LOCUS       sequence-314713-        1269 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of ultrafast protein calcium sensor under 
            the CAG promoter, Cre-dependent expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Zhang Y, Rozsa M, Bushey D, Zheng J, Reep D, Broussard GJ, Tsang A, 
            Tsegaye G, Patel R, Narayan S, Li JX, Zhang R, Ahrens MB, Turner GC,
            Wang SS-H, Svoboda K, Korff W, Schreiter ER, Hasseman JP, Kolb I, 
            Looger LL
  TITLE     jGCaMP8 Fast Genetically Encoded Calcium Indicators
  JOURNAL   Janelia Research Campus, Online Resource (2020) 
            doi:10.25378/janelia.13148243.
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Janelia 
            Research Campus, Online Resource (2020) 
            doi:10.25378/janelia.13148243."
COMMENT     Sequence Label: pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE
FEATURES             Location/Qualifiers
     source          1..1269
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     560..579
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     819..840
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(946..967)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1249..1266)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcacttcgct gtcatcattt gtacaaactc ttcgtagttt acctgaccat ccccatcgat
       61 gtctgcttcc ctgatcattt catcaacctc ttcatctgtt aacttctctc caaggtttgt
      121 catcacgtgg cgaagctctg ctgcactgat gtagccattg ccatccttat caaacacacc
      181 gaacgcttct ctaatttctt cttccgtgtc cctgtatttc atttttcttg ccatcattgt
      241 caggaactca gggaagtcga ttgtgccgtc accgtcggca tctacttcat tgatcatgtc
      301 ctgcagctct gcttctgtgg ggttcagtcc gagagaccgc atcaccgtcc ccagctcctt
      361 ggttgttatt gtcccatccc cgtccttgtc aaatagggag aaagcctctt taaattctgc
      421 gatctgctct tcagtcagtt ggtccggcag gttgtactcc agcttgtgcc ccaggatgtt
      481 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
      541 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
      601 ctggatgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
      661 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac
      721 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
      781 ctcaccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      841 gatgggcacc accccggtga acagctcctc gcccttgctc accatgctcc ctccggtacc
      901 gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      961 gaccatgtga tcgcgcttct cgttggggtc tttcgaaagt ttggactcca cgctcaggta
     1021 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggta
     1081 ggcgagctgc acgccgccgt cctcgatgtt gtggcggatg tggaagttcg ccttgatgcc
     1141 gttcttctgc ttgtcggcct tgatatagac gttgatcttc aggcccatca ggcgggcgat
     1201 gatcttcacg gcggtggcca cctccttgaa ggtcttcttg cgacgcgtgt gatgatggtg
     1261 atgatgcat
//
