LOCUS       sequence-31465-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: METT10D
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             882..899
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cccctgaata ttttgtttac tttaagaagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcagcggcct ggttccgcgt ggtagtggaa gagtgagcct taattttaaa
      121 gaccccgaag cagtcagagc tctgacgtgt actctcctaa gggaagattt tggactttct
      181 attgatattc cattggagag actaattccc acagttccct tgagactcaa ctatattcac
      241 tgggtagaag atctgatcgg tcaccaggat tctgacaaaa gtactctccg aagaggaatt
      301 gacataggca cgggggcatc ttgcatctac cccttacttg gagcaacctt gaatggctgg
      361 tatttcctcg caacagaagt ggatgatatg tgtttcaact atgcaaagaa aaatgtggaa
      421 cagaataact tatctgatct cataaaagtg gtgaaagtgc cacagaagac actcctgatg
      481 gatgctctta aagaagaatc tgagataatc tatgactttt gcatgtgcaa ccctcccttt
      541 tttgccaatc aattggaagc caagggagta aactcacgaa atcctcgaag acctccgcct
      601 agttctgtta atacaggagg catcacagag atcatggcag aaggaggtga attagagttt
      661 gttaaaagga tcatccatga cagtctacaa cttaaaaaaa gattaagatg gtatagctgc
      721 atgctgggaa agaaatgcag cctggcgcct ctgaaggagg agcttcgcat acaaggggtt
      781 cccaaagtaa cgtacactga attctgtcaa ggtcggacaa tgagatgggc cttagcttgg
      841 agtttttatg attgatgacg aagcttgcgg ccgcactcga gcaccaccac caccaccact
      901 gagatccggc tgcta
//
