LOCUS       sequence-31469-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: WDR5 (2GNQ)
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccctctgaat attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggttccgc gtggtagtat ggcgacggag gagaagaagc
      121 ccgagaccga ggccgccaga gcacagccaa ccccttcgtc atccgccact cagagcaagc
      181 ctacacctgt gaagccaaac tatgctctaa agttcaccct tgctggccac accaaagcag
      241 tgtcctccgt gaaattcagc ccgaatggag agtggctggc aagttcatct gctgataaac
      301 ttattaaaat ttggggcgcg tatgatggga aatttgagaa aaccatatct ggtcacaagc
      361 tgggaatatc cgatgtagcc tggtcgtcag attctaacct tcttgtttct gcctcagatg
      421 acaaaacctt gaagatatgg gacgtgagct cgggcaagtg tctgaaaacc ctgaagggac
      481 acagtaatta tgtcttttgc tgcaacttca atccccagtc caaccttatt gtctcaggat
      541 cctttgacga aagcgtgagg atatgggatg tgaaaacagg gaagtgcctc aagactttgc
      601 cagctcactc ggatccagtc tcggccgttc attttaatcg tgatggatcc ttgatagttt
      661 caagtagcta tgatggtctc tgtcgcatct gggacaccgc ctcgggccag tgcctgaaga
      721 cgctcatcga tgacgacaac ccccccgtgt cttttgtgaa gttctccccg aacggcaaat
      781 acatcctggc cgccacgctg gacaacactc tgaagctctg ggactacagc aaggggaagt
      841 gcctgaagac gtacactggc cacaagaatg agaaatactg catatttgcc aatttctctg
      901 ttact
//
