LOCUS       sequence-314697-        1269 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of ultrafast protein calcium sensor under 
            the Syn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Zhang Y, Rozsa M, Bushey D, Zheng J, Reep D, Broussard GJ, Tsang A, 
            Tsegaye G, Patel R, Narayan S, Li JX, Zhang R, Ahrens MB, Turner GC,
            Wang SS-H, Svoboda K, Korff W, Schreiter ER, Hasseman JP, Kolb I, 
            Looger LL
  TITLE     jGCaMP8 Fast Genetically Encoded Calcium Indicators
  JOURNAL   Janelia Research Campus, Online Resource (2020) 
            doi:10.25378/janelia.13148243.
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Janelia 
            Research Campus, Online Resource (2020) 
            doi:10.25378/janelia.13148243."
COMMENT     Sequence Label: pGP-AAV-syn-jGCaMP8s-WPRE
FEATURES             Location/Qualifiers
     source          1..1269
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     303..324
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(430..451)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(691..710)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgcatcatc accatcatca cacgcgtcgc aagaagacct tcaaggaggt ggccaccgcc
       61 gtgaagatca tcgccatgct gatgggcctg aagatcaacg tctatatcaa ggccgacaag
      121 cagaagaacg gcatcaaggc gaacttccac atccgccaca acatcgagga cggcggcgtg
      181 cagctcgcct accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      241 gacaaccact acctgagcgt ggagtccaaa ctttcgaaag accccaacga gaagcgcgat
      301 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      361 tacaagggcg gtaccggagg gagcatggtg agcaagggcg aggagctgtt caccggggtg
      421 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      481 gagggtgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      541 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      601 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      661 tacatccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      721 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      781 gaggacggca acatcctggg gcacaagctg gagtacaacc tgccggacca actgactgaa
      841 gagcagatcg cagaatacaa agaggctttc tccctatttg acaaggacgg ggatgggaca
      901 ataacaacca aggagctggg gacggtgatg cggtctctcg gacacaaccc cacagaagca
      961 gagctgcagg acatgatcaa tgaagtagat gccgacggtg acggcacaat cgacttccct
     1021 gagttcctga caatgatggc aagaaaaatg aaatacaggg acacggaaga agaaattaga
     1081 gaagcgttcg gtgtgtttga taaggatggc aatggctaca tcagtgcagc agagcttcgc
     1141 cacgtgatga caaaccttgg agagaagtta acagatgaag aggttgatga aatgatcagg
     1201 gaagcagaca tcgatgggga tggtcaggta aactacgaag agtttgtaca aatgatgaca
     1261 gcgaagtaa
//
