LOCUS       sequence-31474-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SAT
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             629..646
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(699..717)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      713..760
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atattttgtt tactttaaga aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggttccg cgtggtagtg ctaaattcgt gatccgccca gccactgccg
      121 ccgactgcag tgacatactg cggctgatca aggagctggc taaatatgaa tacatggaag
      181 aacaagtaat cttaactgaa aaagatctgc tagaagatgg ttttggagag cacccctttt
      241 accactgcct ggttgcagaa gtgccgaaag agcactggac tccggaagga cacagcattg
      301 ttggttttgc catgtactat tttacctatg acccgtggat tggcaagtta ttgtatcttg
      361 aggacttctt cgtgatgagt gattatagag gctttggcat aggatcagaa attctgaaga
      421 atctaagcca ggttgcaatg aggtgtcgct gcagcagcat gcacttcttg gtagcagaat
      481 ggaatgaacc atccatcaac ttctataaaa gaagaggtgc ttctgatctg tccagtgaag
      541 agggttggag actgttcaag atcgacaagg agtacttgct aaaaatggca acagaggagt
      601 gatgacgaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc
      661 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      721 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      781 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      841 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgc
//
