LOCUS       sequence-314605-       10412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDKL4 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10412)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDKL4_IDG-K
FEATURES             Location/Qualifiers
     source          1..10412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    110..226
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    260..280
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        280..1491
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1328..1347
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1495..1515)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1516..1525
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1528..2298
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2305..2346
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2347..2371
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3326..3350)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3380..3968
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3433..3453)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3851..3862)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3982..4481
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4017..4040)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4414..4433
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4502..5101
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4502..4521)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4998..5018
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5239..5472
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5544..5665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5581..5600)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5635..5654
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5705..5840
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5860..5879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5861..5879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5889..5906)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5889..5905)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5898..5920)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6047..6502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6134..6153)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6344..6365
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6528..6632
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6633..6701,6702..7493)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7664..8252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8153..8172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8406..8423
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8540..8561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8576..8581,8582..8599,8600..8606)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8614..8630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8619..8641
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8638..8654
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8638..8654
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8673..8693
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8675..8693
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8721..8947
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8948..9128
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9175..9300
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9793..10026
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10211..10255
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
       61 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
      121 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
      181 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg tccagtgtgg
      241 tggaattctg cagatatcac aactttgtat agaaaagttg gcctccgcgc cgggttttgg
      301 cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca
      361 gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact
      421 cggccttaga accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta
      481 gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg
      541 attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg
      601 gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc
      661 gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc
      721 cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc
      781 gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc
      841 cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg
      901 ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt
      961 attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac
     1021 tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag
     1081 tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg
     1141 gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc
     1201 aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg
     1261 tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt
     1321 aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa
     1381 gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag
     1441 actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga cggccaactt
     1501 ttgtatacaa agtgggccac catgggaatc ccgctgctga acgctaaaca gattctggga
     1561 cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa tcagtacctg
     1621 ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata tcagcaggca
     1681 gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct gtacctgagt
     1741 atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt catcggaatt
     1801 gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa atggcccaat
     1861 gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc cggaataaca
     1921 ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag gcgcgtggag
     1981 gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa cctggacagg
     2041 aatactctgg ccgctatgct gatccgagag ctgcgggcag ccctggaact gttcgagcag
     2101 gaaggcctgg ctccatatct gtcacggtgg gagaagctgg ataacttcat caatagaccc
     2161 gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat tgataaacag
     2221 ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg agaaatcagt
     2281 ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct gggcctggac
     2341 agcaccacaa ctttgtacaa aaaagcaggc tccaccatgg aaaagtatga aaaattagct
     2401 aagactggag aagggtctta tggggttgta ttcaaatgca gaaacaaaac ctctggacaa
     2461 gtagtagctg ttaaaaaatt tgtggaatct gaagatgatc ctgttgttaa gaaaatagca
     2521 ctaagagaaa tacgtatgtt gaagcaatta aaacatccaa atcttgtgaa cctcatcgag
     2581 gtgttcagga gaaaaaggaa aatgcattta gtttttgaat actgtgatca tacactttta
     2641 aatgagctgg aaagaaaccc aaatggagtt gctgatggag tgatcaaaag cgtattatgg
     2701 caaacacttc aagctcttaa tttctgtcat atacataact gtattcacag agatataaaa
     2761 cctgaaaata ttctaataac taagcaagga ataatcaaga tttgtgactt cgggtttgca
     2821 caaattctga ttccaggaga tgcctacacc gattatgtag ctacgagatg gtaccgagct
     2881 cctgaacttc ttgtgggaga tactcagtat ggttcttcag tcgatatatg ggctattggt
     2941 tgtgtttttg cagagctcct gacaggccag ccactgtggc ctggaaaatc agatgtggac
     3001 caactttatc tgataatcag aacactagga aaattaatcc caagacatca atcaatcttt
     3061 aaaagtaacg ggtttttcca tggcatcagt atacctgagc cagaagacat ggaaactctt
     3121 gaggaaaagt tctcagatgt tcatcctgtg gctctgaact tcatgaaggg gtgtctgaag
     3181 atgaatccag atgacagatt aacctgttcc caactcctgg agagctccta ctttgattct
     3241 tttcaagagg cccaaattaa aagaaaagca cgtaatgaag gaagaaacag aagacgccaa
     3301 caggtacttc cgctcaaaag ttaggaccca gctttcttgt acaaagttgt gatatccagc
     3361 acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3421 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3481 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3541 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3601 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3661 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3721 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     3781 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3841 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3901 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3961 ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     4021 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     4081 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     4141 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     4201 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     4261 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     4321 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     4381 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     4441 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     4501 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4561 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4621 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4681 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4741 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4801 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4861 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     4921 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     4981 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     5041 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     5101 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg
     5161 agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5221 taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt
     5281 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5341 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5401 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5461 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     5521 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     5581 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5641 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     5701 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5761 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5821 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     5881 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     5941 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6001 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     6061 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6121 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6181 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6241 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6301 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6361 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6421 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6481 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6541 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6601 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6661 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6721 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6781 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6841 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6901 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6961 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7021 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7081 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7141 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7201 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7261 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7321 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7381 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7441 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7501 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7561 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7621 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7681 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7741 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7801 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7861 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7921 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7981 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8041 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8101 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8161 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8221 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8281 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8341 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8401 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8461 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8521 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8581 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     8641 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     8701 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     8761 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     8821 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     8881 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     8941 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     9001 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9061 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9121 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     9181 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     9241 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     9301 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     9361 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     9421 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     9481 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     9541 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     9601 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     9661 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     9721 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     9781 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     9841 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     9901 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     9961 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
    10021 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
    10081 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
    10141 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
    10201 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
    10261 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
    10321 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
    10381 atattcacca ttatcgtttc agacccacct cc
//
