LOCUS       sequence-314617-       12254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of DSTYK (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12254)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-DSTYK _IDG-K
FEATURES             Location/Qualifiers
     source          1..12254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    118..234
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    268..288
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        288..1499
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1336..1355
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1503..1523)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1524..1533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1536..2306
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2313..2354
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2355..2379
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5176..5200)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5230..5818
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5283..5303)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5701..5712)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5832..6331
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5867..5890)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6264..6283
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6352..6951
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6352..6371)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6848..6868
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7089..7322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7394..7515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7431..7450)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7485..7504
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7555..7690
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7710..7729)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7711..7729)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7739..7756)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7739..7755)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7748..7770)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7897..8352
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7984..8003)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8194..8215
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8378..8482
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8483..8551,8552..9343)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9514..10102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10003..10022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10256..10273
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10390..10411
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10426..10431,10432..10449,10450..10456)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10464..10480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10469..10491
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10488..10504
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10488..10504
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10523..10543
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10525..10543
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10571..10797
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10798..10978
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11025..11150
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11643..11876
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12061..12105
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
       61 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
      121 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      181 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc
      241 cagtgtggtg gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg
      301 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
      361 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
      421 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
      481 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
      541 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
      601 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
      661 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
      721 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
      781 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
      841 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
      901 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
      961 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     1021 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     1081 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     1141 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     1201 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     1261 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     1321 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     1381 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     1441 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     1501 gccaactttt gtatacaaag tgggccacca tgggaatccc gctgctgaac gctaaacaga
     1561 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
     1621 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
     1681 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
     1741 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
     1801 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
     1861 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
     1921 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     1981 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     2041 tggacaggaa tactctggcc gctatgctga tccgagagct gcgggcagcc ctggaactgt
     2101 tcgagcagga aggcctggct ccatatctgt cacggtggga gaagctggat aacttcatca
     2161 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     2221 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     2281 aaatcagtct gcggtctgcc gaaaaggcta gcggcaagcc catccccaac cccctgctgg
     2341 gcctggacag caccacaagt ttgtacaaaa aagcaggctc caccatggag ggcgacgggg
     2401 tgccatgggg cagcgagccc gtctcgggtc ccggccccgg cggcggcgga atgatccgcg
     2461 agctgtgccg gggcttcggc cgctaccgcc gctacctggg acggctgcga cagaacctgc
     2521 gcgagaccca gaagttcttc cgcgacatca agtgctccca caaccacact tgtctctcct
     2581 ccctcacggg cggcggcggg gccgagcgcg gccctgcagg cgatgtcgcc gaaaccgggc
     2641 tgcaggcggg ccaactgagc tgcatttcct tcccacctaa ggaagagaag tacctccagc
     2701 agattgtgga ctgcctccct tgcatactga tcctcggcca ggattgtaac gtcaagtgcc
     2761 agctgttgaa tctgctgttg ggggtgcagg tgcttcccac caccaagctg ggcagtgagg
     2821 agagctgtaa gcttcggcgc ctccgcttca cctatgggac tcagactcgg gtcagcctgg
     2881 cgctccctgg acagtatgaa ctagtgcaca cgctggttgc tcatcagggc aactgggaga
     2941 ccatccctga ggaggatctg gaggtccaag agaacaatga ggatgctgtt catgttttag
     3001 cggaactgga ggtaacgatg caccatgctc tcttacagga agtggacgtt gtggtagcac
     3061 catgccaagg cctccggccc acagtggatg ttctgggtga cttggtgaat gatttcttgc
     3121 ctgtgataac ctatgcactc cacaaagatg aactctctga gagggatgag caagagcttc
     3181 aggaaatccg aaagtatttc tcctttcctg tattcttttt caaagtgccg aaactgggct
     3241 cggagataat agactcctca accaggagaa tggagagcga aagatcaccg ctttatcgcc
     3301 agctaattga cctgggctat ctgagcagca gtcactggaa ctgtggggct cctggccagg
     3361 atactaaagc tcagagcatg ttggtggaac agagtgaaaa gctgagacac ttgagcacat
     3421 tttctcacca ggtgttacag actcgcctgg tggatgcagc caaggccctg aacctggtgc
     3481 actgccactg ccttgacatc tttattaacc aggcatttga catgcagcgg gacctgcaga
     3541 tcactcccaa acgtctggaa tatactcgaa aaaaggagaa tgagttgtat gagtcattga
     3601 tgaatattgc caaccgaaag caggaggaaa tgaaggatat gattgttgag acacttaata
     3661 ccatgaagga ggaacttctg gatgatgcta ctaacatgga gtttaaagac gtcattgtcc
     3721 ctgagaatgg agaaccagta ggcaccagag agatcaaatg ctgcatccga cagatccagg
     3781 aactcatcat ctcccgactt aatcaggcag tggctaataa gctgatcagc tcagtggatt
     3841 acctgaggga aagcttcgtc ggaaccctgg aacgatgtct gcagagcctg gagaagtctc
     3901 aggatgtctc agttcacatc accagtaatt atctcaaaca gatcttaaat gctgcctatc
     3961 atgttgaagt cacgtttcac tcagggtcgt cagttacaag gatgctatgg gagcaaatca
     4021 aacagatcat ccagcgcatc acatgggtga gcccacctgc catcactctg gaatggaaga
     4081 ggaaggtggc ccaggaagcc attgagagcc tcagcgcctc caaattggct aagagcattt
     4141 gcagccaatt ccggactcgg ctcaatagtt cccacgaggc ttttgcagcc tcctttcggc
     4201 agctggaagc tggccactca ggccggttag agaaaacgga agatctatgg ctgagggttc
     4261 ggaaagatca tgctccccgc ctggcccgcc tttctctgga aagccgttct ttacaggatg
     4321 tcttgcttca tcgtaaacct aaactgggac aggaactggg ccggggccag tatggtgtgg
     4381 tatacctgtg tgacaactgg ggaggacact tcccttgtgc cctcaaatca gttgtccctc
     4441 cagatgagaa gcactggaat gatctggctt tggaatttca ctatatgagg tctctgccga
     4501 agcatgagcg attggtggat ctccatggtt cagtcattga ctacaactat ggtggtggct
     4561 ccagcattgc tgtgctcctc attatggagc ggctacaccg ggatctctac acagggctga
     4621 aggctgggct gaccctggag acacgtttgc agatagcact agatgtggtg gagggaatcc
     4681 gcttcctgca cagccaggga cttgtccatc gtgatatcaa actgaaaaat gtgctgctgg
     4741 ataagcagaa ccgtgccaag atcactgact taggattctg caagccagag gccatgatgt
     4801 caggcagcat tgtggggaca ccaatccata tggcccctga acttttcaca gggaagtacg
     4861 ataattccgt ggatgtctac gcttttggaa ttcttttctg gtatatctgc tcaggctctg
     4921 tcaagctccc tgaggcattt gagaggtgtg ctagcaaaga ccatctctgg aacaatgtgc
     4981 ggaggggggc tcgcccagaa cgtcttcctg tgtttgatga ggagtgctgg cagttgatgg
     5041 aagcctgttg ggatggcgac cccttgaaga ggcctctctt gggcattgtc cagcccatgc
     5101 tccagggcat catgaatcgg ctctgcaagt ccaattctga gcagccaaac agaggactag
     5161 atgattctac ttaggaccca gctttcttgt acaaagtggt gatatccagc acagtggcgg
     5221 ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5281 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5341 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5401 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5461 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5521 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5581 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     5641 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5701 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5761 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     5821 ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     5881 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     5941 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     6001 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     6061 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     6121 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     6181 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     6241 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     6301 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag
     6361 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6421 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6481 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6541 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6601 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     6661 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6721 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6781 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6841 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6901 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
     6961 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt
     7021 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7081 ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac
     7141 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7201 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7261 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7321 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7381 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7441 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7501 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     7561 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7621 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7681 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     7741 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     7801 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     7861 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     7921 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7981 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     8041 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     8101 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     8161 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     8221 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     8281 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     8341 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     8401 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8461 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     8521 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     8581 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     8641 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     8701 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     8761 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8821 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8881 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8941 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     9001 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     9061 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     9121 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     9181 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     9241 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     9301 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     9361 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     9421 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     9481 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     9541 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     9601 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     9661 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     9721 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9781 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9841 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9901 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9961 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    10021 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    10081 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    10141 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    10201 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    10261 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
    10321 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    10381 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    10441 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    10501 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
    10561 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
    10621 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
    10681 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
    10741 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
    10801 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    10861 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    10921 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    10981 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
    11041 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
    11101 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
    11161 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
    11221 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
    11281 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
    11341 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
    11401 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
    11461 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
    11521 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    11581 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    11641 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    11701 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    11761 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    11821 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    11881 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    11941 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    12001 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    12061 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    12121 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    12181 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    12241 ttatcgtttc agac
//
