
     3061 gtccccagat atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct
     3121 cccccaagga cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc
     3181 gcttctgttc gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc
     3241 ggcgcgccag tcctccgaca gactgagtcg cccgggggtc tagaagccgc caccatgtcc
     3301 ggctcatttt ggctgcttct cagttttgct gcactcaccg cggcgcagag cacgacggaa
     3361 gaactcgcga agactttctt ggaaaaattc aatcacgagg ctgaggaatt gtcctatcaa
     3421 agctctcttg caagctggaa ctataataca aacatcacag acgagaatgt tcagaagatg
     3481 aacgaagccg gagcgaaatg gtcagcattc tatgaagagc aatcaaaact cgctaagact
     3541 tacccgcttg cagaaatcca taacaccaca gtaaagcgac aattgcaggc tttgcaacaa
     3601 agtggaagct cagtccttag cgctgataaa tcccagagac ttaacaccat actcaatgca
     3661 atgagcacta tctactcaac gggcaaggct tgcaatccaa ataatccgca agagtgcctg
     3721 ctgcttgagc ctggcttgga tgacattatg gaaaatagca aggattataa tgaaaggttg
     3781 tgggcctggg agggatggcg cgctgaggtc ggaaagcagt tgaggccttt gtacgaggaa
     3841 tatgtagctt tgaaaaacga aatggcgaaa agcaaacaag tcaactacga ggactatggg
     3901 gattattgga gaggcgacta tgaagaagaa tggactgatg gttataacta ttcacgctcc
     3961 caactcatta aggacgtcga gcatacattc acccagatca aaccactgta tcagcacctc
     4021 catgcctacg tcagggcaaa gctgatggat acttaccctt cacgcatttc cccaacgggg
     4081 tgtttgcccg cgcaccttct cggtgacatg tggggaaggt tctggacaaa tctttatcca
     4141 ctgacagtcc cattcggtca gaaaccgaac atagacgtca cggatgcgat ggttaatcag
     4201 tcctgggacg cgcgccggat cttcaaggag gcagagaaat tctttgtctc cgttggactc
     4261 ccgaatatga cgcaaggctt ttgggaaaac tccatgctta cagagccagg cgacagtaga
     4321 aaagtagtct gtcatccaac agcctgggac cttgggaagg gggattttcg catcaagatg
     4381 tgtactaagg ttacgatgga cgactttctt acagcccatc acgaaatggg acacattcag
     4441 tatgatatgg catatgcggt gcagccgttt cttcttagaa acggggccaa tgaaggtttc
     4501 cacgaggctg tcggtgaaat aatgagtttg tccgctgcga cgccgaatca tttgaagacc
     4561 attggtctcc tgagtccagg tttcagtgaa gactcagaga ccgagattaa ctttcttctt
     4621 aaacaggcac tcactattgt aggcactttg ccattcactt atatgctcga aaagtggagg
     4681 tggatggtgt tcaaggggga gataccgaag gaacaatgga tgcagaagtg gtgggagatg
     4741 aaacgagaaa tcgtgggagt ggtagagcca gttccgcacg atgagacata ctgcgacccc
     4801 gctagcctgt tccacgtagc taacgactat agctttatac gatattatac gcgaaccata
     4861 taccagttcc agtttcaaga ggccctttgt agaatagcga agcacgaggg tccactgcac
     4921 aagtgtgaca tatcaaatag ctctgaagct ggaaagaagc tgttgcagat gcttacattg
     4981 ggaaagtcta agccttggac cttggcactg gaacacgtag tgggtgaaaa aaagatgaac
     5041 gtcacgcccc tcctcaaata ttttgaaccg ttgttcacct ggcttaaaga gcaaaaccgg
     5101 aactcttttg ttgggtggaa taccgactgg aggccttacg ctgaccagag tatcaaagtt
     5161 aggattagtt tgaaaagtgc cctcggcgac gaggcgtacg agtggaacga caatgaaatg
     5221 tacttgtttc gctcatccgt agcatacgca atgagagaat atttctccaa agtgaagaat
     5281 caaaccatcc cctttgtcga ggacaacgtg tgggtctcca atctcaaacc gagaatctct
     5341 ttcaatttct ttgtaacagc gtccaaaaac gtcagcgacg tcatcccccg ctctgaggtg
     5401 gaggaagcaa tcaggatgtc aaggtcccgg attaatgacg cattccggct ggacgacaac
     5461 tctcttgagt ttcttggcat acaacctacc ctgtcccccc cgtaccaacc gccggtgaca
     5521 atttggctga tcgtttttgg cgtagtaatg ggcgtggtcg tagttggcat cgtactcctc
     5581 atagtatcag gcattagaaa caggagaaaa aataaccagg cgcggagtga agagaacccg
     5641 tatgccagcg ttgaccttag caagggcgag aacaaccctg gttttcaaca tgcggatgat
     5701 gtccaaacta gtttcggagc agctgagcag aagctgatca gcgaggagga cctgtaggaa
     5761 ttctcgacct tgactggcgg cgcgaccttg aggcctgcgt tcgcctcagt tgccccctct
     5821 gtgcaatggg gagacgcgcc tcatcgcttg acaacggccg aagagccgcc gcgcttccgt
     5881 ctcccgcgtg cgcgcgccat gctgcccacc cccgttccgc actgaccctc ccccgtgccc
     5941 cgcgtcccgt actgccgccc cgccccgagt cccatgccgc agccaccgcg acggagcccg
     6001 caggcgggaa cctgcctccg cgcgttagcg cgcacgcgcg cctcatgtgt cgtccccatc
     6061 agcgccggct tccgtctata ggccagatgc actgtcactc tggcgaagtc gcagacccga
     6121 ttggccggga cggaggcgcg agaccgggtt gcgggcgggg ccgaacgtgg tataaaaggg
     6181 gcgggaggcc aggctcgtgc cgttttgcag acgccaccgc cgaggaaaac cgtgtactat
     6241 tagccacgcg tgccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     6301 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     6361 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     6421 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     6481 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     6541 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     6601 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     6661 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     6721 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     6781 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     6841 gcaagcccgg tgcctgagcg atcgctaatc aacctctgga ttacaaaatt tgtgaaagat
     6901 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6961 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7021 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7081 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7141 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7201 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7261 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7321 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7381 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7441 gggccgcctc cccgcttaat cgcgtcgaga cctagaaaaa catggagcaa tcacaagtag
     7501 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     7561 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     7621 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7681 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     7741 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     7801 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     7861 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     7921 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     7981 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8041 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8101 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     8161 tttcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8221 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8281 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8341 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8401 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8461 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8521 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8581 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8641 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8701 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8761 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8821 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8881 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8941 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9001 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9061 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9121 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9181 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9241 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9301 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9361 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9421 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9481 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9541 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9601 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9661 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9721 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9781 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9841 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9901 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9961 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
    10021 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
    10081 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
    10141 aattgcatga ag
//
