LOCUS       sequence-314664-       11024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of RIOK3 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11024)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-RIOK3_IDG-K
FEATURES             Location/Qualifiers
     source          1..11024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    104..220
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    254..274
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        274..1485
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1322..1341
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1489..1509)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1510..1519
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1522..2292
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2299..2340
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2341..2365
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3932..3956)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3986..4574
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4039..4059)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4457..4468)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4588..5087
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4623..4646)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5020..5039
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5108..5707
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5108..5127)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5604..5624
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5845..6078
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6150..6271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6187..6206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6241..6260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6311..6446
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6466..6485)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6467..6485)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6495..6512)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6495..6511)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6504..6526)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6653..7108
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6740..6759)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6950..6971
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7134..7238
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7239..7307,7308..8099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8270..8858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8759..8778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9012..9029
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9146..9167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9182..9187,9188..9205,9206..9212)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9220..9236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9225..9247
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9244..9260
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9244..9260
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9279..9299
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9281..9299
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9327..9553
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9554..9734
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9781..9906
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10399..10632
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10817..10861
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
       61 gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg
      121 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
      181 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgtccagt gtggtggaat
      241 tctgcagata tcacaacttt gtatagaaaa gttggcctcc gcgccgggtt ttggcgcctc
      301 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc
      361 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
      421 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
      481 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
      541 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
      601 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
      661 atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc
      721 gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc
      781 aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc
      841 ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg
      901 gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg
      961 gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga
     1021 actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc
     1081 cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat
     1141 aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg
     1201 cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg
     1261 gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag
     1321 ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt
     1381 taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt
     1441 aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacggcca acttttgtat
     1501 acaaagtggg ccaccatggg aatcccgctg ctgaacgcta aacagattct gggacagctg
     1561 gacggcggga gcgtggcagt cctgcctgtg gtcgactcca ccaatcagta cctgctggat
     1621 cgaatcggcg agctgaagag tggggatgct tgcattgcag aatatcagca ggcagggaga
     1681 ggaagcagag ggaggaaatg gttctctcct tttggagcta acctgtacct gagtatgttt
     1741 tggcgcctga agcggggacc agcagcaatc ggcctgggcc cggtcatcgg aattgtcatg
     1801 gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc caatgacctg
     1861 tatctgcagg atagaaagct ggcaggcatc ctggtggagc tggccggaat aacaggcgat
     1921 gctgcacaga tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt ggaggaaagc
     1981 gtggtcaatc agggctggat cacactgcag gaagcaggga ttaacctgga caggaatact
     2041 ctggccgcta tgctgatccg agagctgcgg gcagccctgg aactgttcga gcaggaaggc
     2101 ctggctccat atctgtcacg gtgggagaag ctggataact tcatcaatag acccgtgaag
     2161 ctgatcattg gggacaaaga gattttcggg attagccggg ggattgataa acagggagcc
     2221 ctgctgctgg aacaggacgg agttatcaaa ccctggatgg gcggagaaat cagtctgcgg
     2281 tctgccgaaa aggctagcgg caagcccatc cccaaccccc tgctgggcct ggacagcacc
     2341 acaagtttgt acaaaaaagc aggctccacc atggatctgg taggagtggc atcgcctgag
     2401 cccgggacgg cagcggcctg gggacccagc aagtgtccat gggctattcc tcaaaataca
     2461 atatcttgtt ctttggctga tgtaatgagt gaacagctgg ccaaagaatt gcagttagaa
     2521 gaagaagctg ccgtttttcc tgaagttgct gttgctgaag gaccatttat tactggagaa
     2581 aacattgata cttccagtga ccttatgctg gctcagatgc tacagatgga atatgacaga
     2641 gaatatgatg cacagcttag gcgtgaagaa aaaaaattca atggagatag caaagtttcc
     2701 atttcctttg aaaattatcg aaaagtgcat ccttatgaag acagcgatag ctctgaagat
     2761 gaggttgact ggcaggatac tcgtgatgat ccctacagac cagcaaaacc ggttcccact
     2821 cctaaaaagg gctttattgg aaaaggaaaa gatatcacca ccaaacatga tgaagtagta
     2881 tgtgggagaa agaacacagc aagaatggaa aattttgcac ctgagtttca ggtaggagat
     2941 ggaattggaa tggatttaaa actatcaaac catgttttca atgctttaaa acaacatgcc
     3001 tactcagaag aacgtcgaag tgcccgccta catgagaaaa aggagcattc tacagcagaa
     3061 aaagcagttg atcctaagac acgtttactt atgtataaaa tggtcaactc tggaatgttg
     3121 gagacaatca ctggctgtat tagtacagga aaggagtctg ttgtctttca tgcatatgga
     3181 gggagcatgg aggatgaaaa ggaagatagt aaagttatac ctacagaatg tgccatcaag
     3241 gtatttaaaa caacccttaa tgaatttaag aatcgtgaca aatatattaa agatgatttc
     3301 aggtttaaag atcgcttcag taaactaaat ccacgtaaga tcatccgcat gtgggcagaa
     3361 aaagaaatgc acaatctcac aagaatgcag agagctggaa ttccttgtcc aacagttgta
     3421 ctactgaaga aacacatttt agttatgtct tttattggcc atgatcaagt tccagcccct
     3481 aaattaaaag aagtaaagct caatagtgaa gaaatgaaag aagcctacta tcaaactctt
     3541 catttgatgc ggcagttata tcatgaatgt acgcttgtcc atgctgacct cagtgagtat
     3601 aacatgctgt ggcatgctgg aaaggtctgg ttgatcgatg tcagtcagtc agtagaacct
     3661 acccaccctc acggcctgga gttcttgttc cgggactgca ggaatgtctc gcagtttttc
     3721 cagaaaggag gagtcaagga agcccttagt gaacgagaac tcttcaatgc tgtttcaggc
     3781 ttaaacatca cagcagataa tgaagctgat tttttagctg agatagaagc tttggagaaa
     3841 atgaatgaag atcacgttca gaagaatgga aggaaagctg cttcattttt gaaagatgat
     3901 ggagacccac cactactata tgatgaatag gacccagctt tcttgtacaa agtggtgata
     3961 tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt
     4021 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4081 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4141 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4201 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4261 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4321 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4381 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4441 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4501 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4561 ggccgcctcc ccgcctggaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     4621 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     4681 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     4741 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     4801 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     4861 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     4921 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     4981 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     5041 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa
     5101 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     5161 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5221 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5281 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5341 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5401 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5461 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     5521 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5581 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     5641 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     5701 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
     5761 atctcgaggg cccggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5821 actttttaaa agaaaagggg ggactggaag ggctagctca ctcccaacga agacaagatc
     5881 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5941 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6001 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6061 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6121 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6241 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6301 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6361 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6421 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     6481 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6541 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6601 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     6661 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6721 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6781 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6841 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6901 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6961 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7021 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7081 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     7141 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7201 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7261 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7321 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7381 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7441 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7501 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7561 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7621 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7681 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7741 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7801 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7861 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7921 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7981 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8041 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8101 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8161 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8221 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8281 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8341 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8401 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8461 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8521 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8581 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8641 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8701 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8761 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8821 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8881 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8941 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9001 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9061 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9121 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9181 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9241 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     9301 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     9361 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     9421 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     9481 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     9541 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9601 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9661 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9721 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     9781 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     9841 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     9901 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     9961 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    10021 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    10081 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    10141 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    10201 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
    10261 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    10321 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    10381 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    10441 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    10501 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    10561 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    10621 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    10681 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    10741 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    10801 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    10861 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    10921 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    10981 gcagggatat tcaccattat cgtttcagac ccacctccca accc
//
