LOCUS       sequence-314666-       10979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of VRK2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10979)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-VRK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..10979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    124..240
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    274..294
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        294..1505
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1342..1361
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1509..1529)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1530..1539
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1542..2312
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2319..2360
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2361..2385
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             3528..3539
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(3919..3943)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3973..4561
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4026..4046)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4444..4455)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4575..5062
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4610..4633)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4995..5014
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5083..5682
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5083..5102)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5579..5599
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5820..6053
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6125..6246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6162..6181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6216..6235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6286..6421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6441..6460)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6442..6460)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6470..6487)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6470..6486)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6479..6501)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6628..7083
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6715..6734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6925..6946
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7109..7213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7214..7282,7283..8074)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8245..8833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8734..8753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8987..9004
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9121..9142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9157..9162,9163..9180,9181..9187)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9195..9211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9200..9222
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9219..9235
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9219..9235
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9254..9274
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9256..9274
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9302..9528
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9529..9709
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9756..9881
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10374..10607
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10792..10836
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
       61 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
      121 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      181 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
      241 atcgtccagt gtggtggaat tctgcagata tcacaacttt gtatagaaaa gttggcctcc
      301 gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc
      361 agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg
      421 ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga
      481 cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta
      541 gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata
      601 taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt
      661 cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg
      721 gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc
      781 tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa
      841 atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt
      901 gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat
      961 gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga
     1021 agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg
     1081 gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt
     1141 cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg
     1201 cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg
     1261 cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat
     1321 gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga
     1381 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
     1441 attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt
     1501 tagacggcca acttttgtat acaaagtggg ccaccatggg aatcccgctg ctgaacgcta
     1561 aacagattct gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcca
     1621 ccaatcagta cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag
     1681 aatatcagca ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta
     1741 acctgtacct gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc
     1801 cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag
     1861 tcaaatggcc caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc
     1921 tggccggaat aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta
     1981 tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga
     2041 ttaacctgga caggaatact ctggccgcta tgctgatccg agagctgcgg gcagccctgg
     2101 aactgttcga gcaggaaggc ctggctccat atctgtcacg gtgggagaag ctggataact
     2161 tcatcaatag acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg
     2221 ggattgataa acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg
     2281 gcggagaaat cagtctgcgg tctgccgaaa aggctagcgg caagcccatc cccaaccccc
     2341 tgctgggcct ggacagcacc acaactttgt acaaaaaagc aggctccacc atgccaccaa
     2401 aaagaaatga aaaatacaaa cttcctattc catttccaga aggcaaggtt ctggatgata
     2461 tggaaggcaa tcagtgggta ctgggcaaga agattggctc tggaggattt ggattgatat
     2521 atttagcttt ccccacaaat aaaccagaga aagatgcaag acatgtagta aaagtggaat
     2581 atcaagaaaa tggcccgtta ttttcagaac ttaaatttta tcagagagtt gcaaaaaaag
     2641 actgtatcaa aaagtggata gaacgcaaac aacttgatta tttaggaatt cctctgtttt
     2701 atggatctgg tctgactgaa ttcaagggaa gaagttacag atttatggta atggaaagac
     2761 taggaataga tttacagaag atctcaggcc agaatggtac ctttaaaaag tcaactgtcc
     2821 tgcaattagg tatccgaatg ttggatgtac tggaatatat acatgaaaat gaatatgttc
     2881 atggtgatgt aaaagcagca aatctacttt tgggttacaa aaatccagac caggtttatc
     2941 ttgcagatta tggactttcc tacagatatt gtcccaatgg gaaccacaaa cagtatcagg
     3001 aaaatcctag aaaaggccat aatgggacaa tagagtttac cagcttggat gcccacaagg
     3061 gagtagcctt gtccagacga agtgacgttg agatcctcgg ctactgcatg ctgcggtggt
     3121 tgtgtgggaa acttccctgg gaacagaacc tgaaggaccc tgtggctgtg cagactgcta
     3181 aaacaaatct gttggacgag ctcccccagt cagtgcttaa atgggctcct tctggaagca
     3241 gttgctgtga aatagcccaa tttttggtat gtgctcatag tttagcatat gatgaaaagc
     3301 caaactatca agccctcaag aaaattttga accctcatgg aataccttta ggaccactgg
     3361 acttttccac aaaaggacag agtataaatg tccatactcc aaacagtcaa aaagttgatt
     3421 cacaaaaggc tgcaacaaag caagtcaaca aggcacacaa taggttaatc gaaaaaaaag
     3481 tccacagtga gagaagcgct gagtcctgtg caacatggaa agtgcagaaa gaggagaaac
     3541 tgattggatt gatgaacaat gaagcagctc aggaaagcac aaggagaaga cagaaatatc
     3601 aagagtctca agaacctttg aatgaagtaa acagtttccc acaaaaaatc agctatacac
     3661 aattcccaaa ctcattttat gagcctcatc aagattttac cagtccagat atattcaaga
     3721 agtcaagatc tccatcttgg tataaataca cttccacagt cagcacgggg atcacagact
     3781 tagaaagttc aactggactt tggcctacaa tttcccagtt tactcttagt gaagagacaa
     3841 acgcagatgt ttattattat cgcatcatca tacctgtcct tttgatgtta gtatttcttg
     3901 ctttattttt tctctaggac ccagctttct tgtacaaagt tgtgatatcc agcacagtgg
     3961 cggccgctcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4021 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4081 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4141 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4201 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4261 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4321 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     4381 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4441 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4501 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4561 cctggaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     4621 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     4681 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     4741 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     4801 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     4861 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     4921 gaggctggga aggggtgggt ccgggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     4981 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     5041 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
     5101 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5161 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     5221 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     5281 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     5341 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5401 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     5461 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5521 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5581 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5641 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     5701 cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg tacctttaag
     5761 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5821 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     5881 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5941 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6001 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     6061 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     6121 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6181 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6241 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     6301 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6361 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6421 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     6481 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     6541 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     6601 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     6661 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6721 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6781 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6841 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6901 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     6961 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7021 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     7081 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7141 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7201 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7261 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7321 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7381 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7441 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7501 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7561 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7621 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7681 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7741 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7801 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7861 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7921 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7981 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8041 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8101 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8161 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8221 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8281 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8341 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8401 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8461 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8521 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8581 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8641 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8701 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8761 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8821 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8881 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8941 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9001 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9061 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     9121 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     9181 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     9241 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     9301 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     9361 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     9421 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     9481 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     9541 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     9601 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     9661 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     9721 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     9781 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     9841 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     9901 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     9961 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
    10021 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
    10081 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
    10141 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
    10201 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
    10261 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
    10321 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
    10381 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
    10441 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
    10501 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
    10561 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
    10621 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
    10681 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    10741 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    10801 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    10861 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    10921 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgtt
//
