LOCUS       sequence-314610-       10946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDKL2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10946)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDKL2_IDG-K
FEATURES             Location/Qualifiers
     source          1..10946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    65..181
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    215..235
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        235..1446
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1283..1302
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1450..1470)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1471..1480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1483..2253
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2260..2301
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2302..2326
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3815..3839)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3869..4457
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3922..3942)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4340..4351)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4471..4970
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4506..4529)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4903..4922
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4991..5590
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4991..5010)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5487..5507
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5728..5961
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6033..6154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6070..6089)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6124..6143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6194..6329
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6349..6368)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6350..6368)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6378..6395)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6378..6394)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6387..6409)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6536..6991
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6623..6642)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6833..6854
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7017..7121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7122..7190,7191..7982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8153..8741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8642..8661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8895..8912
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9029..9050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9065..9070,9071..9088,9089..9095)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9103..9119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9108..9130
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9127..9143
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9127..9143
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9162..9182
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9164..9182
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9210..9436
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9437..9617
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9664..9789
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10282..10515
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10700..10744
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt
       61 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      121 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt
      181 tatcgtccag tgtggtggaa ttctgcagat atcacaactt tgtatagaaa agttggcctc
      241 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt
      301 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
      361 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
      421 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
      481 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
      541 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
      601 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
      661 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
      721 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
      781 aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt
      841 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
      901 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
      961 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
     1021 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
     1081 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
     1141 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     1201 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     1261 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     1321 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     1381 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     1441 ttagacggcc aacttttgta tacaaagtgg gccaccatgg gaatcccgct gctgaacgct
     1501 aaacagattc tgggacagct ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc
     1561 accaatcagt acctgctgga tcgaatcggc gagctgaaga gtggggatgc ttgcattgca
     1621 gaatatcagc aggcagggag aggaagcaga gggaggaaat ggttctctcc ttttggagct
     1681 aacctgtacc tgagtatgtt ttggcgcctg aagcggggac cagcagcaat cggcctgggc
     1741 ccggtcatcg gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga
     1801 gtcaaatggc ccaatgacct gtatctgcag gatagaaagc tggcaggcat cctggtggag
     1861 ctggccggaa taacaggcga tgctgcacag atcgtcattg gcgccgggat taacgtggct
     1921 atgaggcgcg tggaggaaag cgtggtcaat cagggctgga tcacactgca ggaagcaggg
     1981 attaacctgg acaggaatac tctggccgct atgctgatcc gagagctgcg ggcagccctg
     2041 gaactgttcg agcaggaagg cctggctcca tatctgtcac ggtgggagaa gctggataac
     2101 ttcatcaata gacccgtgaa gctgatcatt ggggacaaag agattttcgg gattagccgg
     2161 gggattgata aacagggagc cctgctgctg gaacaggacg gagttatcaa accctggatg
     2221 ggcggagaaa tcagtctgcg gtctgccgaa aaggctagcg gcaagcccat ccccaacccc
     2281 ctgctgggcc tggacagcac cacaagtttg tacaaaaaag caggctccac catggaaaaa
     2341 tatgaaaacc tgggtttggt tggagaaggg agttatggaa tggtgatgaa gtgtaggaat
     2401 aaagatactg gaagaattgt ggccataaag aagttcttag aaagtgacga tgacaaaatg
     2461 gttaaaaaga ttgcaatgcg agaaatcaag ttactaaagc aacttaggca tgaaaacttg
     2521 gtgaatctct tggaagtgtg taagaaaaaa aaacgatggt acctagtctt tgaatttgtt
     2581 gaccacacaa ttcttgatga cttggagctc tttccaaatg gactagacta ccaagtagtt
     2641 caaaagtatt tgtttcagat tattaatgga attggatttt gtcacagtca caatatcata
     2701 cacagagata taaagccaga gaatatatta gtctcccagt ctggcgttgt caagctatgc
     2761 gattttggat ttgcgcgaac attggcagct cctggggagg tttatactga ttatgtggca
     2821 acccgatggt acagagctcc agaactattg gttggtgatg tcaagtatgg caaggctgtt
     2881 gatgtgtggg ccattggttg tctggtaact gaaatgttca tgggggaacc cctatttcct
     2941 ggagattctg atattgatca gctatatcat attatgatgt gtttaggtaa tctaattcca
     3001 aggcatcagg agctttttaa taaaaatcct gtgtttgctg gagtaaggtt gcctgaaatc
     3061 aaggaaagag aacctcttga aagacgctat cctaagctct ctgaagtggt gatagattta
     3121 gcaaagaaat gcttacatat tgaccccgac aaaagaccct tctgtgctga gctcctacac
     3181 catgatttct ttcaaatgga tggatttgct gagaggtttt cccaagaact acagttaaaa
     3241 gtacagaaag atgccagaaa tgtttcttta tctaaaaaat cccaaaacag aaagaaggaa
     3301 aaagaaaaag atgattcctt agttgaagaa agaaaaacac ttgtggtaca ggataccaat
     3361 gctgatccca aaattaagga ttataaacta tttaaaataa aaggctcaaa aattgatgga
     3421 gaaaaagctg aaaaaggcaa tagagcttca aatgccagct gtctccgtga cagtaggaca
     3481 agccacaaca aaatagtgcc tccaacaagc ctcaaagact gcagcaatgt cagcgtggac
     3541 cacacaagga atccaagcgt ggcaattccc ccacttacac acaatctttc tgcagttgct
     3601 cccagcatta attctggaat ggggactgag actataccaa ttcagggtta cagagtggat
     3661 gagaaaacta agaagtgttc tattccattt gttaaaccga acagacattc cccatcaggc
     3721 atttataaca ttaatgtgac cacattagta tcaggacctc ccctgtcaga tgattcaggg
     3781 gctgatttgc ctcaaatgga acaccagcac taggacccag ctttcttgta caaagtggtg
     3841 atatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag
     3901 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3961 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4021 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4081 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4141 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4201 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4261 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4321 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4381 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4441 ttgggccgcc tccccgcctg gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     4501 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     4561 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     4621 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     4681 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     4741 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     4801 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     4861 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     4921 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     4981 caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     5041 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     5101 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     5161 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     5221 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     5281 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     5341 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     5401 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     5461 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     5521 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     5581 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     5641 tgcatctcga gggcccggta cctttaagac caatgactta caaggcagct gtagatctta
     5701 gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag
     5761 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5821 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5881 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5941 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     6001 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     6061 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6121 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     6181 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6241 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6301 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     6361 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     6421 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     6481 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     6541 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6601 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6661 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6721 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6781 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6841 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6901 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6961 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     7021 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7081 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7141 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7201 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7261 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7321 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     7381 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7441 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7501 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7561 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7621 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7681 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7741 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7801 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7861 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7921 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7981 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8041 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8101 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8161 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8221 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8281 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8341 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8401 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8461 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8521 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8581 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8641 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8701 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8761 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8821 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8881 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8941 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     9001 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     9061 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     9121 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     9181 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     9241 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     9301 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     9361 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     9421 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9481 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9541 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9601 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     9661 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     9721 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     9781 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     9841 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     9901 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     9961 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    10021 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    10081 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
    10141 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
    10201 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
    10261 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
    10321 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
    10381 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
    10441 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
    10501 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
    10561 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
    10621 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
    10681 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
    10741 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
    10801 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
    10861 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
    10921 caggcccgaa ggaatagaag aagaag
//
