LOCUS       sequence-314601-       10970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CAMKV (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10970)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CAMKV_IDG-K
FEATURES             Location/Qualifiers
     source          1..10970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    72..188
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    222..242
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        242..1453
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1290..1309
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1457..1477)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1478..1487
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1490..2260
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2267..2308
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2309..2333
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3846..3870)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3900..4488
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3953..3973)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4371..4382)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4502..5001
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4537..4560)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4934..4953
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5022..5621
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5022..5041)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5518..5538
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5759..5992
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6064..6185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6101..6120)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6155..6174
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6225..6360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6380..6399)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6381..6399)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6409..6426)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6409..6425)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6418..6440)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6567..7022
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6654..6673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6864..6885
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7048..7152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7153..7221,7222..8013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8184..8772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8673..8692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8926..8943
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9060..9081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9096..9101,9102..9119,9120..9126)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9134..9150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9139..9161
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9158..9174
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9158..9174
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9193..9213
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9195..9213
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9241..9467
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9468..9648
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9695..9820
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10313..10546
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10731..10775
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
       61 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
      121 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc
      181 aaaattttat cgtccagtgt ggtggaattc tgcagatatc acaactttgt atagaaaagt
      241 tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct
      301 gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca
      361 gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta
      421 ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag
      481 gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga
      541 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg
      601 tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc
      661 tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg
      721 ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc
      781 gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg
      841 aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct
      901 tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc
      961 tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag
     1021 ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt
     1081 cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt
     1141 gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa
     1201 tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc
     1261 ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg
     1321 gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc
     1381 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
     1441 ttttttgtta gacggccaac ttttgtatac aaagtgggcc accatgggaa tcccgctgct
     1501 gaacgctaaa cagattctgg gacagctgga cggcgggagc gtggcagtcc tgcctgtggt
     1561 cgactccacc aatcagtacc tgctggatcg aatcggcgag ctgaagagtg gggatgcttg
     1621 cattgcagaa tatcagcagg cagggagagg aagcagaggg aggaaatggt tctctccttt
     1681 tggagctaac ctgtacctga gtatgttttg gcgcctgaag cggggaccag cagcaatcgg
     1741 cctgggcccg gtcatcggaa ttgtcatggc agaagcgctg cgaaagctgg gagcagacaa
     1801 ggtgcgagtc aaatggccca atgacctgta tctgcaggat agaaagctgg caggcatcct
     1861 ggtggagctg gccggaataa caggcgatgc tgcacagatc gtcattggcg ccgggattaa
     1921 cgtggctatg aggcgcgtgg aggaaagcgt ggtcaatcag ggctggatca cactgcagga
     1981 agcagggatt aacctggaca ggaatactct ggccgctatg ctgatccgag agctgcgggc
     2041 agccctggaa ctgttcgagc aggaaggcct ggctccatat ctgtcacggt gggagaagct
     2101 ggataacttc atcaatagac ccgtgaagct gatcattggg gacaaagaga ttttcgggat
     2161 tagccggggg attgataaac agggagccct gctgctggaa caggacggag ttatcaaacc
     2221 ctggatgggc ggagaaatca gtctgcggtc tgccgaaaag gctagcggca agcccatccc
     2281 caaccccctg ctgggcctgg acagcaccac aactttgtac aaaaaagcag gctccaccat
     2341 gccgtttggg tgtgtgactc tgggcgacaa gaagaactat aaccagccat cggaggtgac
     2401 tgacagatat gatttgggac aggtcatcaa gactgaggag ttttgtgaaa tcttccgggc
     2461 caaggacaag acgacaggca agctgcacac ctgcaagaag ttccagaagc gggacggccg
     2521 caaggtgcgg aaagctgcca agaacgagat aggcatcctc aagatggtga agcatcccaa
     2581 catcctacag ctggtggatg tgtttgtgac ccgcaaggag tactttatct tcctggagct
     2641 ggccacgggg agggaggtgt ttgactggat cctggaccag ggctactact cggagcgaga
     2701 cacaagcaac gtggtacggc aagtcctgga ggccgtggcc tatttgcact cactcaagat
     2761 cgtgcacagg aatctcaagc tggagaacct ggtttactac aaccggctga agaactcgaa
     2821 gattgtcatc agtgacttcc atctggctaa gctagaaaat ggcctcatca aggagccctg
     2881 tgggaccccc gagtatctgg ccccagaggt ggtaggccgg cagcggtatg gacgccctgt
     2941 ggactgctgg gccattggag tcatcatgta catcctgctt tcaggcaatc cacctttcta
     3001 tgaggaggtg gaagaagatg attatgagaa ccatgataag aatctcttcc gcaagatcct
     3061 ggctggtgac tatgagtttg actctccata ttgggatgat atttcgcagg cagccaaaga
     3121 cctggtcaca aggctgatgg aggtggagca agaccagcgg atcactgcag aagaggccat
     3181 ctcccatgag tggatttctg gcaatgctgc ttctgataag aacatcaagg atggtgtctg
     3241 tgcccagatt gaaaagaact ttgccagggc caagtggaag aaggctgtcc gagtgaccac
     3301 cctcatgaaa cggctccggg caccagagca gtccagcacg gctgcagccc agtcggcctc
     3361 agccacagac actgccaccc ccggggctgc aggtggggcc acagctgcag ctgcgagtgg
     3421 agctacctca gcccctgagg gtgatgctgc tcgtgctgca aagagtgata atgtggcccc
     3481 cgcagaccgt agtgccaccc cagccacaga tggaagtgcc accccagcca ctgatggcag
     3541 tgtcacccca gccaccgatg gaagcatcac tccagccact gatgggagtg tcaccccagc
     3601 cactgacagg agcgctactc cagccactga tgggagagcc acaccagcca cagaagagag
     3661 cactgtgccc accacccaaa gcagtgccat gctggccacc aaggcagctg ccacccctga
     3721 gccggctatg gcccagccgg acagcacagc cccagagggc gccacaggcc aggctccacc
     3781 ctctagtaaa ggggaagagg ctgctggttg tgcccaggag tctcaaaggg aggaggccag
     3841 ctaggaccca gctttcttgt acaaagttgt gatatccagc acagtggcgg ccgctcgaca
     3901 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3961 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4021 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4081 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4141 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4201 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4261 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     4321 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4381 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4441 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctac
     4501 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     4561 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     4621 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     4681 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     4741 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     4801 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     4861 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     4921 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     4981 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca
     5041 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     5101 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     5161 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5221 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5281 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     5341 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     5401 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     5461 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     5521 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     5581 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     5641 agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt acctttaaga
     5701 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     5761 gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     5821 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5881 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5941 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6001 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6061 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6121 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6181 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     6241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6301 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6361 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     6421 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     6481 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     6541 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     6601 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6661 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6721 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6781 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6841 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6901 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6961 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     7021 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7081 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7141 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7201 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7261 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7321 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7381 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7441 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7501 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7561 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7621 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7681 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7741 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7801 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7861 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7921 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7981 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8041 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8101 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8521 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8581 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8641 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8701 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8761 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8821 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8881 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8941 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     9001 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9061 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9121 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     9181 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     9241 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     9301 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     9361 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     9421 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     9481 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9541 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9601 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     9661 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     9721 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     9781 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     9841 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     9901 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     9961 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
    10021 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
    10081 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
    10141 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
    10201 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
    10261 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
    10321 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
    10381 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
    10441 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
    10501 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
    10561 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
    10621 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
    10681 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
    10741 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
    10801 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    10861 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    10921 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
//
