LOCUS       sequence-314602-       10319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK10 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10319)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK10_IDG-K
FEATURES             Location/Qualifiers
     source          1..10319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    135..251
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    285..305
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        305..1516
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1353..1372
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1520..1540)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1541..1550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1553..2323
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2330..2371
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2372..2396
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3258..3282)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3312..3900
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3365..3385)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3783..3794)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3914..4413
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3949..3972)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4346..4365
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4434..5033
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4434..4453)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4930..4950
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5171..5404
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5476..5597
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5513..5532)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5567..5586
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5637..5772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5792..5811)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5793..5811)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5821..5838)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5821..5837)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5830..5852)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5979..6434
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6066..6085)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6276..6297
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6460..6564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6565..6633,6634..7425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7596..8184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8085..8104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8338..8355
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8472..8493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8508..8513,8514..8531,8532..8538)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8546..8562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8551..8573
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8570..8586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8570..8586
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8605..8625
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8607..8625
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8653..8879
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8880..9060
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9107..9232
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9725..9958
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10143..10187
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
       61 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
      121 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      181 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      241 ttcaaaattt tatcgtccag tgtggtggaa ttctgcagat atcacaactt tgtatagaaa
      301 agttggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc
      361 gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg
      421 acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt
      481 ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc
      541 gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc
      601 cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt
      661 gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg
      721 ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga
      781 ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg
      841 agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct
      901 gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg
      961 ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga
     1021 ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg
     1081 cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg
     1141 tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg
     1201 tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct
     1261 gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg
     1321 gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc
     1381 ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg
     1441 gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt
     1501 ggcttttttg ttagacggcc aacttttgta tacaaagtgg gccaccatgg gaatcccgct
     1561 gctgaacgct aaacagattc tgggacagct ggacggcggg agcgtggcag tcctgcctgt
     1621 ggtcgactcc accaatcagt acctgctgga tcgaatcggc gagctgaaga gtggggatgc
     1681 ttgcattgca gaatatcagc aggcagggag aggaagcaga gggaggaaat ggttctctcc
     1741 ttttggagct aacctgtacc tgagtatgtt ttggcgcctg aagcggggac cagcagcaat
     1801 cggcctgggc ccggtcatcg gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga
     1861 caaggtgcga gtcaaatggc ccaatgacct gtatctgcag gatagaaagc tggcaggcat
     1921 cctggtggag ctggccggaa taacaggcga tgctgcacag atcgtcattg gcgccgggat
     1981 taacgtggct atgaggcgcg tggaggaaag cgtggtcaat cagggctgga tcacactgca
     2041 ggaagcaggg attaacctgg acaggaatac tctggccgct atgctgatcc gagagctgcg
     2101 ggcagccctg gaactgttcg agcaggaagg cctggctcca tatctgtcac ggtgggagaa
     2161 gctggataac ttcatcaata gacccgtgaa gctgatcatt ggggacaaag agattttcgg
     2221 gattagccgg gggattgata aacagggagc cctgctgctg gaacaggacg gagttatcaa
     2281 accctggatg ggcggagaaa tcagtctgcg gtctgccgaa aaggctagcg gcaagcccat
     2341 ccccaacccc ctgctgggcc tggacagcac cacaactttg tacaaaaaag caggctccac
     2401 catggacaag gagaaggatg gcatccccat cagcagcttg cgggagatca cgctgctgct
     2461 ccgcctgcgt catccgaaca tcgtggagct gaaggaggtg gttgtgggga accacctgga
     2521 gagcatcttc ctggtgatgg gttactgtga gcaggacctg gccagcctcc tggagaatat
     2581 gccaacaccc ttctcggagg ctcaggtcaa gtgcatcgtg ctgcaggtgc tccggggcct
     2641 ccagtatctg cacaggaact tcattatcca cagggacctg aaggtttcca acttgctcat
     2701 gaccgacaag ggttgtgtga agacagcgga tttcggcctg gcccgggcct atggtgtccc
     2761 agtaaagcca atgaccccca aggtggtcac tctctggtac cgagcccctg aactgctgtt
     2821 gggaaccacc acgcagacca ccagcatcga catgtgggct gtgggctgca tactggccga
     2881 gctgctggcg cacaggcctc ttctccccgg cacttccgag atccaccaga tcgacttgat
     2941 cgtgcagctg ctgggcacgc ccagtgagaa catctggccg ggcttttcca agctgccact
     3001 ggtcggccag tacagcctcc ggaagcagcc ctacaacaac ctgaagcaca agttcccatg
     3061 gctgtcggag gccgggctgc gcctgctgca cttcctgttc atggcgacgg ccggggactg
     3121 cctggagagc tcctatttca aggagaagcc cctaccctgt gagccggagc tcatgccgac
     3181 ctttccccac caccgcaaca agcgggccgc cccagccacc tccgagggcc agagcaagcg
     3241 ctgtaaaccc tgaatccacc cagctttctt gtacaaagtt gtgatatcca gcacagtggc
     3301 ggccgctcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3361 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3421 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3481 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     3541 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3601 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3661 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     3721 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3781 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3841 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3901 ctggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     3961 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     4021 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     4081 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     4141 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     4201 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     4261 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     4321 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     4381 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg
     4441 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     4501 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     4561 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     4621 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     4681 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     4741 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     4801 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     4861 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     4921 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     4981 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     5041 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatct cgagggcccg
     5101 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5161 aaggggggac tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt
     5221 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5281 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5341 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5401 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     5461 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5521 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5581 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     5641 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5701 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5761 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     5821 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     5881 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     5941 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     6001 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6061 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6121 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6181 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6241 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6301 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6361 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     6421 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6481 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6541 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6601 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6661 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6721 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6781 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6841 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6901 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6961 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     7021 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7081 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7141 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7201 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7261 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7321 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7381 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7441 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7501 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7561 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7621 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7681 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7741 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7801 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7861 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7921 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7981 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     8041 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8101 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8161 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8221 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8281 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8341 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     8401 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     8461 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     8521 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     8581 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     8641 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     8701 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     8761 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     8821 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     8881 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8941 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9001 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9061 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     9121 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     9181 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     9241 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     9301 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     9361 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     9421 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     9481 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     9541 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     9601 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     9661 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     9721 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     9781 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     9841 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     9901 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     9961 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
    10021 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
    10081 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
    10141 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
    10201 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
    10261 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattca
//
