LOCUS       sequence-314581-       10003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TurboID-NES (as control) tagged with 
            N-terminal V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10003)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-2xV5-TurboID-NES-STOP_IDG-K
FEATURES             Location/Qualifiers
     source          1..10003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    173..289
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    323..343
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        343..1554
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1391..1410
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1558..1578)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             1582..1850
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             1860..1901
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    1899..1923
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1926..1935
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1935..1976
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1983..2939
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2946..2972
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     protein_bind    complement(2980..3004)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3034..3622
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3087..3107)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3505..3516)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3636..4135
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3671..3694)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4068..4087
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4156..4755
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4156..4175)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4652..4672
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4893..5126
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5198..5319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5235..5254)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5289..5308
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5359..5494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5514..5533)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5515..5533)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5543..5560)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5543..5559)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5552..5574)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5701..6156
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5788..5807)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5998..6019
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6182..6286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6287..6355,6356..7147)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7318..7906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7807..7826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8060..8077
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8194..8215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8230..8235,8236..8253,8254..8260)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8268..8284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8273..8295
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8292..8308
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8292..8308
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8327..8347
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8329..8347
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8375..8601
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8602..8782
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8829..8954
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9447..9680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9865..9909
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
       61 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
      121 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
      181 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      241 acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta tcgtccagtg
      301 tggtggaatt ctgcagatat cacaactttg tatagaaaag ttggcctccg cgccgggttt
      361 tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
      421 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
      481 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
      541 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
      601 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
      661 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
      721 atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc
      781 cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg
      841 tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt
      901 tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg
      961 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     1021 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     1081 gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg
     1141 cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg
     1201 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     1261 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     1321 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     1381 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     1441 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     1501 tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacggccaa
     1561 cttttgtata caaagtggcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     1621 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     1681 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     1741 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     1801 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc ggcaccatgg
     1861 gtaagcctat ccctaaccct ctcctcggtc tcgattctac aactttgtac aaaaaagcag
     1921 gcttagccac catgggcaag cccatcccca accccctgct gggcctggac agcaccgcta
     1981 gcaaagacaa tactgtgcct ctgaagctga tcgctctcct ggctaatggc gagttccata
     2041 gtggcgaaca gctgggagaa accctgggca tgtccagggc cgctatcaac aagcacattc
     2101 agactctgcg cgactggggc gtggacgtgt tcaccgtgcc cggaaagggc tactctctgc
     2161 ccgagcctat cccgctgctg aacgctaaac agattctggg acagctggac ggcgggagcg
     2221 tggcagtcct gcctgtggtc gactccacca atcagtacct gctggatcga atcggcgagc
     2281 tgaagagtgg ggatgcttgc attgcagaat atcagcaggc agggagagga agcagaggga
     2341 ggaaatggtt ctctcctttt ggagctaacc tgtacctgag tatgttttgg cgcctgaagc
     2401 ggggaccagc agcaatcggc ctgggcccgg tcatcggaat tgtcatggca gaagcgctgc
     2461 gaaagctggg agcagacaag gtgcgagtca aatggcccaa tgacctgtat ctgcaggata
     2521 gaaagctggc aggcatcctg gtggagctgg ccggaataac aggcgatgct gcacagatcg
     2581 tcattggcgc cgggattaac gtggctatga ggcgcgtgga ggaaagcgtg gtcaatcagg
     2641 gctggatcac actgcaggaa gcagggatta acctggacag gaatactctg gccgctacgc
     2701 tgatccgaga gctgcgggca gccctggaac tgttcgagca ggaaggcctg gctccatatc
     2761 tgccacggtg ggagaagctg gataacttca tcaatagacc cgtgaagctg atcattgggg
     2821 acaaagagat tttcgggatt agccggggga ttgataaaca gggagccctg ctgctggaac
     2881 aggacggagt tatcaaaccc tggatgggcg gagaaatcag tctgcggtct gccgaaaagc
     2941 tgcagctgcc tcccctggag cgcctgaccc tggactaaaa cccagctttc ttgtacaaag
     3001 ttgtgatatc cagcacagtg gcggccgctc gacaatcaac ctctggatta caaaatttgt
     3061 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3121 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3181 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3241 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3301 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3361 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3421 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     3481 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3541 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3601 tccctttggg ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag
     3661 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     3721 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     3781 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     3841 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     3901 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     3961 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     4021 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     4081 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     4141 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4201 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     4261 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     4321 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     4381 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     4441 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     4501 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     4561 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     4621 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     4681 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     4741 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     4801 ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga
     4861 tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag
     4921 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     4981 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5041 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5101 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5161 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5221 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5281 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     5341 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5401 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5461 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     5521 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     5581 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     5641 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     5701 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5761 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5821 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5881 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5941 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6001 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6061 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6121 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6181 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6241 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6301 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6361 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6421 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6481 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6541 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6601 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6661 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6721 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6781 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6841 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6901 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6961 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7021 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7081 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7141 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7201 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7261 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7321 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7381 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7441 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7501 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7561 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7621 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7681 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7741 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7801 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7861 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7921 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7981 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8041 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8101 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8161 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8221 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8281 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     8341 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     8401 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     8461 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     8521 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     8581 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     8641 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8701 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8761 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     8821 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     8881 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     8941 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     9001 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     9061 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     9121 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     9181 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     9241 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     9301 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     9361 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     9421 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     9481 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     9541 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     9601 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     9661 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     9721 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     9781 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     9841 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     9901 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     9961 atgatagtag gaggcttggt aggtttaaga atagtttttg ctg
//
