LOCUS       sequence-314726-       11354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of DYRK1B (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11354)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-DYRK1B_IDG-K
FEATURES             Location/Qualifiers
     source          1..11354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    108..224
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    258..278
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        278..1489
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1326..1345
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1493..1513)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1514..1523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1526..2296
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2303..2344
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2345..2369
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4266..4290)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4320..4908
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4373..4393)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4791..4802)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4922..5421
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4957..4980)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5354..5373
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5442..6041
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5442..5461)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5938..5958
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6179..6412
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6484..6605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6521..6540)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6575..6594
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6645..6780
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6800..6819)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6801..6819)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6829..6846)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6829..6845)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6838..6860)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6987..7442
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7074..7093)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7284..7305
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7468..7572
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7573..7641,7642..8433)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8604..9192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9093..9112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9346..9363
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9480..9501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9516..9521,9522..9539,9540..9546)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9554..9570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9559..9581
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9578..9594
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9578..9594
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9613..9633
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9615..9633
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9661..9887
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9888..10068
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10115..10240
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10733..10966
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11151..11195
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
       61 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
      121 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      181 caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc cagtgtggtg
      241 gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg ggttttggcg
      301 cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc
      361 gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg
      421 gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg
      481 gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat
      541 tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt
      601 gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc
      661 tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg
      721 ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc
      781 gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg
      841 agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg
      901 catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat
      961 tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg
     1021 gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg
     1081 cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc
     1141 ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag
     1201 gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg
     1261 aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa
     1321 gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt
     1381 tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac
     1441 tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg gccaactttt
     1501 gtatacaaag tgggccacca tgggaatccc gctgctgaac gctaaacaga ttctgggaca
     1561 gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc agtacctgct
     1621 ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc agcaggcagg
     1681 gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt acctgagtat
     1741 gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca tcggaattgt
     1801 catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat ggcccaatga
     1861 cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg gaataacagg
     1921 cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc gcgtggagga
     1981 aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc tggacaggaa
     2041 tactctggcc gctatgctga tccgagagct gcgggcagcc ctggaactgt tcgagcagga
     2101 aggcctggct ccatatctgt cacggtggga gaagctggat aacttcatca atagacccgt
     2161 gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg ataaacaggg
     2221 agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag aaatcagtct
     2281 gcggtctgcc gaaaaggcta gcggcaagcc catccccaac cccctgctgg gcctggacag
     2341 caccacaagt ttgtacaaaa aagcaggctc caccatggcc gtcccaccgg gccatggtcc
     2401 cttctctggc ttcccagggc cccaggagca cacgcaggta ttgcctgatg tgcggctact
     2461 gcctcggagg ctgcccctgg ccttccggga tgcaacctca gccccgctgc gtaagctctc
     2521 tgtggacctc atcaagacct acaagcacat caatgaggta tactatgcga agaagaagcg
     2581 gcgggcccag caggcgccac cccaggattc gagcaacaag aaggagaaga aggtcctgaa
     2641 ccatggttat gatgacgaca accatgacta catcgtgcgc agtggcgagc gctggctgga
     2701 gcgctacgaa attgactcgc tcattggcaa aggctccttt ggccaggtgg tgaaagccta
     2761 tgatcatcag acccaggagc ttgtggccat caagatcatc aagaacaaaa aggctttcct
     2821 gaaccaggcc cagattgagc tgcggctgct ggagctgatg aaccagcatg acacggagat
     2881 gaagtactat atagtacacc tgaagcggca cttcatgttc cggaaccacc tgtgcctggt
     2941 atttgagctg ctgtcctaca acctgtacga cctcctgcgc aacacccact tccgcggcgt
     3001 ctcgctgaac ctgacccgga agctggcgca gcagctctgc acggcactgc tctttctggc
     3061 cacgcctgag ctcagcatca ttcactgcga cctcaagccc gaaaacatct tgctgtgcaa
     3121 ccccaagcgc agcgccatca agattgtgga cttcggcagc tcctgccagc ttggccagag
     3181 gatctaccag tatatccaga gccgcttcta ccgctcacct gaggtgctcc tgggcacacc
     3241 ctacgacctg gccattgaca tgtggtccct gggctgcatc cttgtggaga tgcacaccgg
     3301 agagcccctc ttcagtggct ccaatgaggt cgaccagatg aaccgcattg tggaggtgct
     3361 gggcatccca ccggccgcca tgctggacca ggcgcccaag gctcgcaagt actttgaacg
     3421 gctgcctggg ggtggctgga ccctacgaag gacgaaagaa ctcaggaagg attaccaggg
     3481 ccccgggaca cggcggctgc aggaggtgct gggcgtgcag acgggcgggc ccgggggccg
     3541 gcgggcgggg gagccgggcc acagccccgc cgactacctc cgcttccagg acctggtgct
     3601 gcgcatgctg gagtatgagc ccgccgcccg catcagcccc ctgggggctc tgcagcacgg
     3661 cttcttccgc cgcacggccg acgaggccac caacacgggc ccggcaggca gcagtgcctc
     3721 cacctcgccc gcgcccctcg acacctgccc ctcttccagc accgccagct ccatctccag
     3781 ttctggaggc tccagtggct cctccagtga caaccggacc taccgctaca gcaaccgata
     3841 ttgtgggggc cctgggcccc ctatcacaga ctgtgagatg aacagccccc aggtcccacc
     3901 ctcccagccg ctgcggccct gggcaggggg tgatgtgccc cacaagacac atcaagcccc
     3961 tgcctctgcc tcgtcactgc ctgggaccgg ggcccagtta cccccccagc cccgatacct
     4021 tggtcgtccc ccatcaccaa cctcaccacc acccccggag ctgatggatg tgagcctggt
     4081 gggcggccct gctgactgct ccccacctca cccagcgcct gccccccagc acccggctgc
     4141 ctcagccctc cggactcgga tgactggagg tcgtccaccc ctcccgcctc ctgatgaccc
     4201 tgccactctg gggcctcacc tgggcctccg tggtgtaccc cagagcacag cagccagctc
     4261 gtaggaccca gctttcttgt acaaagtggt gatatccagc acagtggcgg ccgctcgaca
     4321 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4381 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4441 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4501 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4561 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4621 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4681 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     4741 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4801 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4861 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctac
     4921 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     4981 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     5041 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     5101 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     5161 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     5221 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     5281 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     5341 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     5401 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca
     5461 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     5521 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     5581 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5641 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5701 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     5761 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     5821 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     5881 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     5941 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6001 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     6061 agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt acctttaaga
     6121 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6181 gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6421 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6481 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6541 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6601 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     6661 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6721 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6781 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     6841 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     6901 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     6961 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     7021 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7081 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7141 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7201 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7261 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7321 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7381 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     7441 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7501 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7561 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7621 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7681 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7741 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7801 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7861 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7921 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7981 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8041 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8101 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8161 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8221 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8281 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8341 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8401 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8461 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8521 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8581 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8641 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8701 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8761 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8821 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8881 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8941 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9001 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9061 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9121 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9181 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9241 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9301 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9361 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     9421 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9481 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9541 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     9601 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     9661 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     9721 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     9781 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     9841 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     9901 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9961 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10021 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
    10081 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
    10141 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
    10201 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
    10261 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
    10321 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
    10381 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
    10441 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
    10501 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
    10561 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
    10621 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
    10681 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
    10741 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
    10801 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
    10861 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
    10921 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
    10981 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
    11041 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
    11101 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
    11161 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
    11221 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    11281 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    11341 agacccacct ccca
//
