LOCUS       sequence-314732-         808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of proteins with a C-terminal thermostable GFP (TGP) tag 
            in insect cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Cai H, Yao H, Li T, Hutter CAJ, Li Y, Tang Y, Seeger MA, Li D
  TITLE     An improved fluorescent tag and its nanobodies for membrane protein 
            expression, stability assay, and purification.
  JOURNAL   Commun Biol. 2020 Dec 10;3(1):753. doi: 10.1038/s42003-020-01478-z.
  PUBMED    33303987
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-12-10"; volume: "3(1):753. doi"; pages: " 
            10.1038/s42003-020-01478-z"
COMMENT     Sequence Label: pFTSG
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             22..45
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             739..762
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 cgcgaattcc tcgagggtac cctggaagtg ctgtttcagg gcccgggcgg tagtggaggt
       61 tccgcgagtg tcattaagcc ggagatgaaa attaagttgc gtatggaggg ggccgtgaat
      121 gggcacaaat tcgttattga aggggagggg attggaaagc cgtatgaggg tacgcaaacc
      181 ttggacttaa cagtagaaga aggggcacca ttgccttttt cttatgatat tttgactccg
      241 gcgttccaat acgggaaccg tgctttcaca aagtatcccg aagatatccc tgactatttc
      301 aagcaagcat ttcccgaggg ttacagttgg gagagaagta tgacctatga ggatcaaggc
      361 atatgtattg ctacctctga cattactatg gagggagact gcttcttcta tgagattcgt
      421 ttcgatggaa caaactttcc tcctaacggg ccagtgatgc agaaaaagac gttgaagtgg
      481 gaaccgtcca cagaaaaaat gtatgtcgag gacggcgttc taaagggaga cgtggaaatg
      541 gccttgctat tggaaggggg tggacactat cgttgtgatt tcaaaacgac ctacaaggcc
      601 aaaaaagatg tgagactacc ggacgcccac gaggttgacc atcgtattga aattttaagt
      661 cacgacaagg actataataa agtaagactg tacgaacacg cggaagcacg ttacagcgga
      721 ggtggaagtg gtggtggcca ccatcaccat caccatcacc attaagtcga gaagtactag
      781 aggatcataa tcagccatac cacatttg
//
