LOCUS       sequence-314661-       11027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of DYRK4 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11027)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-DYRK4_IDG-K
FEATURES             Location/Qualifiers
     source          1..11027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    106..222
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    256..276
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        276..1487
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1324..1343
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1491..1511)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1512..1521
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1524..2294
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2301..2342
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2343..2367
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3937..3961)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3991..4579
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4044..4064)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4462..4473)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4593..5092
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4628..4651)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5025..5044
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5113..5712
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5113..5132)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5609..5629
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5850..6083
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6155..6276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6192..6211)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6246..6265
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6316..6451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6471..6490)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6472..6490)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6500..6517)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6500..6516)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6509..6531)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6658..7113
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6745..6764)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6955..6976
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7139..7243
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7244..7312,7313..8104)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8275..8863
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8764..8783
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9017..9034
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9151..9172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9187..9192,9193..9210,9211..9217)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9225..9241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9230..9252
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9249..9265
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9249..9265
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9284..9304
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9286..9304
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9332..9558
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9559..9739
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9786..9911
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10404..10637
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10822..10866
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
       61 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
      121 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
      181 aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgtcca gtgtggtgga
      241 attctgcaga tatcacaact ttgtatagaa aagttggcct ccgcgccggg ttttggcgcc
      301 tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga
      361 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
      421 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
      481 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
      541 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt
      601 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg
      661 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg
      721 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga
      781 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag
      841 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
      901 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
      961 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
     1021 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca
     1081 cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt
     1141 ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga
     1201 cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag
     1261 gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt
     1321 agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt
     1381 tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta
     1441 gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacggc caacttttgt
     1501 atacaaagtg ggccaccatg ggaatcccgc tgctgaacgc taaacagatt ctgggacagc
     1561 tggacggcgg gagcgtggca gtcctgcctg tggtcgactc caccaatcag tacctgctgg
     1621 atcgaatcgg cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga
     1681 gaggaagcag agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt
     1741 tttggcgcct gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca
     1801 tggcagaagc gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc
     1861 tgtatctgca ggatagaaag ctggcaggca tcctggtgga gctggccgga ataacaggcg
     1921 atgctgcaca gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa
     1981 gcgtggtcaa tcagggctgg atcacactgc aggaagcagg gattaacctg gacaggaata
     2041 ctctggccgc tatgctgatc cgagagctgc gggcagccct ggaactgttc gagcaggaag
     2101 gcctggctcc atatctgtca cggtgggaga agctggataa cttcatcaat agacccgtga
     2161 agctgatcat tggggacaaa gagattttcg ggattagccg ggggattgat aaacagggag
     2221 ccctgctgct ggaacaggac ggagttatca aaccctggat gggcggagaa atcagtctgc
     2281 ggtctgccga aaaggctagc ggcaagccca tccccaaccc cctgctgggc ctggacagca
     2341 ccacaagttt gtacaaaaaa gcaggctcca ccatgccggc ctcagagctc aaggcttcag
     2401 aaataccttt ccaccctagc attaaaaccc aggatcccaa ggcagaggag aagtcaccaa
     2461 agaagcaaaa ggtgactctg acagcggcag aggccctaaa gctttttaag aaccagctgt
     2521 ctccatatga acaaagtgaa atcctgggct acgcggagct gtggttcctg ggtcttgaag
     2581 ccaagaagct cgacacggct cctgagaaat ttagcaagac gagttttgat gatgagcatg
     2641 gcttctatct gaaggttctg catgatcaca ttgcctaccg ctatgaagtt ctggagacaa
     2701 tcgggaaggg gtcctttgga caggtggcca agtgcttgga tcacaaaaac aatgagctgg
     2761 tggccctgaa aatcatcagg aacaagaaga ggtttcacca gcaggccctg atggagctga
     2821 agatcctgga agctctcaga aagaaggaca aagacaacac ctacaatgtg gtgcatatga
     2881 aggacttttt ctactttcgc aatcacttct gcatcacctt tgagctcctg ggaatcaact
     2941 tgtatgagtt gatgaagaat aacaactttc aaggcttcag tctgtccata gttcggcgct
     3001 tcactctctc tgttttgaag tgcttgcaga tgctttcggt agagaaaatc attcactgtg
     3061 atctcaagcc cgaaaatata gtgctatacc aaaagggcca agcctctgtt aaagtcattg
     3121 actttggatc aagctgttat gaacaccaga aagtatacac gtacatccaa agccggttct
     3181 accgatcccc agaagtgatc ctgggccacc cctacgacgt ggccattgac atgtggagcc
     3241 tgggctgcat cacggcggag ttgtacacgg gctaccccct gttccccggg gagaatgagg
     3301 tggagcagct ggcctgcatc atggaggtgc tgggtctgcc gccagccggc ttcattcaga
     3361 cagcctccag gagacagaca ttctttgatt ccaaaggttt tcctaaaaat ataaccaaca
     3421 acagggggaa aaaaagatac ccagattcca aggacctcac gatggtgctg aaaacctatg
     3481 acaccagctt cctggacttt ctcagaaggt gtttggtatg ggaaccttct cttcgcatga
     3541 ccccggacca ggccctcaag catgcttgga ttcatcagtc tcggaacctc aagccacagc
     3601 ccaggcccca gaccctgagg aaatccaatt cctttttccc ctctgagaca aggaaggaca
     3661 aggttcaagg ctgtcatcac tcgagcagaa aagcagatga gatcaccaaa gagactacag
     3721 agaaaacaaa agatagcccc acgaagcatg ttcagcattc aggtgatcag caggactgtc
     3781 tccagcacgg agctgacact gttcagctgc ctcaactggt agacgctccc aagaagtcag
     3841 aggcagctgt cggggcggag gtgtccatga cctccccagg acagagcaaa aacttctccc
     3901 tcaagaacac aaacgtttta ccccctattg tataggaccc agctttcttg tacaaagtgg
     3961 tgatatccag cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa
     4021 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4081 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4141 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4201 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4261 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4321 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4381 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4441 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4501 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4561 ctttgggccg cctccccgcc tggaattcta ccgggtaggg gaggcgcttt tcccaaggca
     4621 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     4681 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     4741 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     4801 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     4861 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     4921 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggnng
     4981 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     5041 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     5101 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5161 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5221 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5281 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5341 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5401 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5461 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5521 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5581 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5641 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5701 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     5761 catgcatctc gagggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct
     5821 tagccacttt ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca
     5881 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5941 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6001 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6061 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     6121 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     6181 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6241 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     6301 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6361 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6421 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     6481 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6541 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6601 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     6661 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6721 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6781 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6841 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6901 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6961 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7021 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7081 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     7141 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7201 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7261 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7321 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7381 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7441 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7501 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7561 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7621 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7681 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7741 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7801 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7861 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7921 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7981 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8041 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8101 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8161 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8221 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8281 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8341 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8401 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8461 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8521 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8581 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8641 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8701 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8761 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8821 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8881 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8941 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9001 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9061 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     9121 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     9181 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     9241 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     9301 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     9361 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     9421 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     9481 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     9541 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     9601 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     9661 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     9721 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     9781 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     9841 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     9901 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     9961 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
    10021 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
    10081 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
    10141 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
    10201 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
    10261 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
    10321 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
    10381 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
    10441 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
    10501 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
    10561 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
    10621 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
    10681 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
    10741 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
    10801 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
    10861 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
    10921 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
    10981 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaac
//
