LOCUS       sequence-314626-       11549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 
            with IFP NLS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA
  TITLE     Engineering synthetic morphogen systems that can program 
            multicellular patterning.
  JOURNAL   Science. 2020 Oct 16;370(6514):327-331. doi: 
            10.1126/science.abc0033.
  PUBMED    33060357
REFERENCE   2  (bases 1 to 11549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-10-16- 16"; volume: "370"; 
            issue: "6514"; pages: "327-331"
COMMENT     Sequence Label: pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP
FEATURES             Location/Qualifiers
     source          1..11549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             249..293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        759..1937
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          990..1928
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1885..1905
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1954..1963
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2011..2040
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2866..2922
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2935..2955
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2959..2979
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2992..3954
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Yu et al., 2014)"
                     /label=IFP2.0
                     /translation="ARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTADG
                     HSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLDW
                     PAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQTV
                     RELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLRL
                     TADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGGQ
                     LWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
     misc_feature    3991..4579
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4044..4064)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4462..4473)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4642..4875
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4900..4918)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4901..4918)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5208..5227)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5561..5583
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5621..5639)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5707..5811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5812..5880,5881..6672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6030..6049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6843..7431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7332..7351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7585..7602
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7672..7692)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7677..8006
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7857..7992
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9140..9205
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9335..9355
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9780..9914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9817..9836)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9871..9890
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10083..10716
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10763..10888
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(11380..11549,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggtc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      601 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      661 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      721 ccagtttgga tcgataagct tgatatcgaa ttccgtgagg ctccggtgcc cgtcagtggg
      781 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
      841 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
      901 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
      961 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     1021 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     1081 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     1141 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     1201 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     1261 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     1321 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     1381 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     1441 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     1501 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     1561 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     1621 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     1681 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     1741 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     1801 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     1861 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     1921 catttcaggt gtcgtgagaa ttctcacgcg gccgccacca tgggcgttaa agtccttttc
     1981 gccctgatct gcattgccgt cgcagaggcc gagcagaaac tcattagtga ggaagatctg
     2041 atggcccagg ttcagctcgt agagtcagga ggccgccttg tccaggcggg ggatagtctc
     2101 aggcttagct gcgctgcaag cggccggact ttcagcacgt ccgctatggc ttggtttagg
     2161 caggcccccg ggagagagcg cgagttcgta gcagccatta cgtggaccgt gggaaacacc
     2221 atcttgggtg acagtgtgaa agggcgattc accatctcaa gggatcgcgc taagaatact
     2281 gttgacctgc aaatggacaa tcttgagccc gaagacaccg ccgtatacta ttgtagcgct
     2341 agaagtcggg gctacgtgtt gtccgtcctc aggtctgtcg actcctacga ttactggggg
     2401 cagggcacgc aagtcacagt gagcggagga ggtggttcag ggggcggggg gagcggtggc
     2461 ggtggcagca tggcacaggt ccagttggta gaatctggcg gcggcctcgt tcaagcgggg
     2521 ggctcacttc gactttcatg tgcggccagc gggcgcacat tttcaaacta cgctatgggc
     2581 tggtttcgac aagcccccgg aaaagagaga gagtttgtgg ctgccataag ctggactggg
     2641 gtgtccacat actacgctga ttccgtaaag ggcaggttca cgataagtag agataatgac
     2701 aaaaataccg tttacgtcca gatgaactcc ctgatcccgg aggacactgc catctactat
     2761 tgcgctgccg ttcgggcccg gagcttttcc gacacttact cacgcgtcaa cgagtacgat
     2821 tactgggggc aggggaccca ggtcacagtt gccagcggca gcggcgccac aaacttctct
     2881 ctgctaaagc aagcaggtga tgttgaagaa aaccccgggc ctcatatggt ggatccaaaa
     2941 aagaagagaa aggtagatcc aaagaagaaa aggaaggttg gcagcggctc cgctcgggac
     3001 cctcaacctt tcttccctcc tctgtatctc ggaggaccag agatcactac tgagaactgc
     3061 gagcgggaac ctatacatat accaggttct attcaacctc atggagcact gctcacagcc
     3121 gatggacatt ctggcgaggt gctgcaagtt tcactcaacg cagccacttt cctgggccag
     3181 gaaccaactg ttctgcgggg acagaccctg gcagccctgc tgccagacca gtggcctgct
     3241 ctgcagactg ccctgcctcc aggctgccag gacgccctgc agtatcgcgc caccctggat
     3301 tggcccgccg ccggacacct gagcctgacc gtgcataggg tggccgagct gctgattctg
     3361 gaattcgagc ctactgaggc atgggactct attggacccc acgctctgag aaatgcaatg
     3421 ttcgctctgg agagtgctcc taatctccgg gcactggctg aagtcgcaac ccaaacagtc
     3481 cgggaactgt caggtttcga ccgggtgatg ctgtacaagt ttgcaccaga cgcaacagga
     3541 gaggttattg ccgaagcaag gcgcgagggc atgcaggctt acctcgggca taggtttccc
     3601 gcatccacca cccctgcaca agctagggcc ctctacacaa gacacctgct ccggctgacc
     3661 gcagacacca gggctgcagc agtgcccctc gaccccgtgc tgaatcccca gacaaatgct
     3721 cctacacctc tgggcggagc tgtcctcaga gctacatccc caatgcacat gcagtacctg
     3781 aggaatatgg gagtgggctc ctccctgagc gtcagcgtcg tggtcggcgg ccagctgtgg
     3841 ggactgattg tctgccacca tcagacaccc tacgtgctgc cacccgatct gcggaccacc
     3901 ctggagtatc tggggaggct cctgtccctg caggtgcaga ggaaagaagc ctgaccgcag
     3961 gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     4021 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4081 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4141 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4201 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4261 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4321 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4381 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4441 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4501 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4561 ctttgggccg cctccccgca tcgataccgt cgaccaaggc agctgtagat cttagccact
     4621 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     4681 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4741 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4801 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4861 ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg
     4921 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta
     4981 caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac
     5041 tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca
     5101 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
     5161 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     5221 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     5281 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     5341 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     5401 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctag ctctgatgcc
     5461 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5521 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5581 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     5641 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5701 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5761 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5821 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5881 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5941 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6001 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6061 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6121 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6181 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6241 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6301 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6361 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6421 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6481 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6541 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6601 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6661 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6721 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6781 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6841 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6901 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6961 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     7021 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7081 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7141 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7201 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7261 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7321 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7381 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7441 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7501 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7561 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7621 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt
     7681 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7741 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7801 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7861 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7921 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7981 tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt
     8041 ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca
     8101 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
     8161 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
     8221 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
     8281 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac
     8341 tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg
     8401 acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg
     8461 ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa
     8521 aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg
     8581 ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg
     8641 tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt
     8701 ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca
     8761 caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg
     8821 ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa
     8881 accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac
     8941 ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac
     9001 gatgatttat acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc
     9061 ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     9121 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     9181 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     9241 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     9301 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     9361 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     9421 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     9481 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     9541 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     9601 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     9661 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     9721 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     9781 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9841 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9901 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
     9961 cataccctat taccactgcc aattacctag tggtttcatt tactctaaac ctgtgattcc
    10021 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    10081 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
    10141 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
    10201 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
    10261 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
    10321 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
    10381 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
    10441 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
    10501 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
    10561 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    10621 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    10681 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
    10741 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
    10801 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
    10861 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
    10921 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
    10981 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
    11041 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
    11101 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
    11161 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
    11221 gcaagcggcc ggtgatcttc agacctggac gatatatatg agggacaatt ggagaagtga
    11281 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
    11341 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
    11401 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
    11461 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
    11521 acagcatctg ttgcaactca cagtctggg
//
