LOCUS       sequence-314608-       10883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK18 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10883)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK18_IDG-K
FEATURES             Location/Qualifiers
     source          1..10883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    110..226
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    260..280
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        280..1491
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1328..1347
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1495..1515)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1516..1525
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1528..2298
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2305..2346
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2347..2371
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3797..3821)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3851..4439
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3904..3924)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4322..4333)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4453..4952
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4488..4511)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4885..4904
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4973..5572
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4973..4992)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5469..5489
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5710..5943
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6015..6136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6052..6071)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6106..6125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6176..6311
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6331..6350)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6332..6350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6360..6377)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6360..6376)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6369..6391)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6518..6973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6605..6624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6815..6836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6999..7103
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7104..7172,7173..7964)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8135..8723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8624..8643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8877..8894
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9011..9032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9047..9052,9053..9070,9071..9077)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9085..9101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9090..9112
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9109..9125
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9109..9125
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9144..9164
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9146..9164
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9192..9418
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9419..9599
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9646..9771
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10264..10497
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10682..10726
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
       61 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
      121 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
      181 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg tccagtgtgg
      241 tggaattctg cagatatcac aactttgtat agaaaagttg gcctccgcgc cgggttttgg
      301 cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca
      361 gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact
      421 cggccttaga accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta
      481 gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg
      541 attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg
      601 gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc
      661 gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc
      721 cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc
      781 gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc
      841 cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg
      901 ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt
      961 attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac
     1021 tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag
     1081 tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg
     1141 gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc
     1201 aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg
     1261 tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt
     1321 aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa
     1381 gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag
     1441 actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga cggccaactt
     1501 ttgtatacaa agtgggccac catgggaatc ccgctgctga acgctaaaca gattctggga
     1561 cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa tcagtacctg
     1621 ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata tcagcaggca
     1681 gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct gtacctgagt
     1741 atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt catcggaatt
     1801 gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa atggcccaat
     1861 gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc cggaataaca
     1921 ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag gcgcgtggag
     1981 gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa cctggacagg
     2041 aatactctgg ccgctatgct gatccgagag ctgcgggcag ccctggaact gttcgagcag
     2101 gaaggcctgg ctccatatct gtcacggtgg gagaagctgg ataacttcat caatagaccc
     2161 gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat tgataaacag
     2221 ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg agaaatcagt
     2281 ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct gggcctggac
     2341 agcaccacaa gtttgtacaa aaaagcaggc tccaccatga acaagatgaa gaactttaag
     2401 cgccgtttct ccctgtcagt gccccgcact gagaccattg aagaatcctt ggctgaattc
     2461 acggagcaat tcaaccagct ccacaaccgg cggaatgaga acttgcagct cggtcctctt
     2521 ggcagagacc ccccgcagga gtgcagcacc ttctccccaa cagacagcgg ggaggagccg
     2581 gggcagctct cccctggcgt gcagttccag cggcggcaga accagcgccg cttctccatg
     2641 gaggacgtca gcaagaggct ctctctgccc atggatatcc gcctgcccca ggaattccta
     2701 cagaagctac agatggagag cccagatctg cccaagccgc tcagccgcat gtcccgccgg
     2761 gcctccctgt cagacattgg ctttgggaaa ctggaaacat acgtgaaact ggacaaactg
     2821 ggagagggca cctatgccac agtcttcaaa gggcgcagca aactgatgga gaaccttgtg
     2881 gccctgaaag agatccggct ggagcacgag gagggagcgc cctgcactgc catccgagag
     2941 gtgtctctgc tgaagaacct gaagcacgcc aatattgtga ccctgcatga cctcatccac
     3001 acagatcggt ccctcaccct ggtgtttgag tacctggaca gtgacctgaa gcagtatctg
     3061 gaccactgtg ggaacctcat gagcatgcac aacgtcaaga ttttcatgtt ccagctgctc
     3121 cggggcctcg cctactgtca ccaccgcaag atcctgcacc gggacctgaa gccccagaac
     3181 ctgctcatca acgagagggg ggagctgaag ctggccgact ttggactggc cagggccaag
     3241 tcagtgccca caaagactta ctccaatgag gtggtgaccc tgtggtacag gccccccgat
     3301 gtgctgctgg gatccacaga gtactccacc cccattgata tgtggggcgt gggctgcatc
     3361 cactacgaga tggccacagg gaggcccctc ttcccgggct ccacagtcaa ggaggagctg
     3421 cacctcatct ttcgcctcct cgggaccccc acagaagaga cgtggcccgg cgtgaccgcc
     3481 ttctctgagt tccgcaccta cagcttcccc tgctacctcc cgcagccgct catcaaccac
     3541 gcgcccaggt tggatacgga tggcatccac ctcctgagca gcctgctcct gtatgaatcc
     3601 aagagtcgca tgtcagcaga ggctgccctg agtcactcct acttccggtc tctgggagag
     3661 cgtgtgcacc agcttgaaga cactgcctcc atcttctccc tgaaggagat ccagctccag
     3721 aaggacccag gctaccgagg cttggccttc cagcagccag gacgagggaa gaacaggcgg
     3781 cagagcatct tctaggaccc agctttcttg tacaaagtgg tgatatccag cacagtggcg
     3841 gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3901 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3961 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4021 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4081 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4141 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4201 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4261 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4321 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4381 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4441 tggaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     4501 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     4561 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     4621 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     4681 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     4741 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     4801 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     4861 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     4921 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
     4981 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     5041 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     5101 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5161 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     5221 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5281 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     5341 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     5401 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     5461 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     5521 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     5581 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg
     5641 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5701 aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta
     5761 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5821 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5881 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5941 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6001 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6061 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6121 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     6181 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6241 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6301 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     6361 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6421 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6481 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6541 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6601 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6661 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6721 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6781 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6841 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6901 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6961 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7021 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7081 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7141 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7201 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7261 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7321 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7381 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7441 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7501 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7561 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7621 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7681 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7741 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7801 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7861 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7921 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7981 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8041 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8101 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8161 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8221 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8281 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8341 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8401 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8461 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8521 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8581 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8641 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8701 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8761 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8821 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8881 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8941 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     9001 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     9061 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     9121 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     9181 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     9241 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     9301 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     9361 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     9421 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9481 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9541 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9601 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     9661 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     9721 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9781 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9841 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9901 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     9961 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    10021 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    10081 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
    10141 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    10201 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    10261 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    10321 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    10381 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    10441 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    10501 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10561 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    10621 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    10681 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    10741 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    10801 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    10861 attatcgttt cagacccacc tcc
//
