LOCUS       sequence-31462-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GCN5L2
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             628..645
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(698..716)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      712..759
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(883..902)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt ggcatcatcg agttccatgt
      121 catcggcaac tcactgacgc ccaaggccaa ccggcgggtg ttgctgtggc tcgtggggct
      181 gcagaatgtc ttttcccacc agctgccgcg catgcctaag gagtatatcg cccgcctcgt
      241 ctttgacccg aagcacaaga ctctggcctt gatcaaggat gggcgggtca tcggtggcat
      301 ctgcttccgc atgtttccca cccagggctt cacggagatt gtcttctgtg ctgtcacctc
      361 gaatgagcag gtcaagggtt atgggaccca cctgatgaac cacctgaagg agtatcacat
      421 caagcacaac attctctact tcctcaccta cgccgacgag tacgccatcg gctacttcaa
      481 aaagcagggt ttctccaagg acatcaaggt gcccaagagc cgctacctgg gctacatcaa
      541 ggactacgag ggagcgacgc tgatggagtg tgagctgaat ccccgcatcc cctacacgtg
      601 atgacgaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct
      661 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      721 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      781 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      841 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      901 cttcctttct cgccacgttc gccggc
//
