LOCUS       sequence-314667-       11027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CAMKK1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11027)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CAMKK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..11027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    80..196
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    230..250
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        250..1461
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1298..1317
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1465..1485)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1486..1495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1498..2268
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2275..2316
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2317..2341
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3911..3935)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3965..4553
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4018..4038)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4436..4447)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4567..5066
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4602..4625)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4999..5018
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5087..5686
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5087..5106)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5583..5603
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5824..6057
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6129..6250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6166..6185)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6220..6239
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6290..6425
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6445..6464)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6446..6464)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6474..6491)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6474..6490)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6483..6505)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6632..7087
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6719..6738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6929..6950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7113..7217
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7218..7286,7287..8078)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8249..8837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8738..8757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8991..9008
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9125..9146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9161..9166,9167..9184,9185..9191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9199..9215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9204..9226
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9223..9239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9223..9239
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9258..9278
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9260..9278
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9306..9532
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9533..9713
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9760..9885
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10378..10611
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10796..10840
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
       61 ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg
      121 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
      181 caaaattcaa aattttatcg tccagtgtgg tggaattctg cagatatcac aactttgtat
      241 agaaaagttg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg
      301 cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc
      361 tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg
      421 acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa
      481 caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg
      541 aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg
      601 gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc
      661 tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg
      721 agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg
      781 gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc
      841 gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct
      901 tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct
      961 ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg
     1021 ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct
     1081 cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg
     1141 gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct
     1201 ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt
     1261 ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg
     1321 cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca
     1381 tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg
     1441 tttttggctt ttttgttaga cggccaactt ttgtatacaa agtgggccac catgggaatc
     1501 ccgctgctga acgctaaaca gattctggga cagctggacg gcgggagcgt ggcagtcctg
     1561 cctgtggtcg actccaccaa tcagtacctg ctggatcgaa tcggcgagct gaagagtggg
     1621 gatgcttgca ttgcagaata tcagcaggca gggagaggaa gcagagggag gaaatggttc
     1681 tctccttttg gagctaacct gtacctgagt atgttttggc gcctgaagcg gggaccagca
     1741 gcaatcggcc tgggcccggt catcggaatt gtcatggcag aagcgctgcg aaagctggga
     1801 gcagacaagg tgcgagtcaa atggcccaat gacctgtatc tgcaggatag aaagctggca
     1861 ggcatcctgg tggagctggc cggaataaca ggcgatgctg cacagatcgt cattggcgcc
     1921 gggattaacg tggctatgag gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca
     1981 ctgcaggaag cagggattaa cctggacagg aatactctgg ccgctatgct gatccgagag
     2041 ctgcgggcag ccctggaact gttcgagcag gaaggcctgg ctccatatct gtcacggtgg
     2101 gagaagctgg ataacttcat caatagaccc gtgaagctga tcattgggga caaagagatt
     2161 ttcgggatta gccgggggat tgataaacag ggagccctgc tgctggaaca ggacggagtt
     2221 atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag
     2281 cccatcccca accccctgct gggcctggac agcaccacaa gtttgtacaa aaaagcaggc
     2341 tccaccatgg aggggggtcc agctgtctgc tgccaggatc ctcgggcaga gctggtagaa
     2401 cgggtggcag ccatcgatgt gactcacttg gaggaggcag atggtggccc agagcctact
     2461 agaaacggtg tggacccccc accacgggcc agagctgcct ctgtgatccc tggcagtact
     2521 tcaagactgc tcccagcccg gcctagcctc tcagccagga agctttccct acaggagcgg
     2581 ccagcaggaa gctatctgga ggcgcaggct gggccttatg ccacggggcc tgccagccac
     2641 atctcccccc gggcctggcg gaggcccacc atcgagtccc accacgtggc catctcagat
     2701 gcagaggact gcgtgcagct gaaccagtac aagctgcaga gtgagattgg caagggtgcc
     2761 tacggtgtgg tgaggctggc ctacaacgaa agtgaagaca gacactatgc aatgaaagtc
     2821 ctttccaaaa agaagttact gaagcagtat ggctttccac gtcgccctcc cccgagaggg
     2881 tcccaggctg cccagggagg accagccaag cagctgctgc ccctggagcg ggtgtaccag
     2941 gagattgcca tcctgaagaa gctggaccac gtgaatgtgg tcaaactgat cgaggtcctg
     3001 gatgacccag ctgaggacaa cctctatttg gccctgcaga accaggccca gaatatccag
     3061 ttagattcaa caaatatcgc caagccccac tccctgcttc cctctgagca gcaagacagt
     3121 ggatccacgt gggctgcgcg ctcagtgttt gacctcctga gaaaggggcc cgtcatggaa
     3181 gtgccctgtg acaagccctt ctcggaggag caagctcgcc tctacctgcg ggacgtcatc
     3241 ctgggcctcg agtacttgca ctgccagaag atcgtccaca gggacatcaa gccatccaac
     3301 ctgctcctgg gggatgatgg gcacgtgaag atcgccgact ttggcgtcag caaccagttt
     3361 gaggggaacg acgctcagct gtccagcacg gcgggaaccc cagcattcat ggcccccgag
     3421 gccatttctg attccggcca gagcttcagt gggaaggcct tggatgtatg ggccactggc
     3481 gtcacgttgt actgctttgt ctatgggaag tgcccgttca tcgacgattt catcctggcc
     3541 ctccacagga agatcaagaa tgagcccgtg gtgtttcctg aggggccaga aatcagcgag
     3601 gagctcaagg acctgatcct gaagatgtta gacaagaatc ccgagacgag aattggggtg
     3661 ccagacatca agttgcaccc ttgggtgacc aagaacgggg aggagcccct tccttcggag
     3721 gaggagcact gcagcgtggt ggaggtgaca gaggaggagg ttaagaactc agtcaggctc
     3781 atccccagct ggaccacggt gatcctggtg aagtccatgc tgaggaagcg ttcctttggg
     3841 aacccgtttg agccccaagc acggagggaa gagcgatcca tgtctgctcc aggaaaccta
     3901 ctggtgtagg acccagcttt cttgtacaaa gtggtgatat ccagcacagt ggcggccgct
     3961 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4021 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4081 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4141 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4201 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4261 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4321 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     4381 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4441 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4501 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     4561 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     4621 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     4681 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     4741 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     4801 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     4861 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     4921 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     4981 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     5041 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa
     5101 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     5161 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     5221 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5281 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5341 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5401 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5461 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5521 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5581 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5641 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     5701 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt
     5761 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5821 gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5881 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5941 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6001 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6061 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6121 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6241 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     6301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6421 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     6481 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6541 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6601 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     6661 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6721 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6781 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6841 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6901 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6961 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7021 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7081 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7141 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7201 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7261 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7321 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7381 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7441 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7501 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7561 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7621 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7681 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7741 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7801 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7861 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7921 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7981 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8041 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8101 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8161 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8221 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8281 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8341 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8401 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8461 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8521 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8581 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8641 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8701 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8761 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8821 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8881 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8941 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9001 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9061 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     9241 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     9301 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     9361 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     9421 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     9481 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     9541 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9601 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9661 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     9721 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     9781 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     9841 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     9901 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     9961 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    10021 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    10081 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    10141 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    10201 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
    10261 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
    10321 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
    10381 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    10441 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    10501 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    10561 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    10621 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    10681 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    10741 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    10801 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    10861 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    10921 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
    10981 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaag
//
