LOCUS       sequence-31466-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HSA9761
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 cctctgaata ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca
       61 tcatcatcac agcagcggcc tggttccgcg tggtagtaac acggggattg ggcagcacat
      121 tttgaaaaat cctctcatta ttaacagcat tatcgataag gctgccttaa gaccaactga
      181 tgtagtgctg gaagttggac ctggaactgg caacatgact gtaaagttgt tagaaaaggc
      241 aaaaaaggtt gttgcttgtg aacttgaccc aaggctagta gctgaacttc acaaaagagt
      301 tcagggcacg cctgtggcca gcaaacttca agtactggtg ggtgatgtgc tgaaaacaga
      361 tttgccattc tttgatactt gtgtggcaaa tttgccttat cagatctctt cgccttttgt
      421 cttcaagctg ttgctacatc gacctttttt caggtgtgct atacttatgt ttcaaagaga
      481 atttgccctc cgactggttg caaaacctgg agataagtta tactgcagac tctcaattaa
      541 tacacagctg ttggcacgtg tggaccatct aatgaaagtg ggaaagaata acttcagacc
      601 accgcccaag gtggaatcca gtgttgtaag gatagaacct aagaatccac caccacccat
      661 caattttcag gaatgggatg gtctagtaag gataaccttt gttaggaaaa acaagacact
      721 ctctgctgca tttaaatcaa gtgcagtgca acaactcttg gaaaaaaact acagaattca
      781 ctgttcagtc cataatatta taataccaga agatttcagc atagcagata aaatacagca
      841 aatcctaacc agcacaggtt ttagtgacaa acgggcccgt tccatggaca tagatgactt
      901 catcagattg ctacatgga
//
