LOCUS       sequence-314583-       10763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of GSK3B (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10763)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-GSK3B_IDG-K
FEATURES             Location/Qualifiers
     source          1..10763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    164..280
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    314..334
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        334..1545
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1382..1401
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1549..1569)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1570..1579
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1582..2352
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2359..2400
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2401..2425
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3248..3259
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3731..3755)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3785..4373
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3838..3858)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4256..4267)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4387..4886
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4422..4445)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4819..4838
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4907..5506
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4907..4926)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5403..5423
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5644..5877
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5949..6070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5986..6005)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6040..6059
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6110..6245
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6265..6284)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6266..6284)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6294..6311)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6294..6310)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6303..6325)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6452..6907
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6539..6558)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6749..6770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6933..7037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7038..7106,7107..7898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8069..8657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8558..8577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8811..8828
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8945..8966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8981..8986,8987..9004,9005..9011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9019..9035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9024..9046
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9043..9059
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9043..9059
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9078..9098
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9080..9098
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9126..9352
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9353..9533
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9580..9705
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10198..10431
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10616..10660
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
       61 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
      121 gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg
      181 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
      241 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgtccagt gtggtggaat
      301 tctgcagata tcacaacttt gtatagaaaa gttggcctcc gcgccgggtt ttggcgcctc
      361 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc
      421 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
      481 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
      541 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
      601 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
      661 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
      721 atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc
      781 gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc
      841 aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc
      901 ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg
      961 gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg
     1021 gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga
     1081 actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc
     1141 cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat
     1201 aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg
     1261 cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg
     1321 gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag
     1381 ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt
     1441 taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt
     1501 aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacggcca acttttgtat
     1561 acaaagtggg ccaccatggg aatcccgctg ctgaacgcta aacagattct gggacagctg
     1621 gacggcggga gcgtggcagt cctgcctgtg gtcgactcca ccaatcagta cctgctggat
     1681 cgaatcggcg agctgaagag tggggatgct tgcattgcag aatatcagca ggcagggaga
     1741 ggaagcagag ggaggaaatg gttctctcct tttggagcta acctgtacct gagtatgttt
     1801 tggcgcctga agcggggacc agcagcaatc ggcctgggcc cggtcatcgg aattgtcatg
     1861 gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc caatgacctg
     1921 tatctgcagg atagaaagct ggcaggcatc ctggtggagc tggccggaat aacaggcgat
     1981 gctgcacaga tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt ggaggaaagc
     2041 gtggtcaatc agggctggat cacactgcag gaagcaggga ttaacctgga caggaatact
     2101 ctggccgcta tgctgatccg agagctgcgg gcagccctgg aactgttcga gcaggaaggc
     2161 ctggctccat atctgtcacg gtgggagaag ctggataact tcatcaatag acccgtgaag
     2221 ctgatcattg gggacaaaga gattttcggg attagccggg ggattgataa acagggagcc
     2281 ctgctgctgg aacaggacgg agttatcaaa ccctggatgg gcggagaaat cagtctgcgg
     2341 tctgccgaaa aggctagcgg caagcccatc cccaaccccc tgctgggcct ggacagcacc
     2401 acaactttgt acaaaaaagt tggcgccacc atgtcagggc ggcccagaac cacctccttt
     2461 gcggagagct gcaagccggt gcagcagcct tcagcttttg gcagcatgaa agttagcaga
     2521 gacaaggacg gcagcaaggt gacaacagtg gtggcaactc ctgggcaggg tccagacagg
     2581 ccacaagaag tcagctatac agacactaaa gtgattggaa atggatcatt tggtgtggta
     2641 tatcaagcca aactttgtga ttcaggagaa ctggtcgcca tcaagaaagt attgcaggac
     2701 aagagattta agaatcgaga gctccagatc atgagaaagc tagatcactg taacatagtc
     2761 cgattgcgtt atttcttcta ctccagtggt gagaagaaag atgaggtcta tcttaatctg
     2821 gtgctggact atgttccgga aacagtatac agagttgcca gacactatag tcgagccaaa
     2881 cagacgctcc ctgtgattta tgtcaagttg tatatgtatc agctgttccg aagtttagcc
     2941 tatatccatt cctttggaat ctgccatcgg gatattaaac cgcagaacct cttgttggat
     3001 cctgatactg ctgtattaaa actctgtgac tttggaagtg caaagcagct ggtccgagga
     3061 gaacccaatg tttcgtatat ctgttctcgg tactataggg caccagagtt gatctttgga
     3121 gccactgatt atacctctag tatagatgta tggtctgctg gctgtgtgtt ggctgagctg
     3181 ttactaggac aaccaatatt tccaggggat agtggtgtgg atcagttggt agaaataatc
     3241 aaggtcctgg gaactccaac aagggagcaa atcagagaaa tgaacccaaa ctacacagaa
     3301 tttaaattcc ctcaaattaa ggcacatcct tggactaagg attcgtcagg aacaggacat
     3361 ttcacctcag gagtgcgggt cttccgaccc cgaactccac cggaggcaat tgcactgtgt
     3421 agccgtctgc tggagtatac accaactgcc cgactaacac cactggaagc ttgtgcacat
     3481 tcattttttg atgaattacg ggacccaaat gtcaaactac caaatgggcg agacacacct
     3541 gcactcttca acttcaccac tcaagaactg tcaagtaatc cacctctggc taccatcctt
     3601 attcctcctc atgctcggat tcaagcagct gcttcaaccc ccacaaatgc cacagcagcg
     3661 tcagatgcta atactggaga ccgtggacag accaataatg ctgcttctgc atcagcttcc
     3721 aactccacct gaccaacttt cttgtacaaa gttgtgatat ccagcacagt ggcggccgct
     3781 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3841 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3901 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3961 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4021 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4081 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4141 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     4201 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4261 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4321 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     4381 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     4441 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     4501 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     4561 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     4621 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     4681 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     4741 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     4801 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     4861 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa
     4921 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     4981 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     5041 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5101 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5161 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5221 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5281 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5341 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5401 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5461 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     5521 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt
     5581 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5641 gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5701 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5761 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5821 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     5881 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     5941 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6001 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6061 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     6121 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6181 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6241 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     6301 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6361 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6421 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     6481 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6541 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6601 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6661 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6721 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6781 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6841 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     6901 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6961 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7021 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7081 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7141 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7201 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7261 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7321 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7381 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7441 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7501 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7561 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7621 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7681 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7741 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7801 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7861 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7921 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7981 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8041 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8461 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8641 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8701 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8761 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8821 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8881 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     8941 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9001 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     9061 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     9121 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     9181 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     9241 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     9301 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     9361 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9421 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9481 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     9541 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     9601 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     9661 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     9721 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     9781 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     9841 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     9901 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     9961 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    10021 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
    10081 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
    10141 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
    10201 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    10261 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    10321 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    10381 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    10441 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    10501 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    10561 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    10621 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    10681 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    10741 aatagttttt gctgtacttt cta
//
