LOCUS       sequence-314662-       10685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PHKG2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10685)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-PHKG2_IDG-K
FEATURES             Location/Qualifiers
     source          1..10685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    115..231
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    265..285
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        285..1496
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1333..1352
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1500..1520)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1521..1530
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1533..2303
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2310..2351
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2352..2376
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3604..3628)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3658..4246
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3711..3731)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4129..4140)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4260..4759
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4295..4318)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4692..4711
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4780..5379
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4780..4799)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5276..5296
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5517..5750
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5822..5943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5859..5878)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5913..5932
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5983..6118
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6138..6157)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6139..6157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6167..6184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6167..6183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6176..6198)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6325..6780
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6412..6431)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6622..6643
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6806..6910
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6911..6979,6980..7771)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7942..8530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8431..8450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8684..8701
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8818..8839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8854..8859,8860..8877,8878..8884)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8892..8908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8897..8919
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8916..8932
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8916..8932
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8951..8971
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8953..8971
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8999..9225
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9226..9406
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9453..9578
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10071..10304
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10489..10533
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
       61 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
      121 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
      181 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgtccag
      241 tgtggtggaa ttctgcagat atcacaactt tgtatagaaa agttggcctc cgcgccgggt
      301 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
      361 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
      421 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
      481 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
      541 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
      601 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
      661 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
      721 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
      781 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
      841 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
      901 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
      961 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
     1021 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
     1081 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
     1141 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     1201 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
     1261 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
     1321 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
     1381 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
     1441 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacggcc
     1501 aacttttgta tacaaagtgg gccaccatgg gaatcccgct gctgaacgct aaacagattc
     1561 tgggacagct ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc accaatcagt
     1621 acctgctgga tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc
     1681 aggcagggag aggaagcaga gggaggaaat ggttctctcc ttttggagct aacctgtacc
     1741 tgagtatgtt ttggcgcctg aagcggggac cagcagcaat cggcctgggc ccggtcatcg
     1801 gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc
     1861 ccaatgacct gtatctgcag gatagaaagc tggcaggcat cctggtggag ctggccggaa
     1921 taacaggcga tgctgcacag atcgtcattg gcgccgggat taacgtggct atgaggcgcg
     1981 tggaggaaag cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg
     2041 acaggaatac tctggccgct atgctgatcc gagagctgcg ggcagccctg gaactgttcg
     2101 agcaggaagg cctggctcca tatctgtcac ggtgggagaa gctggataac ttcatcaata
     2161 gacccgtgaa gctgatcatt ggggacaaag agattttcgg gattagccgg gggattgata
     2221 aacagggagc cctgctgctg gaacaggacg gagttatcaa accctggatg ggcggagaaa
     2281 tcagtctgcg gtctgccgaa aaggctagcg gcaagcccat ccccaacccc ctgctgggcc
     2341 tggacagcac cacaagtttg tacaaaaaag caggctccac catgacgctg gacgtggggc
     2401 cggaggatga gctgcccgac tgggccgccg ccaaagagtt ttaccagaag tacgacccta
     2461 aggacgtcat cggcagagga gtgagctctg tggtccgccg ttgtgttcat cgagctactg
     2521 gccacgagtt tgcggtgaag attatggaag tgacagctga gcggctgagt cctgagcagc
     2581 tggaggaggt gcgggaagcc acacggcgag agacacacat ccttcgccag gtcgccggcc
     2641 acccccacat catcaccctc atcgattcct acgagtcttc tagcttcatg ttcctggtgt
     2701 ttgacctgat gcggaaggga gagctgtttg actatctcac agagaaggtg gccctctctg
     2761 aaaaggaaac caggtccatc atgcggtctc tgctggaagc agtgagcttt ctccatgcca
     2821 acaacattgt gcatcgagat ctgaagcccg agaatattct cctagatgac aatatgcaga
     2881 tccgactttc agatttcggg ttctcctgcc acttggaacc tggcgagaag cttcgagagt
     2941 tgtgtgggac cccagggtat ctagcgccag agatccttaa atgctccatg gatgaaaccc
     3001 acccaggcta tggcaaggag gtcgacctct gggcctgtgg ggtgatcttg ttcacactcc
     3061 tggctggctc gccacccttc tggcaccggc ggcagatcct gatgttacgc atgatcatgg
     3121 agggccagta ccagttcagt tcccccgagt gggatgaccg ttccagcact gtcaaagacc
     3181 tgatctccag gctgctgcag gtggatcctg aggcacgcct gacagctgag caggccctac
     3241 agcacccctt ctttgagcgt tgtgaaggca gccaaccctg gaacctcacc ccccgccagc
     3301 ggttccgggt ggcagtgtgg acagtgctgg ctgctggacg agtggcccta agcacccatc
     3361 gtgtacggcc actgaccaag aatgcactgt tgagggaccc ttatgcgctg cggtcagtgc
     3421 ggcacctcat cgacaactgt gccttccggc tctacgggca ctgggtaaag aaaggggagc
     3481 agcagaaccg ggcggctctc tttcagcacc ggccccctgg gccttttccc atcatgggcc
     3541 ctgaagagga gggagactct gctgctataa ctgaggatga ggccgtgctt gtgctgggct
     3601 aggacccagc tttcttgtac aaagtggtga tatccagcac agtggcggcc gctcgacaat
     3661 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3721 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3781 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3841 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3901 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3961 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4021 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     4081 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4141 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4201 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattctaccg
     4261 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     4321 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     4381 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     4441 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     4501 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     4561 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     4621 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     4681 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     4741 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     4801 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     4861 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     4921 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     4981 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     5041 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     5101 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     5161 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     5221 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     5281 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     5341 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     5401 cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac ctttaagacc
     5461 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5521 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     5581 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5641 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5701 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     5761 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     5821 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5881 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5941 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     6001 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6061 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6121 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     6181 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6241 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6301 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6361 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6421 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6481 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6541 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6601 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6661 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6721 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6781 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6841 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6901 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6961 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7021 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7081 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7141 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7201 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7261 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7321 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7381 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7441 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7501 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7561 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7621 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7681 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7741 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7801 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7861 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8581 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8641 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8701 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8761 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     8821 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     8881 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     8941 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     9001 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     9061 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     9121 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     9181 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     9241 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9301 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9361 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     9421 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     9481 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     9541 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     9601 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     9661 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     9721 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     9781 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     9841 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     9901 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     9961 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    10021 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    10081 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    10141 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    10201 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    10261 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    10321 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    10381 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    10441 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    10501 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    10561 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    10621 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    10681 accca
//
