LOCUS       sequence-31467-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SIRT5
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             60..77
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
       61 tggttccgcg tggtagtgct cggccaagtt caagtatggc agattttcga aagttttttg
      121 caaaagcaaa gcacatagtc atcatctcag gagctggtgt tagtgcagaa agtggtgttc
      181 cgaccttcag aggagctgga ggttattgga gaaaatggca agcccaggac ctggcgactc
      241 ccctggcctt tgcccacaac ccgtcccggg tgtgggagtt ctaccactac cggcgggagg
      301 tcatggggag caaggagccc aacgccgggc accgcgccat agccgagtgt gagacccggc
      361 tgggcaagca gggccggcga gtcgtggtca tcacccagaa catcgatgag ctgcaccgca
      421 aggctggcac caagaacctt ctggagatcc atggtagctt atttaaaact cgatgtacct
      481 cttgtggagt tgtggctgag aattacaaga gtccaatttg tccagcttta tcaggaaaag
      541 gtgctccaga acctggaact caagatgcca gcatcccagt tgagaaactt ccccggtgtg
      601 aagaggcagg ctgcgggggc ttgctgcgac ctcatgtcgt gtggtttgga gaaaacctgg
      661 atcctgccat tctggaggag gttgacagag agctcgccca ctgtgattta tgtctagtgg
      721 tgggcacttc ctctgtggtg tacccagcag ccatgtttgc cccccaggtg gctgccaggg
      781 gcgtgccagt ggctgaattt aacacggaga ccaccccagc tacgaacaga ttcaggtttc
      841 a
//
